Rapid quantitative detection card for Abrin toxin

Abstract

The invention discloses a rapid quantitative detection card for Abrin toxin. The rapid quantitative detection card comprises a PVC (polyvinyl chloride) bottom plate, a water absorption pad, a nitrocellulose membrane, a marker pad, a sample pad and a diluent pad which are arranged in a shell, the nitrocellulose membrane is fixed on the PVC bottom plate, the left end of the nitrocellulose membrane is in lap joint with the water absorption pad, the right end of the nitrocellulose membrane is in lap joint with the dilution pad, the marker pad is arranged on the right side of the middle of the nitrocellulose membrane, the sample pad is arranged on the marker pad, and the quality control line and the detection line are arranged between the water absorption pad and the marker pad on the nitrocellulose membrane; window holes corresponding to the detection line and the quality control line are formed in the upper shell of the shell, quantitative identification lines are arranged on the detection line and the edge of the window hole close to the marker pad, a sample adding hole is formed corresponding to the sample pad, and a cleaning hole is formed corresponding to the dilution pad. According to the invention, a chromatographic immunoassay method of adding a sample into the sample adding hole, adding a diluent into the cleaning hole and controlling the lateral liquid flow amount of the sample through the quantitative marking line is adopted, so that the sensitivity and the precision of a detected object are improved, and the characteristics of rapidness, simplicity and convenience of a rapid diagnosis detection method are retained.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to an abrinia toxin rapid quantitative detection card. Background technique [0002] Abrin toxin (Abrin toxin, AT) is a toxin protein extracted from the seeds of the legume vine plant Abrus precatorius, its molecular structure and poisoning mechanism are very similar to ricin toxin (RT), The relative molecular weight is between 63 and 67kDa, with A-B chain structure characteristics, belonging to type II ribosome inactivation protein, and is a highly toxic protein. The LD50 of AT mice is 0.04 μg / kg, the lethal dose to humans is 0.1-1 μg / kg, and the lethal dose ingested is 5.0-7.0 μg / kg, which is ricin (mouse LD50 3.0 μg / kg) more than 70 times. Due to its strong toxicity, wide sources, and large amount of preparation under relatively low technical conditions, AT is one of the only two plant toxins in the biological weapons check list, and it is also one of the importa...

AI Technical Summary

Problems solved by technology

[0003] However, since abrin toxin has no specific chemical group, it is difficult to identify it by physical and biochemical methods, so it can only rely on immunological detection methods

Among them, the ELISA method is the most classic method in the world, and its sensitivity can reach ng / mL, but its application is relatively limited due to the complicated operation, large volume of detection equipment, and long time consumption; Depending on the nature of positioning, it gets rid of the limitation of special instruments and equipment, but it can only do semi-quantitative detection, and the sensitivity is also low

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