Extraction process of shark chondroitin

A shark chondroitin and extraction process technology, applied in the field of shark chondroitin extraction process, can solve the problems of underutilization of shark cartilage, environmental pollution, waste of resources, etc., achieve good separation and extraction effect, reduce production costs, consumption small amount of effect

Inactive Publication Date: 2021-07-23
LINYI XINYUHUI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the processing waste of shark fin and related products, shark cartilage has not been fully utilized, an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of extraction process of shark chondroitin, described extraction process comprises the following steps:

[0027] (1) Pre-treatment of shark cartilage: boil shark cartilage in water for 3 hours, wash off the surface protein and grease with water after the boiled shark cartilage is cooled, and dry the cleaned shark cartilage in a blast dryer until Constant weight, the dried shark cartilage is first pulverized with a pulverizer, and then further pulverized in a superfine pulverizer to obtain superfine shark cartilage powder;

[0028] (2) Alkaline extraction: Weigh the superfine powder of shark cartilage, add sodium hydroxide solution to soak according to the solid-to-liquid ratio of 1:30, place it in a shaker for oscillation, set the temperature to 40°C, and the speed to 120r / min, and extract by oscillation for 5 hours ;

[0029] (3) Centrifugation: the mixed solution after shaking and hydrolysis is placed in a centrifuge for centrifugation, the parameters are set ...

Embodiment 2

[0038] A kind of extraction process of shark chondroitin, described extraction process comprises the following steps:

[0039] (1) Pre-treatment of shark cartilage: boil shark cartilage in water for 4 hours, wash off the surface protein and grease with water after the boiled shark cartilage is cooled, put the cleaned shark cartilage in a blast dryer and dry until Constant weight, the dried shark cartilage is first pulverized with a pulverizer, and then further pulverized in a superfine pulverizer to obtain superfine shark cartilage powder;

[0040] (2) Alkaline extraction: Weigh the superfine powder of shark cartilage, soak in sodium hydroxide solution according to the solid-to-liquid ratio of 1:30, set the temperature at 50°C, the speed at 120r / min, and shake for 4 hours;

[0041] (3) Centrifugation: the mixed solution after shaking and hydrolysis is placed in a centrifuge for centrifugation, the parameters are set to a speed of 5000r / min, and the centrifugation time is 20min...

Embodiment 3

[0050] A kind of extraction process of shark chondroitin, described extraction process comprises the following steps:

[0051] (1) Pre-treatment of shark cartilage: boil shark cartilage in clean water for 3.5 hours, wash off the surface protein and grease with clean water after the boiled shark cartilage is cooled, put the cleaned shark cartilage in a blower dryer to dry To constant weight, the dried shark cartilage is first pulverized with a pulverizer, and then further pulverized in a superfine pulverizer to obtain superfine shark cartilage powder;

[0052] (2) Alkaline extraction: Weigh the superfine powder of shark cartilage, add sodium hydroxide solution to soak according to the solid-to-liquid ratio of 1:30, set the temperature to 45°C, set the speed to 120r / min, and shake and extract for 4.5h;

[0053] (3) Centrifugation: the mixed solution after shaking and hydrolysis is placed in a centrifuge for centrifugation, the parameters are set to a rotating speed of 5000r / min,...

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PUM

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Abstract

The invention relates to an extraction process of shark chondroitin, which comprises the following steps: (1) pretreatment of shark cartilage: boiling the shark cartilage in clear water for 3-4 hours, cooling the boiled shark cartilage, washing away protein and grease on the surface of the shark cartilage with clear water, drying the cleaned shark cartilage in an air-blowing dryer until the weight of the shark cartilage is constant, crushing the dried shark cartilage by using a crusher, and further crushing the crushed shark cartilage by using an ultrafine crusher to obtain shark cartilage ultrafine powder; (2) alkali extraction: weighing the shark cartilage ultrafine powder, adding a sodium hydroxide solution for soaking according to a solid-to-liquid ratio of 1: 30, and placing the soaked shark cartilage ultrafine powder in a shaking table for oscillation; and (3) centrifuging: putting the mixed solution subjected to oscillation hydrolysis into a centrifugal machine for centrifuging, and reserving supernate for later use after centrifuging. By using an enzymolysis-resin extraction method, the yield and purity of chondroitin sulfate are improved, and processing leftovers of shark fins and related products are fully utilized.

Description

technical field [0001] The invention relates to the technical field of marine organism extraction, in particular to an extraction process of shark chondroitin. Background technique [0002] Chondroitin sulfate (CS) is a kind of glycosaminoglycan with complex structure. Sugar and disaccharide units are connected by β-1,4-glucosidic bonds, generally containing 50 to 70 disaccharide units. Chondroitin sulfate is a white or slightly yellow powder, odorless, tasteless, strong water absorption, easily soluble in water to form a solution with high viscosity, insoluble in organic solvents such as ethanol, acetone and ether, and its salts are relatively stable to heat . Studies have shown that chondroitin sulfate has various biological activities, such as preventing vascular sclerosis, lowering blood fat, anticoagulation, anti-tumor, and protecting cornea. In terms of anti-arthritis, chondroitin sulfate also has a significant effect. It can inhibit the production of body joint dam...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069C08B37/0003
Inventor 李宗宝李嘉欣
Owner LINYI XINYUHUI BIOLOGICAL TECH CO LTD
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