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Acanthus ilicifolius endophytic fungus Diamorpha gigantei and application of metabolite of acanthus ilicifolius endophytic fungus

A technology of endophytic fungus and rat pombe, applied in the field of microorganisms, can solve the problems of no discovery and achieve obvious anti-tumor activity

Pending Publication Date: 2021-05-07
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far nothing has been found about endophytic fungi Diaporthe goulteri report

Method used

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  • Acanthus ilicifolius endophytic fungus Diamorpha gigantei and application of metabolite of acanthus ilicifolius endophytic fungus
  • Acanthus ilicifolius endophytic fungus Diamorpha gigantei and application of metabolite of acanthus ilicifolius endophytic fungus
  • Acanthus ilicifolius endophytic fungus Diamorpha gigantei and application of metabolite of acanthus ilicifolius endophytic fungus

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Embodiment 1

[0029] The applicant has collected a batch of fresh rat bougainvillea roots, stems and leaves from Shankou Mangrove National Reserve in Guangxi, and it is separated and purified respectively through the following steps:

[0030] (1) For the freshly collected roots, stems and leaves of the rat bougainvillea, wash off the surface soil and dust, and dry them with sterile filter paper.

[0031] (2) Put the roots, stems and leaves of the above mouse bougainvillea in the ultra-clean workbench according to the following procedures: soak in 75% ethanol for 1 min → wash with 0.1% mercury liter for 1 min → rinse with sterile water for 3 times → absorb the water with filter paper → materials Cut into small pieces of about 0.5×0.5 cm, inoculate them on PDA medium, and culture them statically in a constant temperature incubator at 25°C for 4-7 days to observe the growth of endophytic fungi.

[0032] (3) Pick the tip of the mycelium at the edge of the tissue and transfer it to a new PDA med...

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Abstract

The invention discloses an acanthus ilicifolius endophytic fungus Diamorpha gigantei and application of a metabolite of the acanthus ilicifolius endophytic fungus Diamorpha gigantei, the acanthus ilicifolius endophytic fungus Diamorpha gigantei is a fungus separated from acanthus ilicifolius roots, the species name of the acanthus ilicifolius endophytic fungus Diamorpha gigantei is Diamorpha gigantei, and the nucleotide sequence of an ITS region of the fungus is shown as SEQ ID No.1. The invention further discloses a preparation method of the acanthus ilicifolius endophytic fungus Diamorpha gigantei. According to the Acanthus ilicifolius endophytic fungus Diamorpha gigantei and the application of the metabolite of the acanthus ilicifolius endophytic fungus, a new endophytic fungus variety is separated from the acanthus ilicifolius roots, the variety of known mangrove endophytic fungi is enriched, the ethyl acetate extract of the secondary metabolite obtained by fermentation of the fungus has obvious antitumor activity through cell activity test verification, and a new material basis is provided for research of novel targeting drugs for tumors.

Description

technical field [0001] The invention belongs to the field of microorganisms and the field of tumor treatment, in particular to an endophytic fungus strain of rat bougainvillea Diaporthe goulteri and the application of its metabolites. Background technique [0002] Endophytic fungi ( Endophytic fungi ) are a group of fungi that colonize healthy plant tissues but do not cause plant disease symptoms. Plant endophytic fungi are an important part of biodiversity in nature. As one of the four major marine ecosystems, mangroves grow in a coastline environment characterized by strong reduction, strong acidity, high salinity, and rich nutrients, creating microbial resources with marine microbial characteristics. Mangroves are rich in fungal resources. More than 400 species of mangrove fungi have been isolated and identified, making them the second largest group of marine fungi. Mangrove endophytic fungi parasitize in the mangrove plant tissue and have an antagonistic effect on...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02A61K36/062A61P35/00C12R1/645
CPCC12N1/14C12N1/02A61K36/062A61P35/00
Inventor 朱丹刘妍颖严石焕康亮郭宏伟苏志恒
Owner GUANGXI MEDICAL UNIVERSITY
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