Application of lactobacillus plantarum and/or metabolite thereof in preparation of weight-reducing composition
A technology of Lactobacillus plantarum and metabolites, applied in the field of Lactobacillus plantarum, can solve problems such as overweight, achieve the effects of reducing fat accumulation, improving gene expression, and inhibiting the activity of α-glucosidase
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example 1
[0052] Example 1: Identification of strains
[0053] First, strains isolated from the fruit of the acai berry (Euterpe oleracea whole fruit) were subjected to bacterial species identification. The 16S ribosomal gene (16SrDNA) sequence (ie, SEQ ID NO: 1) of the isolated strain was obtained by polymerase chain reaction (PCR). Next, after comparing the sequence of SEQID NO: 1 with the 16S ribosomal gene (16SrDNA) of other Lactobacillus plantarum (as shown in Table 1) on the website of the National Center for Biotechnology Information (NCBI) in the United States, it can be known that the isolated strain The similarity between the 16SrDNA sequence and other Lactobacillus plantarum was 96.49% (as shown in Table 1). Therefore, this isolated strain was named Lactobacillus plantarum TCI405.
[0054] Table I
[0055]
example 2
[0056] Example 2: Preservation and cultivation experiment of Lactobacillus plantarum TCI405
[0057] First, the isolated Lactobacillus plantarum TCI405 was cultured using a liquid medium (MRS) to obtain a bacterial liquid, and then the bacterial liquid was mixed with glycerol at a ratio of 4:1. Afterwards, the mixture of bacterial liquid and glycerin was stored at -80°C.
[0058] Next, Lactobacillus plantarum TCI405 was planted with 1% bacterial growth (about 1×10 4 CFU / mL) inoculated in MRS medium (BD Difco TM Lactobacilli MRS Broth, 1% (v / v)), and cultured at 37°C under anaerobic environment for 18 hours to form TCI405 bacterial liquid.
[0059] Centrifuge the Lactobacillus plantarum TCI405 bacterial liquid at 5000rpm for 5 minutes to obtain the supernatant, and filter the supernatant with a 0.2 μm filter membrane, and the obtained filtrate is the TCI405 sample (that is, the TCI405 sample contains the metabolites of Lactobacillus plantarum TCI405 ).
example 3
[0060] Example 3: α-amylase activity test
[0061] 3-1. Solution configuration
[0062] 0.02 Molar concentration (M) sodium phosphate buffer solution (Sodium phosphate buffer) containing 6mM sodium chloride (hereinafter referred to as NaCl-Pi buffer solution): 0.7356 grams of sodium monohydrogen phosphate (purchased from J.T.Baker, No. 3828-01 ), 0.5492 grams of sodium dihydrogen phosphate (purchased from Sigma, No. 04270) and 1.7532 grams of sodium chloride (purchased from First Chemical Industry, No. C4B07) are mixed and dissolved in 500 milliliters of water (H O) to configure the 6mM NaCl-Pi buffer solution with pH 6.3.
[0063] 2 normality (N) sodium hydroxide (NaOH) solution: 8 g of sodium hydroxide (purchased from Macron, No. 7708-10) was dissolved in 100 ml of water to prepare a 2N sodium hydroxide solution.
[0064] Dinitrosalicylic acid color reagent (Dinitrosalicylic acid color reagent, hereinafter referred to as terminator): Dissolve 1 g of 3,5-dinitrosalicylic ac...
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