A kind of Trichoderma cereus and application thereof
A technology of Trichoderma and strains, applied in the field of microorganisms, can solve problems such as environmental pollution and waste of resources, and achieve the effects of solving environmental pollution, increasing production, and improving quality
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Embodiment 1
[0037] The present embodiment provides a kind of Trichoderma cerinaceum, which is collected and isolated from the rhizosphere soil of Magnolia cereus in Lijiang City, Yunnan Province. The Latin scientific name of the above-mentioned Trichoderma erinaceum is Trichoderma erinaceum, the strain number is TR1186A, and the preservation number is CGMCCNo. 21427.
[0038] The Trichoderma erinaceum strain of the present invention is preserved in the General Microorganism Center (CGMCC) of the China Microorganism Culture Collection Management Committee, and the preservation address is: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; preservation date 2021.01 13th of May.
[0039] Classification and identification of Trichoderma cereus:
[0040] The above-mentioned Trichoderma cereus grows on the MA medium at 20°C, the colony diameter is 56-60mm after 72 hours, the diameter is 26-32mm after 36°C, 65 hours, does not grow at 40°C, and has no aerial mycelium, such as Some are...
Embodiment 2
[0053] The present embodiment provides a Trichoderma cereus fermentation supernatant, and the preparation method of the above-mentioned Trichoderma cereus fermentation supernatant includes the following steps:
[0054] Step 1, the Trichoderma cereus seed isolated from Example 1 is inoculated in a PDA medium, and cultivated at a constant temperature;
[0055] In step 2, the strains cultivated in step 1 are transferred to the culture solution for culturing in shake flasks for 24 hours to obtain first-class seed solution;
[0056] Step 3, after sterilizing the culture liquid in the intermediate fermentation tank at 121 ° C and 15 pounds of pressure for 20 min, maintaining positive pressure, cooling to 30 ° C, then pouring the first-class seed liquid obtained in step 2 into the intermediate fermentation tank, adding Sterile air is introduced under pressure, and fermentation is carried out for 6 days at 28-30°C, 0.4-0.5kg pressure, 1500-1800L / H air flow and 120-150rpm rotating spee...
Embodiment 3
[0064] The present embodiment provides a water-soluble fertilizer with a large amount of elements, which corresponds to the Trichoderma cereus fermentation supernatant obtained by adopting the culture solutions of groups 1-3 in the above-mentioned embodiment 2, respectively including the following (Table 1) components by weight percentage :
[0065] Table 1
[0066] Example 3-1 Example 3-2 Example 3-3 Trichoderma cereus fermentation supernatant 42.48％ 40.82％ 44.89％ Potassium nitrate 10.6％ 9.66％ 10.21％ Urea 12.8％ 13.5％ 9.55％ Dipotassium phosphate 10.5％ 12.4％ 11.85％ Ammonium polyphosphate 9.5％ 8.5％ 10.0％ Molasses Powder 9.12％ 12.12％ 8.5％ Alginic acid 5％ 3％ 5％
[0067] The above-mentioned large-scale element water-soluble fertilizer is prepared by the following method: according to the proportioning, the Trichoderma cereus fermentation supernatant is put into the batching tank, then the stirring device is...
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