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A method for extracting and separating caffeic acid from fermentation broth

A caffeic acid and fermented liquid technology, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of low caffeic acid content, weak irritation, and less impurities, and achieve low product loss and high safety , the effect of mild conditions

Active Publication Date: 2022-02-18
南京合谷生命生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Caffeic acid can be extracted from plants, but due to the lack of plant sources and the low content of extracted caffeic acid, it is difficult to form large-scale production
Caffeic acid can also be synthesized by chemical method. Although large-scale production can be achieved, and the production process is relatively mature; however, trace impurities and isomers are uncontrollable, and side reactions are prone to occur
At present, the best effect of obtaining caffeic acid is the production of biological enzymes, mainly using chlorogenic acid as the raw material, adding it to the phosphate buffer solution with a pH value of 6.81, and adjusting the pH value to 7.0 with 1mol / L sodium hydroxide , cultured with solidified C.antaretic lipase at 37°C for 24 hours, extracted the sample with ether after cultivation, dried the extracted ether solution with anhydrous sodium sulfate after cooling, and then distilled to dryness under reduced pressure to obtain caffeic acid, not only With high purity, less impurities and weak irritation, it can be directly used as a pharmaceutical raw material; however, ether is easy to produce poison and has a certain safety risk due to its low flash point

Method used

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  • A method for extracting and separating caffeic acid from fermentation broth
  • A method for extracting and separating caffeic acid from fermentation broth
  • A method for extracting and separating caffeic acid from fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting and separating caffeic acid from fermented liquid:

[0025] (1) Ceramic membrane filtration: Take 1L of fermentation broth containing caffeic acid (10.08g / L) and add it to a beaker, heat it in a water bath, raise the temperature to 90°C, and fully stir at a speed of 400rpm for 30min. Filter through a 10nm ceramic membrane, rinse with water, collect the filtrate to obtain 1.5L filtrate A;

[0026] (2) Nanofiltration membrane nanofiltration: the above-mentioned 1.5L filtrate A is subjected to nanofiltration through a nanofiltration membrane with a molecular weight cut-off of 200D, and the water solvent recovered by rotary evaporation is used for washing, and the nanofiltration pressure is controlled to 2MPa, and the permeate is collected , to obtain 2L permeate B, measure the weight of caffeic acid in the retentate and the weight of caffeic acid in permeate B;

[0027] (3) Activated carbon decolorization: Put 2L of permeated solution B above into ...

Embodiment 2

[0032] A method for extracting and separating caffeic acid from fermented liquid:

[0033] (1) Ceramic membrane filtration: Take 1L of fermentation broth containing caffeic acid (10.05g / L) and add it to a beaker, heat it in a water bath, raise the temperature to 75°C, stir fully at a speed of 200rpm for 60min, and pass through the aperture Filter through a 10nm ceramic membrane, rinse with the water solvent recovered by rotary evaporation, collect the filtrate, and obtain 1.5L filtrate A;

[0034] (2) Nanofiltration membrane nanofiltration: the above-mentioned 1.5L filtrate A is subjected to nanofiltration through a nanofiltration membrane with a molecular weight cut-off of 300D, and the water solvent recovered by rotary evaporation is used for washing, and the nanofiltration pressure is controlled to be 1.5MPa. liquid, obtain 2L permeate B, measure the weight of caffeic acid in the retentate and the weight of caffeic acid in permeate B;

[0035] (3) Activated carbon decolori...

Embodiment 3

[0040] A method for extracting and separating caffeic acid from fermented liquid:

[0041] (1) Ceramic membrane filtration: Take 1L of fermentation broth containing caffeic acid (10.03g / L) and add it to a beaker, heat it in a water bath, raise the temperature to 80°C, stir fully at a speed of 300rpm for 45min, and pass through the aperture Filter through a 10nm ceramic membrane, rinse with water, collect the filtrate to obtain 1.5L of filtrate A;

[0042] (2) Nanofiltration membrane nanofiltration: the above-mentioned 1.5L filtrate A is subjected to nanofiltration through a nanofiltration membrane with a molecular weight cut-off of 500D, and the water solvent recovered by rotary evaporation is used for washing, and the nanofiltration pressure is controlled to 2MPa, and the permeate is collected , to obtain 2L permeate B, measure the weight of caffeic acid in the retentate and the weight of caffeic acid in permeate B;

[0043] (3) Activated carbon decolorization: put the above...

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Abstract

The invention discloses a method for extracting and separating caffeic acid from fermented liquid. The fermented liquid containing caffeic acid is used as a raw material, and is obtained through membrane separation, active carbon decolorization, rotary evaporation concentration, low-temperature crystallization and centrifugal separation in sequence. The invention has mild conditions for extracting and separating caffeic acid from fermentation broth, low product loss, high purity, no organic solvent involved in the preparation process, low toxicity, high safety, green safety and environmental protection, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting and separating caffeic acid from fermentation broth, and belongs to the technical field of caffeic acid extraction and separation. Background technique [0002] Caffeic acid is an organic acid, also known as 3,4-dihydroxycinnamic acid, with the molecular formula C 9 h 8 o 4 , with a molecular weight of 180.15, can be used safely in cosmetics, and has a wide range of antibacterial and antiviral activities. Can absorb ultraviolet rays. Caffeic acid is also a hemostatic and leukocyte-increasing drug, which can shrink and coagulate microvessels, improve the function of coagulation factors, and increase white blood cells and platelets. [0003] Caffeic acid can be extracted from plants, but due to the lack of plant sources and the low content of caffeic acid extracted, it is difficult to form large-scale production. Caffeic acid can also be synthesized by chemical methods. Although large-scale production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C59/52
CPCC07C51/42
Inventor 孙磊王浩
Owner 南京合谷生命生物科技有限公司