Method suitable for high-throughput detection of acetamiprid residues in tea leaves
An acetamiprid, high-throughput technology, which is applied in the field of ELISA detection, can solve the problems that the detection results are easily affected by the environment, lack of high-throughput methods, and are not suitable for high-throughput detection, and achieve strong universality and universality. High performance and effectiveness in addressing environmental impacts
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[0052] The preparation method of the platinum-coated gold nanoparticles labeled IgG comprises the following steps:
[0053] St1. Preparation of gold nuclei: AuNPs of about 17 nm were prepared by sodium citrate reduction method; HAuCl 4 Stir the solution until it boils, keep boiling and quickly add sodium citrate solution, continue to heat and stir until the color of the solution no longer changes, continue to heat until the solution is clear and translucent, then stop the reaction, cool to room temperature, and store at 4°C for later use; among them, HAuCl 4 The volume ratio to the sodium citrate solution is (40-60):1; the stirring speed is 150-200rpm, the time for maintaining the boiling state is 3-6min, and the time for continuous heating is 8-12min; HAuCl 4 The concentration of the solution is 0.01-0.02% (w / v), and the concentration of the sodium citrate solution is 0.8-1.2% (w / v);
[0054] St2. Platinum-coated gold preparation: the layer-by-layer seed growth method was us...
Embodiment 1
[0058] A method suitable for high-throughput detection of acetamiprid residues in tea leaves. The method comprises: using a dilution method to treat tea samples, using acetamiprid as a target in the detection process, and using specific recognition systems of antigens and antibodies and Platinum-coated gold nanoparticles label the enzyme-like catalytic activity of IgG, and catalyze the oxidation of chromogenic substrates to produce oxidation products, thereby realizing high-throughput quantitative detection of acetamiprid residues in tea. Tea samples include white tea, green tea, yellow tea, oolong tea, and black tea. or black tea;
[0059] The method includes the following specific steps:
[0060] (1) Preparation of platinum-coated gold nanoparticles-labeled IgG
[0061](1-1) Preparation of gold nuclei: AuNPs of about 17 nm were prepared by sodium citrate reduction method. Add 100mL HAuCl to a 150mL Erlenmeyer flask 4 (0.01%w / v) solution and stirred on a magnetic stirrer a...
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