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Preparation method and application of photocatalyst based on arginine aptamer

A photocatalyst and aptamer technology, applied in the field of biomedicine, can solve the problems of binding interference and the inability of the reaction to occur, and achieve the effect of easy availability of raw materials and simple method.

Active Publication Date: 2021-06-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of a large number of organic molecules in the physiological environment, the binding of carbon quantum dot-doped graphitic carbon nitride to arginine will be significantly disturbed, making the reaction almost impossible to occur.

Method used

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  • Preparation method and application of photocatalyst based on arginine aptamer
  • Preparation method and application of photocatalyst based on arginine aptamer
  • Preparation method and application of photocatalyst based on arginine aptamer

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preparation example Construction

[0020]A method of preparation of a photocatalyst based on an arginine adapter comprises the steps of:

[0021](1) Preparation of carbon quantum dot solution: dissolve citric acid and urea in mass ratio 4: 1 in secondary distilled water, placed in a polytetrafluoroethylene reaction kettle, 160-200 ° C high temperature reaction 16-20h, then Cool to room temperature, remove the precipitate at a rotational speed of ≥ 10000 rpm, and then place the supernatant into the dialysis bag in the dialysis of 3-7 days, then rotate under 40-60 ° C, the vacuum is 25-50 mbar conditions, and the evaporation is obtained. Mg · L-1Carbon quantum dot solution;

[0022](2) Preparation of carbon quantum doped graphite phase breened carbon: dissolved urea in the carbon quantum point solution prepared by step (1), and then placed in a crucible, and heated at 500-600 ° C for 2-4h After the cooling was filtered, the resulting product was ground into powder and placed in a nitric acid solution of 5 mol / L, and the pr...

Embodiment

[0029](1) 2 g of citric acid and 0.5 g of urea were dissolved in 25 ml of secondary distilled water, placed in a polytetrafluoroethylene reaction kettle, warmed to 170 ° C, heat insulation reaction 18 h, then cooled to room temperature, and the product was 10000 The RPM was centrifuged for 10 min, and the resulting supernatant was placed in a dialysis bag of 10 kDa in a dialysis bag of 10 kDa, and then the resulting dialysate was rotated at 40 ° C, vacuum degree of 25 mbar, and was prepared to obtain a carbon quantum point. Solution, its concentration is 2 mg · L-1;

[0030](2) 10 g of urea is dissolved in 5 mL of the carbon quantum point solution prepared above, and then placed in a crucible, and at 5 ° C min-1The speed is warmed to 550 ° C, the reaction reaction is 3 h, then cooled to room temperature, and the product is ground into powder and placed in 5 MHNO.3In the solution, the temperature was maintained at 130 ° C, refluxed 24 h, centrifugation after cooling to room temperature ...

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Abstract

The invention discloses a preparation method and application of a photocatalyst based on an arginine aptamer, and the photocatalyst based on the arginine aptamer is formed by taking an amino-modified arginine aptamer and carbon quantum dot doped graphite phase carbon nitride as raw materials and enabling the amino-modified arginine aptamer and the carbon quantum dot doped graphite phase carbon nitride to be subjected to an amide condensation reaction. The preparation method disclosed by the invention is simple, green, mild and low in cost, and the obtained photocatalyst can enrich arginine in cells around through the linked aptamer, and generates photogenerated holes under the excitation of red light with the wavelength of more than 630 nm to catalyze the enriched arginine around to generate nitric oxide. The glycolysis and oxidative phosphorylation of tumor cells can be respectively inhibited by the consumption of arginine and the generation of nitric oxide in the cells, so that the tumor cells can be killed through the synergistic effect of the two ways, and a new technical support is expected to be provided for the synergistic treatment of cancers.

Description

Technical field[0001]The present invention belongs to the field of biomedical, and more particularly to an alternanine-based photocatalyst based on tumor treatment and a preparation method thereof.Background technique[0002]The sufficient energy supply is the premise of cancer cells are not controlled, infiltrated, and metastasis, and therefore, it is a valid strategy for glycolysis and phosphorylation processes that provide energy to cancer cells to provide energy. However, the powerful metabolic plasticity of cancer cells can adjust its metabolic form between glycolysis and phosphorylation to accommodate energy lack of energy, resulting in a single inhibitor. In contrast, inhibitors using glycolysis and oxide phosphorylation have a broader prospects in eliminating tumors. However, due to the inherent pharmacokinetic differences in various drugs, co-inhibition of glycolysis and phosphorylation is still a huge challenge in time and space.[0003]During various physiological processes o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/06A61K47/54A61K41/00A61P35/00
CPCB01J31/069A61K41/0057A61K47/549A61P35/00B01J35/39
Inventor 卢春华方啸袁萌杨黄浩
Owner FUZHOU UNIV
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