Chiral cysteine nano self-assembled antibacterial material, preparation method and application thereof

A nano-self-assembly and cysteine ​​technology, which is applied in the field of chiral cysteine ​​nano-self-assembly antibacterial materials and its preparation, can solve the problems of high preparation cost, instability, and impact on industrial application

Active Publication Date: 2021-06-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its industrial application is seriously affected by problems such as high preparation cost, low solubility, and instability.

Method used

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  • Chiral cysteine nano self-assembled antibacterial material, preparation method and application thereof
  • Chiral cysteine nano self-assembled antibacterial material, preparation method and application thereof
  • Chiral cysteine nano self-assembled antibacterial material, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of Au / D-cys nanoparticles:

[0029] Take a clean Erlenmeyer flask and place it on a stirring heater, add 48.75mL ultrapure water and 1.25mL 10mmol / L chloroauric acid solution in turn, stir and heat to boiling, then quickly add 1mL 1.2% trisodium citrate solution, After the color of the solution turned into wine red, stop heating, add 1mL 150mmol / L sodium hydroxide solution and 1mL 200mmol / L D-cys solution in turn, and stir at room temperature for 2h to obtain Au / D-cys nanoparticle solution; Au / D- The particle size of cys nanoparticles is 15±2nm; the molar concentration of Au / D-cys in Au / D-cys nanoparticle solution is 5nmol / L.

[0030] (2) Preparation of Au / D-cys-PEG nanoparticles:

[0031] Add 20 μL of 6 mM mercaptopolyethylene glycol solution (molecular weight: 1000 kD) to 1 mL of the Au / D-cys nanoparticle solution obtained in step (1), stir at room temperature for 1 h, centrifuge at 7200 rpm / min for 25 min, remove the supernatant, add supernatant The...

Embodiment 2

[0035] (1) Preparation of Au / L-cys nanoparticles:

[0036] Take a clean Erlenmeyer flask and place it on a stirring heater, add 48.75mL ultrapure water and 1.25mL 10mmol / L chloroauric acid solution in turn, stir and heat to boiling, then quickly add 1mL 1.2% trisodium citrate solution, After the solution turned into wine red, stop heating, add 1mL 150mmol / L sodium hydroxide solution and 1mL 200mmol / L L-cys solution in turn, and stir at room temperature for 2h to obtain Au / L-cys solution; Au / L-cys nano The particle size of the particles is 10±2nm; the molar concentration of Au / L-cys in the Au / L-cys nanoparticle solution is 5nmol / L;

[0037] (2) Preparation of Au / L-cys-PEG nanoparticles:

[0038] Add 20 μL of 6mM mercaptopolyethylene glycol solution (molecular weight: 1000kD) to the 1mL Au / L-cys nanoparticle solution obtained in step (1), stir at room temperature for 1h, centrifuge at 7200rpm / min for 25min, remove the supernatant, add supernatant Resuspend in pure water to obt...

Embodiment 3

[0042] (1) Preparation of Au / D-cys nanoparticles:

[0043]Take a clean Erlenmeyer flask and place it on a stirring heater, add 48.75mL ultrapure water and 1.25mL 10mmol / L chloroauric acid solution in turn, stir and heat to boiling, then quickly add 1mL of 1.5% trisodium citrate solution , stop heating after the color of the solution turns wine red, add 1mL 150mmol / L sodium hydroxide solution and 1mL 250mmol / L D-cys solution in turn, stir at room temperature for 2h, and obtain Au / D-cys nanoparticle solution; Au / D The particle size of -cys nanoparticles is 10±1nm; the molar concentration of Au / D-cys in the Au / D-cys nanoparticle solution is 10nmol / L;

[0044] (2) Preparation of Au / D-cys-PEG nanoparticles:

[0045] Add 20 μL of 3mM mercaptopolyethylene glycol solution (molecular weight: 350kD) to the 1mL Au / D-cys nanoparticle solution obtained in step (1), stir at room temperature for 1h, centrifuge at 7200rpm / min for 25min, remove the supernatant, add supernatant Resuspend in p...

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Abstract

The invention discloses a chiral cysteine nano self-assembled antibacterial material, a preparation method and application thereof, and belongs to the technical field of nano materials. According to the method, nano-gold is taken as a core, a small amount of sulfydryl polyethylene glycol is taken as a stabilizer, cysteine is fixed on the surface of the nano-gold through Au-S bonds in a fractional adding manner, and the chiral cysteine nano self-assembled antibacterial material is prepared. According to the method disclosed by the invention, the stability of nano-gold and simple cysteine can be remarkably improved, and the broad-spectrum antibacterial property on pathogenic bacteria such as escherichia coli and staphylococcus aureus is shown.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a chiral cysteine ​​nanometer self-assembled antibacterial material and its preparation method and application. Background technique [0002] L-cysteine ​​(L-Cys) is a common amino acid in the body, which can eliminate inflammation, accelerate the decomposition and excretion of heavy metal ions in the body, reduce the accumulation of acetaldehyde in the body, and protect the liver. At present, it has been used in food, medicine, cosmetics and other fields. D-cysteine ​​(D-Cys) is the natural enantiomer of L-Cys, which is mainly synthesized by L-Cys chiral catalysis, and has a certain antibacterial effect on most bacteria such as Escherichia coli and Staphylococcus aureus , especially for Escherichia coli has a strong antibacterial effect. However, its industrial application is seriously affected by problems such as high preparation cost, low solubility, and in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/16A01N37/44A01N25/08A01P1/00B22F9/24B82Y40/00
CPCA01N59/16A01N37/44A01N25/08B22F9/24B82Y40/00
Inventor 严文静徐跃龙张敏章建浩
Owner NANJING AGRICULTURAL UNIVERSITY
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