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Application of bacillus cereus B-28 in aspect of repairing environment polluted by heavy metal Cr

A technology of Bacillus cereus and B-28, applied in the fields of application, chemical instruments and methods, botany equipment and methods, to achieve the effect of promoting the increase of chlorophyll content of plant leaves, increasing biomass, and promoting plant growth

Inactive Publication Date: 2021-05-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese invention patent CN105925507B provides a Bacillus cereus B19 that has the functions of passivating heavy metals and promoting plant growth. and Bacillus cereus that promote plant growth have not been reported

Method used

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  • Application of bacillus cereus B-28 in aspect of repairing environment polluted by heavy metal Cr
  • Application of bacillus cereus B-28 in aspect of repairing environment polluted by heavy metal Cr
  • Application of bacillus cereus B-28 in aspect of repairing environment polluted by heavy metal Cr

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The green fluorescent protein gene transduction of test strain of embodiment 1

[0045] 1. Experimental method

[0046] (1) Plasmid DNA extraction and plasmid desalting

[0047] The pNW33 plasmid was extracted from Escherichia coli containing the pNW33 plasmid using the SanPrep column plasmid DNA mini-extraction kit (Shanghai Sangon Bioengineering Co., Ltd.), and the steps of plasmid extraction were referred to the kit instructions.

[0048] 0.1mol -1Melt glucose and 1% agarose in water Take 1mL to a 1.5mL microcentrifuge tube, then insert a 0.5mL microcentrifuge tube into a 1.5mL microcentrifuge tube. After solidification, remove the 0.5 mL microcentrifuge tube, then transfer the plasmid DNA sample to a desalting cone and desalt on ice for 2 h. Finally, the plasmid DNA was collected and the concentration of the plasmid DNA was measured using a micro-volume ultraviolet-visible spectrophotometer (ThermoFisher NanoDropOne) and stored at 4°C.

[0049] (2) Competent pre...

Embodiment 2

[0056] Example 2 Determination of bacterial strain B-28 colonization status in corn roots

[0057] 1. Experimental method

[0058] In this study, soil pot experiments were conducted to study the colonization of the strains in maize roots and their effects on maize growth and Cr uptake under different inoculation frequencies. The inoculation is inoculated with the B-28 strain transduced with the pNW33N plasmid in Example 1.

[0059] (1) Soil pot experiment design

[0060] There are 8 treatments in the soil pot test, which are: M0 (no inoculation, planting in sterilized soil); M1 (inoculation once every 7 days; planting in sterilized soil); M2 (inoculation once every 30 days; planting in sterilized soil) ); M3 (inoculation once every 60 days; planting in sterilized soil); Y0 (no inoculation, planting in non-sterile soil); Y1 (inoculation once every 7 days; planting in non-sterile soil); Y2 (inoculation every 30 days Bacteria once; plant in non-sterile soil); Y3 (inoculate onc...

Embodiment 3

[0067] Example 3 Effect of Inoculation on Chlorophyll Content and Antioxidative Enzyme Activity of Maize

[0068] 1, cultivation and inoculation method are the same as the soil pot experiment design method among the embodiment 2.

[0069] 2. Experimental results

[0070] Table 1 shows the effects of different treatments on the chlorophyll content of maize leaves and the activities of antioxidant enzymes in roots under soil pot experiments. Among them, M0, M1, M2 and M3 respectively represent the treatments of no inoculation, inoculation every 7 days, inoculation every 30 days and inoculation every 60 days in the sterilization soil treatment. Y0, Y1, Y2 and Y3 respectively represent the treatments of no inoculation, inoculation every 7 days, inoculation every 30 days and inoculation every 60 days in the non-sterile soil treatment.

[0071] Table 1 Chlorophyll content of corn leaves and antioxidant enzyme activity in roots of soil culture experiment

[0072]

[0073] Diffe...

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Abstract

The invention provides application of bacillus cereus B-28 in the aspect of repairing the environment polluted by heavy metal Cr. The content of ammonium nitrate extraction state Cr and DTPA extraction state Cr of plant rhizosphere can be remarkably reduced by inoculating a B-28 strain to a plant, the content of Cr in each tissue of the plant is remarkably reduced, the chlorophyll content in plant leaves is promoted to be increased, the activity of CAT and POD enzymes at the root is promoted to be enhanced, the biomass of each tissue of the plant is remarkably improved, and a new scheme for plant-microorganism combined passivation of Cr to promote plant growth is provided.

Description

technical field [0001] The invention relates to the technical field of bioremediation of heavy metal pollution, and more specifically relates to the application of bacillus cereus B-28 in remediating heavy metal Cr polluted environments. Background technique [0002] Due to its high mobility and high biological toxicity, chromium (Cr) has caused continuous pollution to farmland soil in my country and seriously threatened the safety of agricultural products. Among the many soil chromium pollution treatment technologies, the plant-microbe combined passivation technology is a promising direction, which refers to the reduction and stabilization of heavy metals in the soil through absorption and adsorption of plant roots, complexation of root exudates, etc., and at the same time inoculation of microorganisms It can not only assist in the passivation of heavy metals in the soil, but also enhance the tolerance of plants to heavy metals, and the root exudates released by plants can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14A01N63/22A01P21/00
CPCA01N63/22C09K17/14
Inventor 李取生阮翔李万里范瀚允侯思颖徐子杰
Owner JINAN UNIVERSITY
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