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Extraction and preparation method of high-molecular-weight tremella polysaccharide

A tremella polysaccharide, high molecular weight technology, applied in the field of extraction and preparation of high molecular weight tremella polysaccharide, can solve the problems of tremella polysaccharide decomposition structure, damage, etc.

Active Publication Date: 2021-04-30
山东百阜福瑞达制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems and overcome the deficiencies in the prior art, the present invention provides a method for extracting and preparing high molecular weight tremella polysaccharides, which can effectively solve the problem that tremella polysaccharides are prone to decomposition and structural damage during acid-base extraction

Method used

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Examples

Experimental program
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specific Embodiment approach

[0027] Z1. Add tremella powder to the reaction tank that has been filled with water and heated to 38-40°C, and add chitinase to break the wall;

[0028] Z2. Adding tannic acid or trichloroacetic acid to the reaction tank for protein removal treatment, the amount of tannic acid or trichloroacetic acid added is 0.2-0.3‰ of the treatment liquid quality;

[0029] Z3. Raise the temperature of the reaction tank to 55-60°C to extract the tremella polysaccharide from the broken cells; this can shorten the extraction time from 4 hours to 2 hours;

[0030] Z4. Filtrate with a 0.22 μm filter cardboard to remove Tremella fungus protoplasts containing glycoproteins to obtain Tremella polysaccharide extract;

[0031] Z5. Add the tremella polysaccharide filtrate to the concentration equipment for concentration, and concentrate to a concentrated viscosity of 900-1100mm 2 / s;

[0032] Z6, adding the concentrated filtrate to absolute ethanol to precipitate the feed liquid, and extract pure wh...

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PUM

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Abstract

The invention discloses an extraction and preparation method of high-molecular-weight tremella polysaccharide, and belongs to the technical field of bioactive substance extraction. The method is characterized by comprising a wall breaking process and a deproteinization process, and further comprises an extraction process, a separation process, a concentration process and a purification process after the deproteinization process. According to the invention, chitinase is used for wall breaking treatment, so that complete cell membranes, namely protoplasts, with biological activity can be effectively reserved while cell walls are removed; glycoproteins containing tremella polysaccharide and wound on the tremella polysaccharide are isolated in the protoplasts, tannic acid or trichloroacetic acid can smoothly pass through the cell membranes to act on the glycoprotein wound on the tremella polysaccharide, and the glycoprotein is separated from the tremella polysaccharide after the glycoprotein is denatured; and the separated tremella polysaccharide can smoothly pass through a cell membrane to the outside of the cell membrane due to the characteristic of water solubility, so that the purpose of separating the glycoprotein from the tremella polysaccharide after the glycoprotein is denatured is achieved, and high yield and low protein impurities of the tremella polysaccharide are guaranteed.

Description

Technical field: [0001] The invention belongs to the technical field of bioactive substance extraction, and more specifically relates to an extraction and preparation method of high-molecular-weight white fungus polysaccharide. Background technique: [0002] Tremella fucifor (nis Berk), also known as white fungus, white fungus, chrysanthemum ear, mainly contains protein, carbohydrates, vitamins and various amino acids, etc., and can be used for medicine and nourishing food. Tremella is mainly produced in Sichuan, Guizhou, Hubei, Shaanxi, Fujian, Zhejiang and other provinces. Tremella polysaccharide, one of the main active components of fruiting bodies, has gradually become a research hotspot in recent years. Modern studies have confirmed that tremella polysaccharides are mainly heteropolysaccharides with α-(1-3)-D mannose as the main chain, and have various biological activities such as immune regulation, anti-tumor, anti-oxidative aging, lowering blood sugar and blood lipid...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 刘磊张俊停康传利李庆屈爱存李焕成杜帅刘蔷张美霞廉少杰李庆攀汤丽伟
Owner 山东百阜福瑞达制药有限公司
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