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Three-layer bionic skin scaffold based on 3D printing technology and preparation method thereof

A 3D printing and 3D printer technology, which is applied in medical science, prosthesis, additive processing, etc., can solve the problem of non-subcutaneous tissue simulation, achieve low immunogenicity, good mechanical properties, and avoid secondary damage

Inactive Publication Date: 2021-07-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, patent CN201610793440.6 and patent CN201610499353.X respectively reported a tissue-engineered skin constructed of sodium alginate hydrogel scaffold and a micro-nano composite double-layer skin scaffold, but these two only simulated the epidermis of normal skin and dermis, the subcutaneous tissue below the skin epidermis and dermis is not simulated

Method used

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  • Three-layer bionic skin scaffold based on 3D printing technology and preparation method thereof
  • Three-layer bionic skin scaffold based on 3D printing technology and preparation method thereof
  • Three-layer bionic skin scaffold based on 3D printing technology and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] 1. Preparation of hybrid bioink

[0073] Get freeze-dried GelMA (52% grafting rate) and dissolve in the PBS solution of 45 ℃, then add photoinitiator LAP and photoresist phenol red (MV absorber), make by 8%GelMA, 0.3%LAP Mixed bio-ink with 0.05% photoresist, keep the EP tube containing the ink in a 45°C water bath and wait for printing, and the above operation is protected from light.

[0074] 2. 3D printed three-layer bionic skin scaffold

[0075](1) 3D printing

[0076] The three-dimensional model of the above-mentioned three-layer bionic scaffold is used as a model (the epidermis of the skin scaffold model is a dense structure with a height of 400 μm; the dermis is a six-hole structure with a diameter of 200 μm and a height of 1200 μm; the subcutaneous tissue layer is a structure with six holes with a diameter of 400 μm , height 1000 μm; overall length 10600 μm, width 10600 μm, height 2600 μm), import the pre-designed skin scaffold STL file into the DLP photocuring...

Embodiment 2

[0080] 1. Preparation of hybrid bioink

[0081] Get freeze-dried GelMA (52% grafting rate) and HAMA (33% grafting rate) and dissolve them in the PBS solution at 45°C, then add photoinitiator LAP and photoresist phenol red (UV absorber), A mixed bio-ink composed of 8wt% GelMA, 1wt% HAMA, 0.3wt% LAP and 0.05wt% photoresist was prepared, and the EP tube containing the ink was kept in a 45°C water bath for printing, and the above operations were protected from light.

[0082] 2. 3D printed three-layer bionic skin scaffold

[0083] (1) 3D printing

[0084] The three-dimensional model of the above-mentioned three-layer bionic scaffold is used as a model (the epidermis of the skin scaffold model is a dense structure with a height of 400 μm; the dermis is a six-hole structure with a diameter of 200 μm and a height of 1200 μm; the subcutaneous tissue layer is a structure with six holes with a diameter of 400 μm , height 1000 μm; overall length 10600 μm, width 10600 μm, height 2600 μm...

Embodiment 3

[0088] 1. Preparation of hybrid bioink

[0089] Get freeze-dried GelMA (52% grafting rate) and HAMA (33% grafting rate) and dissolve them in the PBS solution at 45°C, then add photoinitiator LAP and photoresist phenol red (UV absorber), A mixed bio-ink composed of 8wt% GelMA, 1.5wt% HAMA, 0.3wt% LAP and 0.05wt% photoresist was prepared, and the EP tube containing the ink was kept in a 45°C water bath for printing, and the above operations were protected from light .

[0090] 2. 3D printed three-layer bionic skin scaffold

[0091] (1) 3D printing

[0092] The three-dimensional model of the above-mentioned three-layer bionic scaffold is used as a model (the epidermis of the skin scaffold model is a dense structure with a height of 400 μm; the dermis is a six-hole structure with a diameter of 200 μm and a height of 1200 μm; the subcutaneous tissue layer is a structure with six holes with a diameter of 400 μm , height 1000 μm; overall length 10600 μm, width 10600 μm, height 260...

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Abstract

The invention discloses a three-layer bionic skin scaffold based on a 3D printing technology and a preparation method of the three-layer bionic skin scaffold, and belongs to the technical field of tissue engineering. The skin stent is divided into three layers, wherein the first layer is a compact layer simulating epidermis; the second layer and the third layer are loose layers simulating dermis and subcutaneous tissues respectively, and are provided with mutually communicated micro-channels and different pore diameters; based on a 3D printing method, the three-layer bionic skin scaffold is prepared through specifically arranged bio-ink, the whole scaffold has the advantages of being high in swelling ratio, controllable in degradation, free of cytotoxicity, antibacterial, low in immunogenicity, capable of promoting high-quality repair of deep wounds and the like, and the skin scaffold and degradation products of the skin scaffold have good biocompatibility.

Description

technical field [0001] The invention relates to a three-layer bionic skin scaffold based on 3D printing technology and a preparation method thereof, belonging to the technical field of tissue engineering. Background technique [0002] As the largest organ of the human body, the skin is divided into the epidermis, dermis and subcutaneous tissue from the outside to the inside, covering the whole body. One of the most vulnerable organs of the human body. Many types of injury, such as contusions, cuts, chemical and thermal burns, can disrupt the integrity of the skin and cause loss of some amount of normal tissue. For some minor skin injuries, the skin can quickly repair and regenerate itself; but for deep and large open wounds (involving loss of dermis and subcutaneous tissue) caused by severe burns, diabetic foot ulcers, etc., only through the body's self It is very difficult to repair. Even if the wound is healed successfully, it will still be accompanied by a large area of...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/38A61L27/58A61L27/50A61L27/60B33Y70/00
CPCA61L27/20A61L27/222A61L27/3834A61L27/50A61L27/58A61L27/60B33Y70/00C08L89/00C08L5/08
Inventor 邢健周娟陈皓陈敬华
Owner JIANGNAN UNIV
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