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Bovine serum albumin-folic acid modified chitosan nano drug delivery system loaded with berberine and doxorubicin and preparation method of bovine serum albumin-folic acid modified chitosan nano drug delivery system

A nanotechnology of bovine serum albumin and chitosan, which is applied in the field of polysaccharides, can solve the problems of general drug loading efficiency, poor solubility of chitosan, and difficulty in preparing microcapsule carriers, so as to improve drug loading efficiency, stability, The effect of preventing metastasis and recurrence

Pending Publication Date: 2021-06-22
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, chitosan has poor solubility and is not easy to prepare various microcapsule carriers
Bovine serum albumin is a commonly used protein carrier in drug delivery systems. It has many advantages such as non-toxic, degradable, non-immunogenic, and easy to administer and inject. However, its drug loading efficiency is average.

Method used

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  • Bovine serum albumin-folic acid modified chitosan nano drug delivery system loaded with berberine and doxorubicin and preparation method of bovine serum albumin-folic acid modified chitosan nano drug delivery system
  • Bovine serum albumin-folic acid modified chitosan nano drug delivery system loaded with berberine and doxorubicin and preparation method of bovine serum albumin-folic acid modified chitosan nano drug delivery system
  • Bovine serum albumin-folic acid modified chitosan nano drug delivery system loaded with berberine and doxorubicin and preparation method of bovine serum albumin-folic acid modified chitosan nano drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of folic acid modified chitosan: add 1g of folic acid to 1000g of dimethyl sulfoxide, stir, add 2g of dicyclohexylcarbodiimide and 1.2g of N-hydroxysuccinimide, stir for 0.5 hours, add 10g of chitosan For polysaccharides, heat up to 40°C, stir for 12 hours, dialyze the mixed solution for 72 hours with a dialysis bag with a molecular weight of 5000, and freeze-dry to obtain folic acid-modified chitosan;

[0029] ⑵Preparation of bovine serum albumin-folate-modified chitosan nano drug-loading system loaded with berberine and doxorubicin: take the folic acid-modified chitosan prepared in step 1 and 0.5g graphene oxide, add to 1000g In water, sonicate for 20 minutes, control the temperature at 15°C, add dropwise a mixture of 50g of berberine and doxorubicin under agitation, complete the dropwise addition in 0.5 hours, sonicate for 5 minutes, add 6g of bovine serum albumin, and heat up to 30 ℃, stirred for 3 hours, the reaction solution was dialyzed for 72 hou...

Embodiment 2

[0032] (1) Preparation of folic acid modified chitosan: Add 5g of folic acid to 2000g of water, stir, add 3g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 2.5g of N-hydroxysuccinate Imide, stirred for 1.5 hours, added 20g chitosan, heated to 60°C, stirred for 36 hours, the mixed solution was dialyzed for 80 hours with a dialysis bag with a molecular weight of 5000, and freeze-dried to obtain folic acid modified chitosan;

[0033] (2) Preparation of bovine serum albumin-folate-modified chitosan nano drug-loading system loaded with berberine and doxorubicin: take the folic acid-modified chitosan prepared in step (1) and 2g graphene oxide, and add them to 2000g water , sonicate for 40 minutes, control the temperature at 25°C, add dropwise a mixture of 150g of berberine and doxorubicin under agitation, complete the dropwise addition in 1 hour, sonicate for 10 minutes, add 12g of bovine serum albumin, and heat up to 50°C , stirred for 6 hours, the reaction solu...

Embodiment 3

[0036] ⑴ preparation of folic acid modified chitosan: 3g folic acid is added in 1500g dimethyl sulfoxide, stir, add 2.5g1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 2.0g of N-hydroxysuccinimide, stirred for 1 hour, added 15g of chitosan, heated to 50°C, stirred for 24 hours, the mixed solution was dialyzed for 76 hours with a dialysis bag with a molecular weight of 5000, and freeze-dried to obtain folic acid modified chitosan ;

[0037] ⑵Preparation of bovine serum albumin-folate-modified chitosan nano drug-loading system loaded with berberine and doxorubicin: take the folic acid-modified chitosan prepared in step 1 and 1.4g graphene oxide, add to 1500g In water, sonicate for 30 minutes, control the temperature at 20°C, add dropwise a mixture of 90g of berberine and doxorubicin under agitation, complete the dropwise addition in 0.8 hours, sonicate for 8 minutes, add 9g of bovine serum albumin, and heat up to 40 ℃, stirred for 5 hours, the reaction solution ...

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Abstract

The invention relates to the technical field of polysaccharides, in particular to a bovine serum albumin-folic acid modified chitosan nano drug delivery system loaded with berberine and doxorubicin and a preparation method of the bovine serum albumin-folic acid modified chitosan nano drug delivery system. According to the present invention, the two drugs such as berberine and doxorubicin are wrapped in the same nano-drug delivery system, the obtained nano-drug delivery system has the anti-inflammatory effect of berberine and the anti-tumor effect of doxorubicin, and under the combined action of the two drugs, the tumor cells can be effectively killed, the inflammatory environment around the tumor cells can be improved, and metastasis and recurrence of tumors are prevented. The nano drug delivery system prepared by the invention has a relatively good inhibition rate on tumor cells.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to a bovine serum albumin-folic acid modified chitosan nano drug loading system loaded with berberine and doxorubicin and a preparation method thereof. Background technique [0002] Doxorubicin hydrochloride is an antineoplastic drug that has been on the market for many years. It has therapeutic effects on acute leukemia, lung cancer, liver cancer, breast cancer, lymphoma, prostate cancer, bladder cancer, etc., and is widely used. However, doxorubicin hydrochloride has relatively large toxic and side effects, including: obvious bone marrow suppression, inflammatory reactions in the digestive tract, heart failure, hair loss, etc., and some patients may also have allergies, conjunctivitis, and abnormal liver function , fever and other symptoms. Due to its high toxicity and side effects, researchers have been working on the development of efficient drug delivery systems to tar...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/42A61K47/04A61K47/22A61K31/704A61K31/4375A61P35/00B82Y5/00B82Y40/00
CPCA61K47/36A61K47/42A61K47/02A61K47/22A61K31/4375A61K31/704A61P35/00B82Y5/00B82Y40/00
Inventor 秦大伟王利振
Owner QILU UNIV OF TECH
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