Pharmaceutical application of hydrogen sulfide donor

A hydrogen sulfide donor, sodium hydrosulfide technology, applied in the field of biomedicine, can solve the problems of no relevant research, loss of smooth muscle, etc., and achieve the effect of reducing mortality, reducing the level of inflammatory factors, and reducing the incidence rate

Inactive Publication Date: 2021-06-04
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether hydrogen sulfide changes during the occurrence of aortic aneurysm and aortic dissection and whether it is involved in inflammatory injury and smooth muscle loss in aortic aneurysm and aortic...

Method used

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  • Pharmaceutical application of hydrogen sulfide donor
  • Pharmaceutical application of hydrogen sulfide donor
  • Pharmaceutical application of hydrogen sulfide donor

Examples

Experimental program
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Effect test

Embodiment 1

[0017] 1. RNA extraction and Q-PCR

[0018] Extraction of total RNA

[0019] (1) After the vascular tissue was washed with ice-cold PBS, 1 mL of Trizol was added, the cells were pipetted and transferred to an RNase-free Eppendorf centrifuge tube, and placed on ice for 5 minutes;

[0020] (2) Add 200 μL of chloroform, shake vigorously to mix, and place on ice for 2-3 minutes;

[0021] (3) 12000rcf, 4°C, 15 minutes;

[0022] (4) Transfer 400 μL of the supernatant to a new Eppendorf centrifuge tube, add an equal volume of isopropanol, mix by inverting, and place on ice for 10 minutes;

[0023] (5) 12000rcf, 4°C, 10 minutes, discard the supernatant;

[0024] (6) Add 75% ethanol diluted with DEPC water, 7500rcf, 4°C, 5 minutes;

[0025] (7) Take the supernatant, dry it at room temperature, add 20 μL DEPC water to dissolve, and measure the concentration of RNA with a UV spectrophotometer;

[0026] (8) Store the purified RNA samples at -80°C.

[0027] reverse transcription

[...

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Abstract

The invention provides pharmaceutical application of a hydrogen sulfide donor, particularly relates to application of hydrogen sulfide and a hydrogen sulfide donor in preparation of medicines for treating aortic aneurysm and aortic dissection, and particularly relates to application of sodium hydrosulfide, DATS, L-cysteine and analogues thereof, GYY4137, ADT-OH and analogues thereof, and Gem-dithiol and analogues thereof in preparation of medicines for treating aortic aneurysm and aortic dissection. According to the invention, through an integral animal model, the hydrogen sulfide and the hydrogen sulfide donor are determined to be capable of reducing the occurrence rate and death rate of aortic aneurysm and aortic dissection, increasing the content of hydrogen sulfide in circulating blood, inhibiting expression of inflammatory factors in the process of aortic aneurysm and aortic dissection, and improving loss of smooth muscle cells in the process of aortic aneurysm and aortic dissection. The hydrogen sulfide donor can be used as a treatment medicine for improving occurrence and development of aortic aneurysm and aortic dissection.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of hydrogen sulfide donors in the preparation of medicines for treating aortic aneurysm and aortic dissection. Background technique [0002] Aortic aneurysm (AoA) and aortic dissection (AoD) are multifactorially induced and potentially fatal vascular diseases with a genetic predisposition. Aortic aneurysm manifests as aneurysmal dilation of the aorta, which is defined as dilation of the artery greater than 1.5 times the normal arterial diameter. Aortic dissection is manifested by tearing of the intima of the aortic wall, and circulating blood enters the media of the aortic wall through the intima breach, resulting in a large-scale tear of the media and the formation of a dissection. The etiology of more than 80% of patients with AoA and AoD is hypertension. According to statistics, AoA and AoD account for 1%-3% of the adult male mortality of 65-85...

Claims

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Application Information

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IPC IPC(8): A61K33/04A61K31/105A61K31/198A61K31/5375A61K31/385A61K31/095A61P35/00A61P9/00A61P9/14
CPCA61K33/04A61K31/105A61K31/198A61K31/5375A61K31/385A61K31/095A61P35/00A61P9/00A61P9/14
Inventor 季勇罗姗姗周雪纯孔垂玉
Owner NANJING MEDICAL UNIV
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