Stem cell culture device and culture method thereof

A technology of stem cell culture and petri dish, which is applied in the field of stem cell culture, can solve the problems of low work efficiency and increased pollution risk, and achieve the effect of simple and convenient use, beneficial to obtain nutrition and respiratory metabolism, and avoid excessive temperature emission

Inactive Publication Date: 2021-07-20
湖北明德健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The neural stem cell culture device in the above-mentioned patent has the following disadvantages: During the long-term culture process, the culture tube needs to be opened repeatedly to inject the nutrient solution in sequence, the work efficiency is low and the risk of contamination is greatly increased

Method used

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  • Stem cell culture device and culture method thereof
  • Stem cell culture device and culture method thereof
  • Stem cell culture device and culture method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] A stem cell culture device, such as Figure 1-3 with Figure 6-8 As shown, it includes a body 1, the top outer wall of the body 1 is fixed with a liquid agent tank 6 by bolts, the top outer wall of the liquid agent tank 6 is fixed with an infusion tube 5 by bolts, and the peripheral outer wall of the infusion tube 5 is fixed with a one-way wall by bolts. The valve, the other end of the infusion tube 5 runs through the top outer wall of the body 1; the top outer wall of the body 1 is connected with the adjusting hand 4 through the rotating shaft, and the top inner wall of the body 1 is connected with the rotating column 8 through the rotating shaft, and the input of the rotating column 8 The end is connected with the output end of the adjustment hand 4 through the transmission shaft, and the peripheral outer wall of the rotating column 8 is fixed with three connecting seats 14 by bolts, and the bottom inner walls of the three connecting seats 14 are all fixed with motors...

Embodiment 2

[0050] A culture method of the stem cell culture device described in embodiment 1, such as figure 1 shown, including the following steps:

[0051] S1: After placing the petri dishes 38 on the placement tray 33 in sequence, turn on the ultraviolet lamp 9 to continuously sterilize the body 1 for 30-100 minutes;

[0052] S2: adjusting the culture environment in the body 1 through the heating component;

[0053] S3: Adjust the positions of the three connecting seats 14 on the rotating column 8. When the infrared lamp 35 on the connecting seat 14 directly below the feeding port 16 emits a ray and aligns with a marking circle on the perspective window 15, transfer the stem cells through the pipette. Culture body is moved in the petri dish 38;

[0054] S4: When the infrared lamp 35 on the connecting seat 14 directly under the infusion tube 5 emits rays and aligns with another marking circle on the perspective window 15, guide the nutrient solution from the infusion tube 5 to the sh...

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Abstract

The invention discloses a stem cell culture device and a culture method thereof, and relates to the technical field of stem cell culture. The problem that nutrient solution adding efficiency is low is solved. The device comprises a machine body, a liquid box is arranged on the outer wall of the top of the machine body, a liquid conveying pipe is arranged on the outer wall of the top of the liquid box, a one-way valve is arranged on the circumferential outer wall of the liquid conveying pipe, and the other end of the liquid conveying pipe penetrates through the outer wall of the top of the machine body; an adjusting hand is arranged on the outer wall of the top of the machine body, a rotating column is arranged on the inner wall of the top of the machine body, the input end of the rotating column is connected with the output end of the adjusting hand through a transmission shaft, and three connecting bases are arranged on the circumferential outer wall of the rotating column. The culture method of the device comprises the following steps: after culture dishes are sequentially placed on a placing disc, an ultraviolet lamp is turned on to continuously sterilize the organism for 30 to 100 minutes. A nutrient solution is rapidly added, the culture device does not need to be repeatedly opened, pollution hidden dangers are reduced, the culture increase rate is increased, and the working time is greatly saved.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a stem cell culture device and a culture method thereof. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. When detecting stem cells, it is usually necessary to culture the stem cells. In the process of culturing the stem cells, it is usually necessary to move the position of the incubator. However, vibrations are often caused during the movement of the stem cell incubator. When it is large, it will cause the culture medium inside the stem cell incubator to shake, and the stem cells cannot cause the cell incubator to shake during the culture process, which will cause cell death and increase the probability of cell death. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/34C12M1/22C12M1/12C12M1/04A61L2/10
CPCA61L2/10C12M23/10C12M23/22C12M23/48C12M23/52C12M29/04C12M29/06C12M41/12C12M41/22
Inventor 陈飞杨方园
Owner 湖北明德健康科技有限公司
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