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A metal-catechin composite nanomaterial and its preparation method and application

A composite nanomaterial and catechin technology, which is applied in the field of metal-catechin composite nanomaterials and the preparation thereof, can solve the problems of complicated steps, difficult control of catechin release, etc., and achieves low cost, short cycle, and improved utilization. rate effect

Active Publication Date: 2022-07-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation method of this chitosan-coated catechin is complicated, relies on toxic substances such as TPP, and the catechin in the obtained folic acid-chitosan-epigallocatechin gallate nanoparticles uncontrollable release

Method used

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  • A metal-catechin composite nanomaterial and its preparation method and application
  • A metal-catechin composite nanomaterial and its preparation method and application
  • A metal-catechin composite nanomaterial and its preparation method and application

Examples

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Effect test

Embodiment 1

[0051] A preparation method of a metal-catechin composite nanomaterial, the specific steps are as follows:

[0052] (1) dissolve 3mmol (+)-catechin in 50mL water, obtain the (+)-catechin solution of 60mM; Dissolve 1mmol ferric chloride hexahydrate in 50mL water, obtain the ferric chloride hexahydrate solution of 20mM;

[0053] (2) mixing the (+)-catechin solution in step (1) with the ferric chloride hexahydrate solution, and stirring at 25° C. for 1 h to produce a dark brown precipitate;

[0054] (3) Washing the precipitate in step (2) with deionized water and alcohol, centrifuging and freeze-drying to obtain spherical nanoparticles, namely metal-catechin composite nanomaterials.

[0055] The morphology and characteristics of the metal-catechin composite nanomaterials prepared in this example were observed by scanning electron microscopy, and the scanning electron microscopy photos were as follows: figure 1 shown. Depend on figure 1 It can be seen that the morphology of the...

Embodiment 2

[0061] A preparation method of a metal-catechin composite nanomaterial, the specific steps are as follows:

[0062] (1) dissolve 3mmol (+)-catechin in 50mL water, obtain the (+)-catechin solution of 60mM; Dissolve 1mmol ferric chloride hexahydrate in 50mL water, obtain the ferric nitrate solution of 20mM;

[0063] (2) mixing the (+)-catechin solution in step (1) with the ferric nitrate solution, and stirring at 25° C. for 2 h to produce a dark brown precipitate;

[0064] (3) washing the precipitate in step (2) with deionized water and alcohol, centrifuging and freeze-drying to obtain spherical nanoparticles, i.e. metal-catechin composite nanomaterials;

[0065] The metal-catechin composite nanomaterial prepared above is applied to inhibit the adipogenic differentiation of human adipose-derived mesenchymal stem cells, and the steps are as follows:

[0066] (1) Weigh 10 mg of metal-catechin composite nanomaterials, disperse in 1 mL of phosphate buffered saline (PBS) after steri...

Embodiment 3

[0070] A preparation method of a metal-catechin composite nanomaterial, the specific steps are as follows:

[0071] (1) 1.5mmol (+)-catechin was dissolved in 50mL water to obtain 30mM (+)-catechin solution; 0.2mmol ferric nitrate was dissolved in 20mL water to obtain 10mM ferric nitrate solution;

[0072] (2) mixing the (+)-catechin solution in step (1) with the ferric nitrate solution, and stirring at 25°C for 3h to produce a dark brown precipitate;

[0073] (3) Washing the precipitate in step (2) with deionized water and alcohol, centrifuging and freeze-drying to obtain spherical nanoparticles, namely metal-catechin composite nanomaterials.

[0074] The metal-catechin composite nanomaterials prepared above are applied to inhibit the M1 polarization of THP-1 cells, and the steps are as follows:

[0075] (1) Weigh 50mg of metal-catechin composite nanomaterials, disperse in 10mL of phosphate buffered saline (PBS) after sterilization, to obtain metal-catechin composite nanomate...

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Abstract

The invention relates to a metal-catechin composite nanomaterial and its preparation method and application. The metal-catechin composite nanomaterial is a spherical nanoparticle formed by the coordination combination of metal ions and catechin, and has a diameter of 5-500 nm. It is prepared by mixing and stirring a metal salt solution and a catechin solution at a temperature of 20-80° C. through the principle of metal-organic coordination. The stability of the metal-catechin composite nanomaterial prepared by the present invention has been significantly improved, and at the same time, it is easily endocytized by cells with uptake capabilities such as stem cells, immune cells, and tumor cells, and can efficiently release catechin molecules to participate in intracellular processes , also has good degradability. At the same time, it has high efficiency in promoting osteogenesis and inhibiting adipogenesis, and can be used for bone defect repair based on stem cell-based bone tissue engineering. It can also be used as an anti-adipogenic agent to inhibit the inflammatory response of macrophages, and can be used for anti-inflammatory diseases. It can also be used for bone tissue repair assisted by macrophages.

Description

technical field [0001] The invention relates to a metal-catechin composite nano material and its preparation method and application, belonging to the technical field of biomaterials. Background technique [0002] Tea polyphenols are the first botanical drug approved by the US Food and Drug Administration (FDA). They are safe in application and have excellent biocompatibility and degradability. Catechin is a kind of green tea polyphenols, mainly containing four components: epigallocatechin (EGC), epicatechin (EC), epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). Catechin can promote osteogenic differentiation of mesenchymal stem cells, inhibit adipogenesis, and exhibit anti-obesity effects in animals and humans; it also has various physiological functions such as anti-oxidation, anti-inflammation, inhibition of bacteria and viruses, and anti-tumor , has broad biomedical application prospects. In addition, catechins are low cost, biodegradable, and have good b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62A61K31/555A61P19/00A61P29/00C12N5/0775C12Q1/02
CPCC07D311/62A61K31/555A61P19/00A61P29/00C12N5/0667G01N33/6863C12N2500/30
Inventor 王书华孔颖刘锋桑元华李刚薛皓刘宏
Owner SHANDONG UNIV
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