Mouse lung organ culture method

A technology of organ culture and organoids, applied in the field of cell biology, can solve the problems of low formation rate of lung organoids, difficult manipulation and cumbersome culture process, etc., shorten the in vitro culture period, shorten the culture period, and simplify the experimental steps Effect
CN112852709AActive Publication Date: 2021-05-28NANJING MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING MEDICAL UNIV
Publication Date
2021-05-28

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Abstract

The invention discloses a mouse lung organ culture method, which comprises the following steps: using 50-80 [mu] m mouse lung tissue primary epithelial stem cell spheres for mouse lung organ culture, adding matrigel according to the number of lung spheres, incubating a mixture of the lung spheres and Matrigel basement membrane matrigel to cure the matrigel, and culturing by using a lung organ culture medium. Two different forms of lung organs can be simultaneously differentiated and formed under the same culture system.
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Description

technical field

[0001] The invention belongs to the technical field of cell biology, in particular to a method for culturing mouse lung organoids. Background technique

[0002] At present, monolayer adherent cell culture on two-dimensional (2D) substrates (such as polystyrene or glass) has become the main research method of traditional cell culture systems, but the morphological characteristics, growth patterns, and physiological functions of cells in 2D culture There are obvious differences with the real environment in the body, and the physiological correlation is not strong. In recent years, the development of a large number of 3D cell culture models has significantly promoted the research and development of tumor biology, tissue engineering, and regenerative medicine. The 3D cell culture model can self-assemble into microspheres through suspension culture or induce differentiation to form organoids based on biological scaffolds (such as extracellular matrix). Cell-cell ...

Claims

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