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Preparation method of flavane component extract in lacquer tree, product and application thereof

A technology of flavans and extracts, which is applied in the field of preparation of flavans extracts from sumac, can solve problems such as mitochondrial dysfunction, increase of free radicals, and death of nerve cells, so as to improve development, high-value utilization, and ease Effects of treatment scarcity

Inactive Publication Date: 2021-06-18
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the excitatory amino acids in the body increase greatly, far exceeding the normal level, the NMDA receptors will be overactivated, causing a large amount of Ca 2+ influx, which in turn leads to intracellular Ca 2+ Overload, which induces NO production, leads to mitochondrial dysfunction and increased free radicals, which in turn trigger neuronal cell death

Method used

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  • Preparation method of flavane component extract in lacquer tree, product and application thereof
  • Preparation method of flavane component extract in lacquer tree, product and application thereof
  • Preparation method of flavane component extract in lacquer tree, product and application thereof

Examples

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Effect test

Embodiment 1

[0027] Embodiment 1: Preparation method of extract of flavans in sumac:

[0028] (1) 25kg sumac tree trunks are dried and pulverized, passed through a 30 mesh sieve, added 250Kg of 95% ethanol, heated and condensed and refluxed for extraction 3 times, each time for 3 hours, the extracts were combined, and the solvent was recovered to obtain 1.7kg of ethanol extract;

[0029] (2) Step (1) After the ethanol extract is dispersed and dissolved in 80 L of water, it is extracted 3 times with equal volumes of petroleum ether, dichloromethane, ethyl acetate and n-butanol, and the filtrate is combined and the solvent is recovered to obtain petroleum ether extraction. Extract 70g, dichloromethane extract 70g, ethyl acetate extract 1.2kg and n-butanol extract 200g;

[0030] (3) Take step (2) ethyl acetate extract 1.2kg, carry out gradient elution with the dichloromethane-methanol eluent of volume ratio 100:0-0:100 with silica gel column chromatography, dichloromethane and methanol The r...

Embodiment 2

[0031] Embodiment 2: preparation method of extract of flavans in sumac tree:

[0032] (1) The sumac tree trunk is sieved after being dried and pulverized, added with ethanol, heated and condensed to reflux for extraction, and the solvent is recovered from the extract to obtain an ethanol extract;

[0033] (2) Step (1) gained ethanol extract is dispersed and dissolved with water, extracted 3 times with equal volumes of sherwood oil, dichloromethane, ethyl acetate and n-butanol successively, after merging the filtrate and reclaiming the solvent, sherwood oil extract, dichloromethane Methyl chloride extract, ethyl acetate extract and n-butanol extract;

[0034] (3) The ethyl acetate extract obtained in step (2) adopts silica gel column chromatography to carry out gradient elution with dichloromethane-methanol to obtain 13 fractions, according to the results of TLC thin-layer chromatography analysis, merge the fractions with consistent main spots , separated and purified by polyami...

Embodiment 3

[0035] Embodiment 3: preparation method of the extract of flavans in sumac:

[0036] (1) The sumac tree trunk is dried and crushed, passed through a 30-mesh sieve, added with 10 times the mass of 95% ethanol, heated and condensed to reflux for extraction twice, each time for 4 hours, the extracts are combined, and the solvent is recovered to obtain the ethanol extract;

[0037] (2) The ethanol extract obtained in step (1) is dispersed and dissolved by adding 45 times the weight of water, and extracted 3 times with equal volumes of sherwood oil, dichloromethane, ethyl acetate and n-butanol successively, and the filtrate is combined and the solvent is recovered to obtain Petroleum ether extract, dichloromethane extract, ethyl acetate extract and n-butanol extract;

[0038] (3) The ethyl acetate extract obtained in step (2) adopts silica gel column chromatography to carry out gradient elution with dichloromethane-methanol whose volume ratio is 100:0-0:100, and obtains 13 fraction...

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Abstract

The invention discloses a preparation method of a flavane component extract in a lacquer tree. The method includes the steps of drying, crushing and sieving lacquer tree trunks, carrying out reflux extraction with ethanol, dispersing an obtained extract by adding water, sequentially extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol, carrying out gradient elution on an obtained ethyl acetate extract through silica gel column chromatography, merging fractions with consistent main spots, after polyamide column chromatography separation and purification, carrying out ethanol-water gradient elution, combining fractions with a large amount of main spots, carrying out gel column chromatography elution and high performance liquid, and obtaining the flavanoid component extract. The invention further discloses a product and application. According to the preparation method of the flavane component extract in the lacquer tree, flavane components in the lacquer tree are enriched, so that the obtained extract has the effect of repairing damaged nervous tissues and has a protective effect on oxidative damage of H2O2-induced SH-SY5Y cells and excitatory damage induced by NMDA. The method has great significance in relieving deficiency of nervous system disease treatment drugs, and meanwhile development and high-value utilization of lacquer tree resources are improved.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a preparation method, product and application of an extract of flavans in sumac. Background technique [0002] Sumac (Toxicodendron vernicifluum (Stokes) F.A. Barkl.) is a paint of the genus Lacquer in the Anacardaceae family. The main product of the sumac tree is lacquer. In addition, the seeds of the sumac tree can be used to extract oil, the peel can be used to extract wax, the leaves can be used to extract tannins, and the leaves and roots can also be used as biological pesticides. , deworming and antitussive effects. Lacquer oil is the oil squeezed from the fruit of the sumac tree. Many ethnic minorities in my country, including the Lisu, Nu and Dulong, often use lacquer oil to make food for pregnant women. Therefore, the utilization value of sumac resources is very high, but the current utilization rate is not high. Studies have shown that flavano...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/22A61P25/00A61P39/06A61P25/18
CPCA61K36/22A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P25/00A61P25/18A61P39/06
Inventor 范艳华钟婷许永男刘建宇
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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