Synthesis of glycyrrhizic acid nanoparticles and combined treatment application of glycyrrhizic acid nanoparticles in novel coronavirus pneumonia

A technology of nanoparticles and glycyrrhizic acid, which is applied in the direction of antiviral agents, medical preparations containing active ingredients, anti-inflammatory agents, etc., can solve the problems of limited therapeutic potential, poor solubility, and low bioavailability, and achieve improved biophase Poor capacitance, low cost and simple operation

Inactive Publication Date: 2021-06-18
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the therapeutic potential of glycyrrhizic acid has been limited due to its cytotoxicity and poor solubility in water and biological fluids, resulting in low bioavailability

Method used

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  • Synthesis of glycyrrhizic acid nanoparticles and combined treatment application of glycyrrhizic acid nanoparticles in novel coronavirus pneumonia
  • Synthesis of glycyrrhizic acid nanoparticles and combined treatment application of glycyrrhizic acid nanoparticles in novel coronavirus pneumonia
  • Synthesis of glycyrrhizic acid nanoparticles and combined treatment application of glycyrrhizic acid nanoparticles in novel coronavirus pneumonia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Synthesis and Characterization of Glycyrrhizic Nanoparticles (GANPS)

[0031] When the glycyrrhizic nanoparticles were synthesized, glycyrrhic acid was used as a carbon source, and the pH was adjusted to 10.0 ± 0.5 using a saturated sodium hydroxide solution, followed by incubation at 180 ° C for 7 h.

[0032] The synthesis of glycyrrhizic nanoparticles mainly includes the following steps:

[0033] A, 0.10 g (0.12 mmol) glycyrrhizic acid dissolved in 10 ml deionized water;

[0034] B, then a drop of saturated a saturated sodium hydroxide solution was adjusted to 10.0 ± 0.5;

[0035] C, poured the mixture into a 25 mL of the polytetrafluoroethylene lining, incubation for 7 h at 180 ° C;

[0036] D. After nature is cooled to room temperature, 10 000 rpm is centrifuged for 10 min from the mixture from the mixture;

[0037] E, subsequently collecting the upper cleaner, and the small precipitate is further removed by filtration by 0.22 μm filter;

[0038] f, Finally, u...

Embodiment 2

[0042] Example 2Ganps of biocompatibility assessment

[0043] Board, 96-well plate (generally in flat), the number of cells is 5000 tell / well, 100 ul medium per hole; formulated different material concentration gradient (0 mg / ml, 0.2 mg / ml, 0.4 mg / ml, 0.6 mg) / ml, 0.8 mg / ml, 1.2 mg / ml) (6 times each set of concentration gradients); each hole corresponds to 100 ul, one vertical row, one vertical row. (From left to right, low concentration to high concentration); 37 ° C overnight incubation 24h; configure CCK8 solution, more equipped with a hole (43 holes), 430 ul of CCK8 solution to add 4.000 ml of medium; discard the original cultures in the hole Base, each hole is added to 100 ul (first control group, and then the material concentration is low to high plus, longitudinally sampled), and the ultraviolet absorption peak was measured at 37 ° C, and the ultraviolet absorption peak was measured every 10 minutes. The experiment can be terminated when the control group OD is ...

Embodiment 3

[0050] Example 3GANPS for in vitro treatment effects of SARS-COV-2 infected cells

[0051] Briefly, L929 cells were incubated for 2 hours in DMEM (containing 2% fetal bovine serum) and 0.40 mg / ml Ganps, and viruses were incubated at 4 ° C and 0.40 mg / ml Ganps for 1 hour. Then, the medium containing Ganps was removed, and the pretreated MOI was replaced by a virus 1. After incubating the incubator for 1 h, discard it, washed twice with DMEM, and then incubated with different doses of Ganps for 24 h. The inhibitory effect of Ganps on viral infection was evaluated with a mild spot method and RT-QPCR. The supernatant was collected at a different point in time, and then fresh medium was added to the cells, and the cells were stored at -80 ° C; after freezing, the sample was collected as a cell lysate. All collected samples are stored at -80 ° C and quantify the number of virals in the sample through air spots.

[0052] The number of viral particles was detected with a vacuum test, ...

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Abstract

The invention belongs to the technical field of biology and discloses a method for synthesizing glycyrrhizic-acid-based nanoparticles (GANPs) through a hydrothermal method and application of the GANPs in anti-inflammatory and antiviral combined action on COVID-19. According to the method, the nanoparticles are synthesized through the hydrothermal method based on an active ingredient (glycyrrhizic acid) of Chinese herbal medicines. The method is simple in principle, rapid in method, simple in operation, low in cost and easy to popularize; and the synthesized nanoparticles play a role in improving biocompatibility of a raw material glycyrrhizic acid and meanwhile have very good antiviral and anti-inflammatory combined treatment actions on the COVID-19. According to the method, the nanoparticles are applicable to relieving of excessive inflammatory reactions caused by SARS-CoV-2, so that a nanoparticle prodrug with very good antiviral and anti-inflammatory combined treatment actions is provided for the COVID-19 based on the existing technologies.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and more particularly, the synthesis of glycyrrhizic acid nanoparticles and its combined treatment in new coronary virus pneumonia. Background technique [0002] New coronavirus pneumonia, referred to as "new coronal pneumonia" (COVID-19) is an acute respiratory infectious disease caused by new coronavirus infections. New crown pneumonia has created extensive onset and death in the world, which has a considerable impact on global economic and health. Serious COVID-19 patients hyperflammation can lead to acute respiratory distress syndrome (ARDS) and multi-organ failure, leading to death. However, current management is supportability, no specific drug confrontation COVID-19. Therefore, there is an urgent need for a safe and effective treatment strategy to inhibit the severe acute respiratory syndrome 2 coronavirus (SARS-COV-2) while alleviating COVID-19 out of control. [0003] Nanoparticles are wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/704A61P31/14A61P11/00A61P29/00
CPCA61K9/14A61K31/704A61P31/14A61P11/00A61P29/00
Inventor 黄曦赵钊艳肖雨晨
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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