Hydrazone amide derivative and pharmaceutical composition
A compound and hydrate technology, applied in the field of biomedicine, can solve the problems of genetic and reproductive toxicity, and achieve the effect of simple structure, good safety and low toxicity
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Embodiment 1
[0086] Synthesis of embodiment 1 hydrazone amide derivatives
[0087]
[0088] The synthetic routes of hydrazone amide derivatives are shown above. Substituted acetoacetic acid, dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP) and substituted alcohol or ammonia were added to dichloromethane, and stirred at room temperature for 8 h. The solvent was removed using a rotary evaporator, and purified by column chromatography to obtain the corresponding acetoacetamide or acetoacetate.
[0089] Add ammonia with different substitutions to methanol, add the same amount of hydrochloric acid and sodium nitrite, stir at room temperature for 0.5 hours, then add substituted acetoacetamide or acetoacetate, stir at room temperature for 10 hours, and filter to obtain the desired hydrazone Amide derivatives.
Embodiment 2
[0090] The synthesis of embodiment 2 compound 1
[0091] According to the method described in Example 1, 186 mg of ethyl 2-amino-4-methylthiazole-5-carboxylate, 206 mg of DCC, 10 mg of DMAP and 102 mg of 3-oxobutyric acid were reacted together to obtain 200 mg Ethyl-4-methyl-2-acetoacetylthiazolamine-5-carboxylate, yield 74%.
[0092] 135 mg of ethyl-4-methyl-2-acetoacetylthiazolamine-5-carboxylate, 0.5 ml of 1M hydrochloric acid, 34 mg of sodium nitrite and 70 mg of 2-aminobenzoic acid were reacted together to obtain 150 mg of the compound 1. The yield is 72%.
Embodiment 3
[0093] The synthesis of embodiment 3 compound 2
[0094] According to Example 1, 186 mg of ethyl 2-amino-4-methylthiazole-5-carboxylate, 206 mg of DCC, 10 mg of DMAP and 102 mg of 3-oxobutyric acid were reacted together to obtain 200 mg of Ethyl-4-methyl-2-acetoacetylthiazolamine-5-carboxylate, yield 74%.
[0095] 135 mg of ethyl-4-methyl-2-acetoacetylthiazolamine-5-carboxylate, 0.5 ml of 1M hydrochloric acid, 34 mg of sodium nitrite and 70 mg of 4-aminobenzoic acid were reacted together to obtain 160 mg of the compound 2. The yield is 77%.
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