Preparation method of immunogen-removed human umbilical cord intima-fibrin composite skin scaffold

Abstract

The invention discloses a preparation method of an immunogen-removed human umbilical cord intima-fibrin composite skin scaffold, and belongs to the technical field of artificial skin.The preparation method comprises the following steps: taking a placenta without virus or bacterial infection, cutting off an umbilical cord, passively stripping the umbilical cord from the umbilical cord basal layer under a sterile condition to obtain an umbilical cord intima by a non-invasive expansion method such as sterile normal saline, air, nitrogen and the like. and removing impurities on the surface of the umbilical cord intima; cleaning the stripped umbilical cord intima with normal saline, and placing the umbilical cord intima in a self-prepared culture solution containing antibiotics, performing soaking and disinfecting, and filtering subpackaging the soaking solution, and storing the soaking solution at 80 DEG C below zero for later use; and dehydrating the umbilical cord intima by using organic solutions-normal saline with different concentrations. According to the preparation method, the skin scaffold attached to the wound is active, so that the skin scaffold can be integrated with the human skin within a period of time after being attached to the wound, and the wound can be permanently repaired.

Description

Technical field [0001] The present invention relates to the field of artificial skin technologies, and specific to the immunogenic human umbilical inner membrane-fibrin composite skin bracket. Background technique [0002] The matrix layer umbilical endometrium is a thin film matrix composed of single-layer epithelial cells. With the growth of embryos, the inner membranes are attached to the umbilical cord matrix layer, and toughness. The umbilical cord inner film is the innermost surface of the umbilical cord, no blood, no nerve and lymph, having a certain elasticity, thickness of about 0.1-0.2mm, the inner film containing the glue, fiber adhesion protein, layer adhesion protein, is exact These ingredients enable the umbilical cord inline to act as a "transplanted base film" to promote new healthy and suitable matrix effects to promote epithelialization. With promoting epidemic adhesion growth and proliferation, reducing inflammation, suppressing new blood vessel formation, redu...

AI Technical Summary

Problems solved by technology

[0003] Currently, skin grafting is performed on the affected part of the human body, most of which use autologous skin for transplantation, but when the wound area is large, the transfer of autologous skin is limited, which cannot meet medical needs, and because autologous skin transplantation requires surgery to Realized, this will increase the pain of the patient during the treatment period, which is not conducive to the healing of the patient's wound. In addition, although there are skin products that have appeared on the market and are used in clinical practice, due to their lack of ductility, artificially synthesized skin scaffolds After the cutting size is fixed, it can only be applied to a certain size of the affected area, which will affect the scope of application of the composite skin support

Images