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Kit for detecting urothelial carcinoma by identifying UMOD-modified Neu5Gc in urine based on LIP

A technology of urothelial carcinoma and kit, applied in the field of biomedical detection, to achieve the effect of good clinical specimen detection ability, high sensitivity and specificity, and simple operation

Active Publication Date: 2021-05-07
LIAONING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, so far, there is no report on the detection of urothelial carcinoma based on Neu5Gc, which uses sialic acid-modified UMOD as a marker to identify UMOD-modified urine by LIP

Method used

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  • Kit for detecting urothelial carcinoma by identifying UMOD-modified Neu5Gc in urine based on LIP
  • Kit for detecting urothelial carcinoma by identifying UMOD-modified Neu5Gc in urine based on LIP

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experiment example 1

[0045] Adopt the present invention to implement 639 cases of normal people, 25 cases of benign diseases (8 cases of bladder inflammation, 7 cases of bladder cyst, 6 cases of bladder polyps, 4 cases of nephritis), 99 cases of urothelial cancer patients (52 cases of bladder cancer, 20 cases of renal cancer and 27 cases of prostate cancer) urine test samples were tested and the results were as follows figure 1 shown. figure 1 It showed that the Neu5Gc content of LIP-specific recognition modified UMOD glycoprotein in normal human urine samples was 67.63±9.31 ng / mg; the Neu5Gc content of LIP-specific recognition modified UMOD glycoprotein in 25 cases of benign disease urine samples was not significantly higher than that of normal people. Sexual differences, ranging from 70.71±8.20 ng / mg. The results showed that: in the screening of urinary tract cancer, when there are benign problems such as inflammation in the urinary tract, there is no difference between the content of Neu5Gc th...

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Abstract

The invention discloses a kit for detecting urothelial carcinoma by identifying UMOD-modified Neu5Gc in urine based on LIP, and the kit comprises a capture reagent, a detection buffer solution and test paper, wherein the capture reagent is biotinylated coupled lamprey immune protein LIP; the detection buffer solution is prepared from 50mM of Tris-base, 0.15M of NaCl, 0.01% of Tween-20 and 7.5% of BSA; the test paper is provided with a bottom plate, a sample adding pad, a fluorescent microsphere coupling release pad, a nitrocellulose membrane and a water absorption pad are sequentially and fixedly connected to the bottom plate from left to right in a step shape, and the fluorescent microsphere coupling release pad is prepared by spraying coupling liquid of europium fluorescent nanoparticles, streptavidin SA and rabbit IgG on polyester fibers; the nitrocellulose membrane is sequentially provided with a detection band formed by UMOD monoclonal antibodies and a reference band formed by goat anti-rabbit IgG from left to right.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a kit for detecting urothelial carcinoma based on the Neu5Gc that recognizes UMOD modified in urine by LIP. Background technique [0002] Urothelial carcinoma is one of the major diseases that seriously threaten human life and health. "Early detection, early diagnosis, and early treatment" is an important development direction and the most effective means for the diagnosis and treatment of urothelial carcinoma, which can significantly improve the survival rate of patients. Urothelial cancer includes renal pelvis cancer, bladder cancer, ureteral cancer, etc. Currently, commonly used clinical examination methods include urine exfoliation cytology examination and imaging examination. The main marker of urine exfoliation cytology examination is Neu5Gc (N-glycolylneuraminic acid), the detection principle is based on the specific reaction between sugar chain a...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/558G01N33/577G01N33/58G01N33/533G01N33/531
CPCG01N33/57407G01N33/57438G01N33/57488G01N33/558G01N33/577G01N33/582G01N33/587G01N33/531G01N33/533Y02A50/30
Inventor 逄越李庆伟滕洪明
Owner LIAONING NORMAL UNIVERSITY
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