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Method for improving freeze-drying survival rate and storage stability of bifidobacterium

A technology of Bifidobacterium and Bifidobacterium breve, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of poor resistance to stress and reduce activity, so as to improve quality and improve self-stress resistance. , The effect of improving freeze-drying survival rate and storage stability

Active Publication Date: 2021-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to improve the freeze-dried survival rate and storage stability of bifidobacteria on the premise of saving costs, and solve the problems of reduced activity and poor stress resistance of bifidobacteria affected by environmental factors

Method used

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  • Method for improving freeze-drying survival rate and storage stability of bifidobacterium
  • Method for improving freeze-drying survival rate and storage stability of bifidobacterium
  • Method for improving freeze-drying survival rate and storage stability of bifidobacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1. Control different culture medium components to prepare Bifidobacterium longum freeze-dried powder

[0031] 1. Prepare medium:

[0032] According to the formula in Table 1, additional substances that can adjust the composition and properties of the cell membrane of Bifidobacterium longum were added to the MRS medium; all kinds of regulatory substances were dissolved in 5 mL of absolute ethanol and added to the MRS medium, and then sterilized at 115 °C. Bacteria 20min.

[0033] 2. Preparation of Bifidobacterium longum freeze-dried powder:

[0034] Use an inoculation loop to dip the Bifidobacterium longum GDMCC No.60926 bacteria liquid in the bacteria preservation tube to streak on the MRS solid medium, and culture at a constant temperature of 37°C for 36 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium , cultivated at 37°C for 12 hours to obtain a seed liquid; inoculated the seed liquid at an inoculum size of ...

Embodiment 2

[0043] Embodiment 2. Control different culture medium components to prepare Bifidobacterium bifidum freeze-dried powder

[0044] 1. Preparation of Bifidobacterium bifidum freeze-dried powder:

[0045] On the basis of Example 1, Bifidobacterium longum GDMCC No.60926 was replaced with Bifidobacterium bifidum CGMCC No.13632 to obtain Bifidobacterium bifidum freeze-dried powder 1-12.

[0046] 2. Detect the viable count of Bifidobacterium bifidum in Bifidobacterium bifidum freeze-dried powder 1-12, and calculate the freeze-dried survival rate and Bifidobacterium bifidum freeze-dried powder was stored for 2 months and compared with the freeze-dried survival rate before freeze-drying (the test results are shown in Table 3).

[0047] Result: It can be seen from Table 3 that the Bifidobacterium bifidum prepared by adding Tween 80 1g / L, β-carotene 0.5g / L and melatonin 0.5g / L in the MRS medium was prepared from Bifidobacterium bifidum. The highest freeze-dried survival rate of Bifidoba...

Embodiment 3

[0050] Embodiment 3. Control different culture medium components to prepare Bifidobacterium adolescentis lyophilized powder

[0051] 1. Preparation of Bifidobacterium adolescentis freeze-dried powder

[0052] On the basis of Example 1, Bifidobacterium longum GDMCC No.60926 was replaced with Bifidobacterium adolescentis GDMCC No.60706 to obtain Bifidobacterium adolescentis freeze-dried powders 1-12.

[0053] 2. Detect the viable count of Bifidobacterium adolescentis in Bifidobacterium adolescentis freeze-dried powder 1-12, and calculate the freeze-dried survival rate of Bifidobacterium adolescentis in Bifidobacterium adolescentis freeze-dried powder 1-12 and the The lyophilized survival rate before lyophilization was compared after the lyophilized powder was stored for 2 months (the test results are shown in Table 4).

[0054] Result: As can be seen from Table 4, the Bifidobacterium adolescentis prepared by adding Tween 80 1g / L, β-carotene 0.5g / L and melatonin 0.5g / L in the MR...

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Abstract

The invention discloses a method for improving the freeze-drying survival rate and storage stability of bifidobacterium, and belongs to the field of microbial engineering fermentation. The invention aims to solve the problems that the activity of lactobacillus fermentum is reduced and the stress resistance is poor due to the influence of environmental factors. The invention provides a method for improving the freeze-drying survival rate and storage stability of bifidobacteria. The method adopts a fermentation culture medium containing any one or more of Tween 80, carotene and melatonin for culturing the bifidobacteria. The freeze-drying survival rate of the bifidobacteria after the cell membrane of the bifidobacteria is adjusted is more than 95%, and the survival rate after the bifidobacteria is stored at 37 DEG C for 2 months is more than 65%.

Description

technical field [0001] The invention relates to a method for improving the freeze-dried survival rate and storage stability of bifidobacteria, belonging to the field of microbial fermentation engineering. Background technique [0002] Bifidobacterium (Bifidobacterium) is a kind of probiotics, it has rich probiotic functions. In recent years, in the European Union, the United States, Japan, China and other countries and regions, the types of products containing bifidobacteria have increased year by year. A large number of scientific experiments have confirmed that regular intake of bifidobacteria and its products can ensure the health of the intestinal tract. [0003] In order for bifidobacterium products to exert ideal probiotic effects, it is necessary to ensure that the number of viable bacteria in the product is sufficient, so the industry usually uses freeze-drying methods to prepare bifidobacterium probiotic powder. In order to improve the lyophilized survival rate of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12N1/04C12R1/01
CPCC12N1/20C12N1/38C12N1/04Y02A50/30
Inventor 崔树茂毛丙永钱志浩唐鑫赵建新
Owner JIANGNAN UNIV
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