Extraction process of shark chondroitin

Abstract

The invention relates to an extraction process of shark chondroitin, which comprises the following steps: (1) pretreatment of shark cartilage: boiling the shark cartilage in clear water for 3-4 hours, cooling the boiled shark cartilage, washing away protein and grease on the surface of the shark cartilage with clear water, drying the cleaned shark cartilage in an air-blowing dryer until the weight of the shark cartilage is constant, crushing the dried shark cartilage by using a crusher, and further crushing the crushed shark cartilage by using an ultrafine crusher to obtain shark cartilage ultrafine powder; (2) alkali extraction: weighing the shark cartilage ultrafine powder, adding a sodium hydroxide solution for soaking according to a solid-to-liquid ratio of 1: 30, and placing the soaked shark cartilage ultrafine powder in a shaking table for oscillation; and (3) centrifuging: putting the mixed solution subjected to oscillation hydrolysis into a centrifugal machine for centrifuging, and reserving supernate for later use after centrifuging. By using an enzymolysis-resin extraction method, the yield and purity of chondroitin sulfate are improved, and processing leftovers of shark fins and related products are fully utilized.

Description

technical field

[0001] The invention relates to the technical field of marine organism extraction, in particular to an extraction process of shark chondroitin. Background technique

[0002] Chondroitin sulfate (CS) is a kind of glycosaminoglycan with complex structure. Sugar and disaccharide units are connected by β-1,4-glucosidic bonds, generally containing 50 to 70 disaccharide units. Chondroitin sulfate is a white or slightly yellow powder, odorless, tasteless, strong water absorption, easily soluble in water to form a solution with high viscosity, insoluble in organic solvents such as ethanol, acetone and ether, and its salts are relatively stable to heat . Studies have shown that chondroitin sulfate has various biological activities, such as preventing vascular sclerosis, lowering blood fat, anticoagulation, anti-tumor, and protecting cornea. In terms of anti-arthritis, chondroitin sulfate also has a significant effect. It can inhibit the production of body joint dam...