An Optical Fiber Biosensor and Its Application in Homogeneous Chemiluminescence Biodetection
A homogeneous chemiluminescence, biosensor technology, applied in the field of biochemical analysis, can solve the problems of low concentration, hysteresis non-specific signal, slow reaction rate, etc., to achieve the effect of simple operation, wide linear range, and reduced interference
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Embodiment 1
[0059] Example 1 Validation of time-resolved substrate concentration-controlled time-resolved sensors in fiber optic sensors
[0060] The biorecognition molecule immobilized on the fiber optic probe was used as the capture antibody for procalcitonin, and the chemiluminescence substrate was acridinium ester as an example to verify the substrate concentration-controlled homogeneous chemiluminescence fiber optic sensor.
[0061] Test 1) 10 μg / mL procalcitonin capture antibody and a sufficient amount of chemiluminescent substrate acridinium ester-BSA (50 mg / mL) were modified on the surface of the optical fiber core to form an optical fiber probe for procalcitonin detection. The fiber optic probe was then reacted with procalcitonin standard (10 ng / mL) and HRP-labeled procalcitonin detection antibody to form a sandwich structure, followed by adding a sufficient amount of trigger hydrogen peroxide to generate a chemiluminescent signal. like figure 1 To record the chemiluminescence k...
Embodiment 2
[0064] Example 2 Construction of Optical Fiber Biosensor
[0065] The main component of the silica fiber core is silica, and the biological recognition molecules are fixed to the fiber core through chemical bonds.
[0066] 1. Fabrication of fiber optic biosensors
[0067] Taking the biorecognition molecule immobilized by the optical fiber biosensor as the procalcitonin capture antibody, and the chemiluminescent substrate as acridine ester as an example, the preparation process is described. The specific process is as follows:
[0068] 1) Cut the optical fiber into 6cm pieces, and use a knife to gently scrape off the acrylic protective layer of 0.5cm length at one end of the optical fiber;
[0069] 2) use 24% hydrofluoric acid to corrode the part where the acrylic protective layer is removed for 20min to remove the silicon dioxide coating, rinse three times with 0.1mol / L NaOH and water respectively, and dry with nitrogen;
[0070] 3) Soak the core part with the coating layer ...
Embodiment 3
[0083] Embodiment 3 Detection of procalcitonin in blood
[0084] The principle of detecting procalcitonin is as follows: First, the conjugate of the chemiluminescent substrate acridinium ester and BSA and the procalcitonin capture antibody are respectively modified on the optical fiber to prepare a chemiluminescent optical fiber biosensor for detecting procalcitonin. Then, the sensor is reacted with the sample to be tested and the HRP-labeled procalcitonin detection antibody, and an immune sandwich reaction occurs between the capture antibody, the test substance, and the detection antibody on the sensor to form a complex. The concentration of the sample to be tested is related. Then a sufficient amount of triggering agent hydrogen peroxide is introduced, and the HRP bound on the sensor catalyzes the hydrogen peroxide to produce an oxidative product, which immediately reacts with the acridine ester modified on the optical fiber to generate a luminescent signal, and the intensit...
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