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Application of rhizoma polygonati in preparation of medicine for preventing and treating low-dose or/and chronic uranium exposure

A low-dose, drug-based technology, applied in drug combinations, anti-toxins, and pharmaceutical formulations, can solve problems such as increased bleeding risk, low psychological acceptance, and gastrointestinal side effects, and achieve improved mitochondrial function, scientific design, and improved The effect of cell viability

Active Publication Date: 2021-07-13
MATERIAL INST OF CHINA ACADEMY OF ENG PHYSICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ZnSO 4 , NaHS is used as an antioxidant derived from chemical drugs, and there are problems such as timely administration, low psychological acceptance, long-term administration toxicity, etc.
Naturally derived antioxidant melatonin has side effects such as dizziness, fatigue, and worsened breathing difficulties; fish oil has a fishy odor and gastrointestinal side effects; Ginkgo biloba extract can increase the risk of bleeding

Method used

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  • Application of rhizoma polygonati in preparation of medicine for preventing and treating low-dose or/and chronic uranium exposure
  • Application of rhizoma polygonati in preparation of medicine for preventing and treating low-dose or/and chronic uranium exposure
  • Application of rhizoma polygonati in preparation of medicine for preventing and treating low-dose or/and chronic uranium exposure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment discloses the preparation method of polygonatum polysaccharide, specifically:

[0038] Take Rhizoma Polygonatum, crush it into fine powder, take about 15g, weigh it accurately, add 150mL of 95% ethanol to reflux for extraction for 1h, filter, add 120mL of distilled water to the residue, reflux for 1h, filter, concentrate the filtrate to 45mL at 60°C, add absolute ethanol , to 80% ethanol concentration, stand at 4°C for 24 hours, filter, add appropriate amount of activated carbon and sevage reagent to the filter residue for purification, and freeze-dry to obtain Polygonatum polysaccharide. Polygonatum polysaccharides were dissolved in ultrapure water to prepare a 0.5 mg / mL polysaccharide solution, filtered through a 0.22 μm filter membrane, and stored at 4°C. Dilute with DMEM / F12 medium (containing 3% fetal bovine serum and 1% third antibody) to the concentration required for the experiment.

Embodiment 2

[0040] This embodiment discloses the preparation method of Rhizoma Polygonati water extract, specifically:

[0041] Take about 100g of Polygonatum decoction pieces, weigh them accurately, add 10 times the amount of distilled water to soak for 1 hour, heat, and continue heating for 1 hour after slightly boiling, filter, add 8 times the amount of distilled water to the residue and continue heating for 0.5 hours, combine the filtrates, and concentrate under reduced pressure to 200mL. That is, the concentration of the drug solution is 0.5 g / mL. Filter through a 0.22 μm membrane filter and store at 4°C. Dilute with DMEM / F12 medium (containing 3% fetal bovine serum and 1% third antibody) to the concentration required for the experiment.

experiment example 1

[0043] This experimental example discloses the experiment on the influence of Polygonatum polysaccharide and Polygonatum water extract on the viability of HK-2 cells.

[0044] 1. Materials and methods

[0045] 1.1 Polygonatum Polysaccharides: Polygonatum polysaccharides were prepared by the method of Example 1 using the medicinal material of Radix Polygonatum as raw materials.

[0046] 1.2 5-steamed Polygonatum polysaccharides: using five-steamed and five-processed Polygonatum decoction pieces as raw materials, the 5-steamed Polygonatum polysaccharides were prepared according to the method in Example 1.

[0047] 1.3 Polygonatum water extract: using Polygonatum medicinal material as raw material, the water extract of Polygonatum was prepared according to the method in Example 2.

[0048] 1.4 5-steamed Polygonatum water extract: using five-steamed and five-processed Polygonatum decoction pieces as raw materials, the 5-steamed Polygonatum water extract was prepared according to ...

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Abstract

The invention discloses application of rhizoma polygonati in preparation of a medicine for preventing and treating low-dose or / and chronic uranium exposure, belongs to the technical field of medicines, and solves the technical problems that no low-toxicity and high-efficiency uranium detoxification medicine is available for being administered preventively and taken for a long time. The invention discloses application of rhizoma polygonati in preparation of the medicine for preventing and treating low-dose uranium exposure or / and chronic uranium exposure. The medicine has an inhibiting effect on a mitochondrial apoptosis pathway and has an activating effect on an endogenous antioxidant pathway. The medicine has good effects of preventing and treating low-dose uranium exposure and chronic uranium exposure. Experiments show that the polygonatum polysaccharide and the water extract can significantly improve the activity of HK-2 cells, reduce the apoptosis of the HK-2 cells and improve the mitochondrial function, and the polygonatum polysaccharide and the water extract inhibit the uranium-induced cytotoxic effect by inhibiting a mitochondrial apoptosis pathway and activating an endogenous antioxidant GSK-3beta / Fynin / Nrf2 pathway.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of Polygonatum in the preparation of medicines for the prevention and treatment of low-dose or / and chronic uranium exposure. Background technique [0002] As an important actinide element, uranium has dual effects of radioactivity and chemical toxicity. Owing to the extensive mining of uranium and its use in military and civilian fields, the health risks of occupational workers and the general public from long-term exposure to low levels of uranium are significantly increased. According to a large number of epidemiological, clinical and laboratory studies, uranium can enter the human body through inhalation, ingestion or skin contact, resulting in long-term deposition in the kidneys, liver and bones. Deposited uranium can trigger severe toxic effects through oxidative stress, metabolic disturbances, genetic damage and inflammation, leading to cellul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969A61K31/715A61P39/02A61P39/06C08B37/00A61K125/00
CPCA61K36/8969A61K31/715A61P39/02A61P39/06C08B37/0003
Inventor 唐伟马家骅李文靖陈春于立冰褚健李希建熊忠华
Owner MATERIAL INST OF CHINA ACADEMY OF ENG PHYSICS
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