Preparation method of canna edulis RS3 resistant starch and application thereof in functional foods and anti-Parkinson drugs
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A technology of resistant starch and canna, which is applied in canna RS3 resistant starch and its application in functional food and anti-Parkinson drugs, can solve the problem of less research
Active Publication Date: 2021-06-08
王学勇
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[0048] The invention discloses a preparation method of canna RS3 resistant starch, such as figure 1 shown, including the following steps:
[0049] Step 1. Canna taro natural starch is placed in phosphate buffer solution, and gelatinizes completely in boiling water;
[0050] Step 2. Add 0.1-5U / g α-amylase to the gelatinized starch in step 1, and hydrolyze for 1-15min;
[0051] Step 3. Add 0.1-4ASPU / g pullulanase to step 2, and hydrolyze for 1-8h;
[0052] Step 4. The starch obtained in step 3 is cooled to room temperature and stored at 4° C. for 12 hours to regenerate;
[0053] Step 5. Centrifuge the starch in step 4 to collect recrystallized starch, dry, grind, and filter to obtain Ce-RS3.
Embodiment 1
[0054] Embodiment 1 A kind of preparation method of canna taro RS3 resistant starch
[0055] Material:
[0056]Plantain natural starch was obtained from Guizhou Yilitai Biotechnology Co., Ltd. Pullulanase (1000ASPU / ml) was purchased from Novozymes Investment Co., Ltd. (Beijing, China). High-temperature α-amylase (10000 U / g) was purchased from Solarbio Co., Ltd. (Beijing, China). Potato amylose standard, amyl glucosidase, and porcine pancreatic alpha-amylase were provided by Sigma, Inc. (St. Louis, MO, USA). The D-glucose assay kit was obtained from Megazyme International Limited (Wicklow, Ireland). Deionized water was purified using a Milli-Q system (milipore, MA, USA).
[0057] Canna RS3 preparation:
[0058] 150g canna native starch was placed in 1000ml phosphate buffer solution (pH5.0), and completely gelatinized in boiling water for 20min. After that, add high-temperature α-amylase, stir at 200 rpm, adjust the cooked starch to 60°C, adjust the pH to 5.0, and then imm...
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Abstract
The invention provides a preparation method of canna edulis RS3 resistant starch and an application thereof in functional foods and anti-Parkinson drugs. The preparation method comprises the following steps: step 1, putting natural canna edulis starch in a phosphate buffer solution, and completely gelatinizing the starch in boiling water; step 2, adding 0.1-5U/g alpha-amylase into the gelatinized starch in the step 1, and carrying out hydrolysis for 1-15 minutes; step 3, adding 0.1-4ASPU/g pullulanase into the mixture obtained in the step 2, and carrying out hydrolysis for 1-8 h; step 4, cooling the starch obtained in the step 3 to room temperature, and storing the starch at 4 DEG C for 12 hours for retrogradation; and step 5, centrifuging the starch in the step 4, collecting recrystallized starch, and conducting drying, grinding and filtering to obtain Ce-RS3. According to the technical scheme, the canna edulis RS3 type resistant starch is mainly of a B-type crystal structure, and has high crystallinity and longer polymerization degree chain compared with common RS. The unique and novel structural characteristic of the CE-RS3 ensures that the CE-RS3 has better activity for treating the Parkinson's disease.
Description
technical field [0001] The invention relates to the technical fields of functional food and medicine, in particular to canna RS3 resistant starch and its application in functional food and anti-Parkinson drugs. Background technique [0002] Parkinson's disease (PD) is a common neurodegenerative disease, and its incidence and prevalence increase with age. Most of the patients are over 60 years old. It is a chronic progressive disease with high heterogeneity. Different patients have different rates of disease progression. Currently, it cannot be cured. The prevalence of PD in people over 65 years old in my country is about 1.7%. The pathogenesis of Parkinson's disease is mainly the degeneration and death of dopamine (DA) neurons in the substantia nigra of the midbrain, which leads to a significant decrease in the content of DA in the striatum and causes the disease. The exact cause of this pathological change is currently Still unclear. [0003] At present, there are many d...
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