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Application of metformin in preparation of medicine for preventing arsenic-induced lung cancer

A technology of metformin and lung cancer, applied in the field of application of metformin in the preparation of drugs for the prevention of arsenic-induced lung cancer

Inactive Publication Date: 2021-07-23
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report about the application of metformin in slowing down the occurrence of arsenic-induced lung cancer

Method used

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  • Application of metformin in preparation of medicine for preventing arsenic-induced lung cancer
  • Application of metformin in preparation of medicine for preventing arsenic-induced lung cancer
  • Application of metformin in preparation of medicine for preventing arsenic-induced lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Cell infection and grouping

[0027] BEAS-2B cells were induced by 0.25 μM sodium arsenite, which were recorded as arsenic-exposed BEAS-2B-As cells; 0.1 mM metformin and arsenic were added to treat the cells, and cultured in parallel with the control group BEAS-2B cells, every 48-72h Subculture was carried out, and the cells were continuously exposed to the corresponding test substances during the subculture and culture process until 30 passages.

[0028] (2) Effect of metformin on clone formation of BEAS-2B-As cells

[0029] The soft agar colony formation assay is mainly used to detect the anchorage-independent growth ability and tumorigenicity of cells. It is currently the most commonly used method for detecting transformed cells and tumor cells. Cells are seeded in soft agar, and the information transmission between cells is lost. Normal Human bronchial epithelial cells will undergo apoptosis and cannot proliferate to form clonal clusters, but malignantly transf...

Embodiment 2

[0042] The difference between embodiment 2 and embodiment 1 is that metformin is replaced by metformin hydrochloride, and its effect is equivalent to that of embodiment 1, so it will not be repeated here.

Embodiment 3

[0044] The difference between Example 3 and Example 1 is that the metformin powder is dissolved in normal saline, formulated into 50 mg / mL metformin injection, sterile filtered, and injected intraperitoneally into nude mice at a dose of 200 mg / (kg·d) , Carry out pharmaceutical experiment, effect is suitable with embodiment 1, repeats no more here.

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Abstract

The invention relates to the technical field of biological medicines, in particular to application of metformin in preparation of a medicine for preventing arsenic-induced lung cancer. Metformin derivatives or pharmaceutically acceptable salts are metformin hydrochloride. The dosage form of the medicine for preventing the arsenic-induced lung cancer is a tablet, a sugar-coated pill, a gelatin capsule, an injection, an oral liquid preparation or a disintegrable paste. The medicine for preventing the arsenic-induced lung cancer is administered by oral administration or injection. It is found that metformin can slow down the bronchial epithelial cell malignant transformation process caused by chronic arsenic exposure, and the metformin has important value in preparation of the medicine for preventing the arsenic-induced lung cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an application of metformin in the preparation of a drug for preventing arsenic-induced lung cancer. Background technique [0002] Arsenic and its compounds have been identified as one of the human carcinogens by the International Cancer Research Center (IARC) and the US Environmental Protection Agency (EPA), but the mechanism of arsenic-causing cancer is still unclear. The lung is an important target organ for arsenic-induced cancer. Epidemiological investigations have found that arsenic exposure is one of the definite risk factors for lung cancer. Chronic low-dose arsenic exposure can lead to transformation of human lung epithelial cells, and arsenic-induced cell transformation is a key step in arsenic-induced tumorigenesis. [0003] Malignant transformation of cells refers to the malignant phenotype changes induced by exogenous factors on cells cultured in vitro, includin...

Claims

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Application Information

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IPC IPC(8): A61K31/155A61P35/00A61P39/02
CPCA61K31/155A61P35/00A61P39/02
Inventor 施绮雯侯瑞侠
Owner ZHEJIANG UNIV OF TECH
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