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Method for preparing bacillus cereus and application of bacillus cereus

A technology of Bacillus cereus and cells, which is applied in the field of biological products, can solve problems such as inconsistent spore formation, unstable product quantity and quality, and low fermentation level, and achieve shortened fermentation time, high Bacillus cereus cells and Sporulation rate, suitability for industrial production

Active Publication Date: 2021-07-16
HANGZHOU GRAND BIOLOGIC PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current Bacillus fermentation process generally has defects such as low fermentation level, inconsistent spore formation, and unstable product quantity and quality. It is still necessary to further improve the growth and stability of Bacillus cereus.

Method used

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  • Method for preparing bacillus cereus and application of bacillus cereus
  • Method for preparing bacillus cereus and application of bacillus cereus
  • Method for preparing bacillus cereus and application of bacillus cereus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The optimization of embodiment 1 culture condition

[0030] Preparation of reagents

[0031] BA liquid medium: Prepared according to the ratio of 1% peptone, 0.5% sodium chloride, and 0.5% beef extract, adjusted the pH of the medium to 7.4 with 10% sodium hydroxide, and sterilized at 121°C for 30 minutes.

[0032] BA plate: add 2% agar powder on the basis of BA liquid medium, adjust the pH to 7.4, and sterilize at 121°C for 30 minutes. When the temperature of the medium drops to 45-50°C, pour the plate and place it in a 4°C refrigerator for later use.

[0033] 18% acetic acid solution: mix an equal volume of 36% acetic acid solution with purified water, sterilize, and set aside.

[0034] 10% sodium hydroxide solution: prepare sodium hydroxide solution according to the ratio of 10% (w / v), sterilize for 30 minutes, and set aside.

[0035] Freeze-drying protectant: prepare a freeze-drying protectant according to the ratio of 20% skimmed milk powder and 0.5% lactose, stir...

Embodiment 2

[0097] The present embodiment provides a kind of preparation method that contains Bacillus cereus bacterial powder powder, and the steps are as follows:

[0098] 1) Raw material pretreatment: According to the weight percentage of the product, Bacillus cereus bacterial powder obtained under different ventilation conditions, Bifidobacterium infantis bacterial powder produced in the workshop, Lactobacillus acidophilus bacterial powder, Enterococcus faecalis bacterial powder and pharmaceutical Acceptable excipients such as lubricants, fillers, sweeteners, etc. are pretreated.

[0099] 2) Preparation by mixing: pass the auxiliary materials through a sieve with a certain mesh number, add them into a batch mixer and mix them evenly, and finally add Bacillus cereus powder. Bacillus cereus bacteria powder and auxiliary materials were mixed in the mixer for 50 minutes and then discharged.

[0100] 3) Molding: Use a powder packaging machine to pack the sample mixed in step 2) into bags,...

Embodiment 3

[0102] The present embodiment provides a method for preparing capsules containing Bacillus cereus bacterial powder, the steps are as follows:

[0103] 1) Raw material pretreatment: According to the weight percentage of the product, Bacillus cereus bacterial powder obtained under different ventilation conditions, Bifidobacterium infantis bacterial powder produced in the workshop, Lactobacillus acidophilus bacterial powder, Enterococcus faecalis bacterial powder and pharmaceutical Acceptable excipients such as lubricants, fillers, sweeteners, etc. are pretreated.

[0104] 2) Preparation by mixing: pass the auxiliary materials through a sieve with a certain mesh number, add them into a batch mixer and mix them evenly, and finally add Bacillus cereus powder. Bacillus cereus bacteria powder and auxiliary materials were mixed in the mixer for 50 minutes and then discharged.

[0105] 3) Molding: use the capsule filling machine to put the sample mixed in step 2) into capsules, and ob...

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Abstract

The invention provides a method for increasing the amount of thalli obtained by fermentation of bacillus cereus, increasing the proportion of spores generated from the bacillus cereus and improving the storage stability of the bacillus cereus. The method comprises the following steps: in the fermentation culture process of bacillus cereus, air is introduced into the bottom of a fermentation tank, and the introduction speed of the air is 1-10 L / min, preferably 7-10 L / min. Therefore, by adjusting the fermentation process, the amount of thalli obtained by fermentation of the bacillus cereus, the proportion of spores generated from the bacillus cereus and the storage stability can be greatly improved.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a method for preparing Bacillus cereus and its application. Background technique [0002] Bacillus cereus is a kind of Gram-positive bacillus, which widely exists in animal intestines and natural environments such as soil and air, and can produce stress-resistant spores, so it is relatively resistant to external pressures such as high temperature, dryness, and pH. high. In Bacillus cereus, except for a few strains, most of Bacillus cereus can form a good symbiotic system with animals and plants, and produce beneficial substances to promote the growth of animals and plants. Because of this, Bacillus cereus is widely used in medicine, It has a wide range of applications in agriculture, feed and other fields. [0003] Bacillus cereus, as a commonly used beneficial bacterium, enters the intestinal tract, consumes excessive oxygen in the intestinal tract, creates an anaerobic envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A23L33/135C12R1/085
CPCC12N1/20A61K35/742A23L33/135
Inventor 付喜爱闫天文沈玉保程申王嘉雯
Owner HANGZHOU GRAND BIOLOGIC PHARMA INC
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