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Preparation method and application of near-infrared excitation composite photosensitive nanoparticles capable of delivering active pharmaceutical substances

A technology of active substance and excited recombination, applied in the field of nano-biomedical materials

Active Publication Date: 2021-05-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is different from the traditional encapsulation method of loading photosensitizers into nanocarriers. It can not only overcome the problems of poor skin penetration of the light source in traditional photodynamic therapy, but also achieve rapid and near-precise release of small immunomodulatory molecules. , in addition, its preparation method is simple in process, low in cost, easy to popularize and apply, and provides a new idea for realizing efficient cancer photoimmunotherapy

Method used

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  • Preparation method and application of near-infrared excitation composite photosensitive nanoparticles capable of delivering active pharmaceutical substances
  • Preparation method and application of near-infrared excitation composite photosensitive nanoparticles capable of delivering active pharmaceutical substances
  • Preparation method and application of near-infrared excitation composite photosensitive nanoparticles capable of delivering active pharmaceutical substances

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Embodiment 1

[0038] attached figure 1 A schematic diagram showing the preparation and application of near-infrared-excited composite photosensitive nanoparticles that can deliver pharmaceutically active substances in the present invention, the steps of the preparation method and related evaluations are as follows:

[0039] 1) Synthesis of up-conversion nanoparticles (UCNPs)

[0040] According to the literature method, UCNP (NaYF 4 :Yb,Tm). YCl 3 (0.747mmol), YbCl 3 (0.25mmol) and TmCl 3 (0.003 mmol) was dispersed in 6 mL of oleic acid and 15 mL of 1-octadecene (ODE), and the above mixture was heated at 150 °C for 30 min. After forming a homogeneous solution, the mixture was cooled to room temperature, and then a solution containing NaOH and NH 4 F in methanol solution. Increase the temperature to 110°C and continue heating to evaporate the methanol, then heat to 300°C under an inert gas atmosphere and keep the temperature for 1 hour. The resulting product was washed three times wit...

Embodiment 2

[0053] A method for preparing near-infrared-excited composite photosensitive nanoparticles capable of delivering pharmaceutically active substances, the method steps and related evaluations are as follows:

[0054] 1) Synthesis of up-conversion nanoparticles (UCNPs)

[0055] According to the literature method, UCNP (NaYF 4 :Yb,Tm). YCl 3 (0.747mmol), YbCl 3 (0.25mmol) and TmCl 3 (0.003 mmol) was dispersed in 6 mL of oleic acid and 15 mL of 1-octadecene (ODE), and the above mixture was heated at 150 °C for 30 min. After forming a homogeneous solution, the mixture was cooled to room temperature, and then a solution containing NaOH and NH 4 F in methanol solution. Increase the temperature to 110°C and continue heating to evaporate the methanol, then heat to 295°C under an inert gas atmosphere and keep the temperature for 1.5 hours. The resulting product was washed three times with acetone and cyclohexane, and finally dispersed in tetrahydrofuran (THF).

[0056] 2) Synthes...

Embodiment 3

[0068] A method for preparing near-infrared-excited composite photosensitive nanoparticles capable of delivering pharmaceutically active substances, the method steps and related evaluations are as follows:

[0069] 1) Synthesis of up-conversion nanoparticles (UCNPs)

[0070] UCNP(NaYF4:Yb,Tm) was synthesized by using oleic acid as a stabilizer according to the literature method. YCl 3 (1.494mmol), YbCl 3 (0.5mmol) and TmCl 3 (0.006 mmol) was dispersed in 12 mL of oleic acid and 30 mL of 1-octadecene (ODE), and the above mixture was heated at 150 °C for 30 min. After forming a homogeneous solution, the mixture was cooled to room temperature and then added to a methanolic solution containing NaOH and NH4F. Increase the temperature to 110°C and continue heating to evaporate the methanol, then heat to 300°C under an inert gas atmosphere and keep the temperature for 1 hour. The resulting product was washed three times with acetone and cyclohexane, and finally dispersed in tetr...

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Abstract

The invention relates to a preparation method and application of near-infrared excitation composite photosensitive nanoparticles capable of delivering active pharmaceutical substances. The composite photosensitive nanoparticles are each formed by coating an up-conversion nanoparticle and a photosensitive inner core, with the aggregation-induced emission characteristic, with a pH-responsive polymer shell layer through a covalent bond, can induce generation of a large amount of reactive oxygen species (ROS) under 980nm laser irradiation to kill tumor cells, meanwhile, triggers protonation of a tertiary amine group (PC7A) in the polymer shell layer in an acidic environment, and induces the polymer to swell and releases the active pharmaceutical substances of the medicine, so that the efficiency of photodynamic therapy (PDT) is further improved. The preparation method provided by the invention is simple and non-toxic, can be used as a novel strategy to overcome the limitations of poor tissue permeability, low efficiency, immune tolerance and the like related to conventional PDT, can deliver active pharmaceutical substances of different drugs according to specific application conditions, and provides a possible platform for developing efficient and accurate light combination therapy.

Description

technical field [0001] The invention belongs to the field of nano-biomedical materials, and relates to a preparation method and application of an immunoregulatory nano-composite photosensitizer excited by near-infrared light. Background technique [0002] Tumor immunotherapy aims to regulate the immune system to recognize and eliminate tumor cells and achieve the purpose of treating tumors. In recent years, photodynamic therapy has attracted much attention due to its potential to ablate tumors and activate anti-tumor immune responses. However, the overall efficacy of photodynamic therapy is limited by immune tolerance and immunosuppression in the tumor microenvironment, often requiring additional therapeutic interventions to enhance antitumor immune responses. Conventional photosensitizers (such as peptide cyanine, porphyrin, etc.) used in traditional photodynamic therapy have a hydrophobic rigid planar structure, and are prone to aggregation through π-π stacking in aqueous...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K41/00A61K45/00A61K31/635A61P35/00
CPCA61K47/6933A61K41/0057A61K45/00A61K31/635A61P35/00A61K2300/00
Inventor 刘阳郑雅丹
Owner NANKAI UNIV
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