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Thyroid cancer prognosis condition judgment method

A thyroid, prognostic technology, applied in instruments, biomaterial analysis, measurement devices, etc., can solve problems such as lack of understanding, apoptosis, long genetic distance, etc., to achieve poor prognosis, high probability of dedifferentiation, and overall survival. and disease-specific survival reduction

Active Publication Date: 2021-07-27
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is a long genetic distance between ATC and PTC in the same patient, and the possibility that ATC cells are derived from a subpopulation of PTC cells through genetic and / or epigenetic mechanisms cannot be ruled out
[0004] CREB3L1 is a transcription factor in the UPR pathway, which is activated under the state of endoplasmic reticulum stress. The UPR signaling pathway plays a role in protecting cells in many cases, but persistent UPR leads to apoptosis. A large number of studies believe that UPR plays a role in tumorigenesis. play a positive or negative role in the development of

Method used

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  • Thyroid cancer prognosis condition judgment method
  • Thyroid cancer prognosis condition judgment method
  • Thyroid cancer prognosis condition judgment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 CREB3L1 is the master transcriptional regulator in ATC

[0035] The ability of single-cell RNA-sequencing to accurately identify and analytically characterize the state of individual cells provides opportunities to investigate intratumoral heterogeneity and the tumor microenvironment, which is critical for understanding tumor-associated mechanisms including cellular plasticity of tumors. Bioinformatics analysis can also construct models to continuously detect the cell fate process of different cell types and reveal the gene regulatory network of tumor development.

[0036] In this study, the inventors of the present invention analyzed the characterization of these tumor cells by single-cell transcriptome sequencing of PTC and ATC, and performed a comprehensive analysis of the differentiated and undifferentiated states of thyroid tissue to study their differentiation in single-cell resolution. Common and different features under different rates. Through single...

Embodiment 2

[0039] Example 2 Preparation of HX-CREB3L1 Monoclonal Antibody

[0040] Preparation of HX-CREB3L1 monoclonal antibody

[0041] Antibody preparation

[0042] 1) By finding the CREB3L1 sequence, designing and producing protein fragments ( image 3 A), constructed into the E. coli expression vector pET28a, induced expression, and purified the target protein after verification.

[0043] 2) The purified CREB3L1 protein was used as the immunogen to immunize BALB / c female mice, and the serum antibody titer of the mice was tested by evaluating the immune effect and cross-labeling detection. Collect mouse peritoneal feeder cells, take mouse spleen and fuse with myeloma cells, perform supernatant screening (ELISA, cross-ELISA detection) one week later, screen positive clone cell line antibody subclones and measure the concentration.

[0044] 3) The supernatant of the positive clones was tested for rechecking, the selected hybridoma cells were injected into BALB / c female mice, ascites...

Embodiment 3

[0061] Example 3 Application of HX-CREB3L1 Monoclonal Antibody in Immunohistochemistry

[0062] Colorectal adenocarcinoma was collected in the standard positive control group (according to the 2020 version of WHO colorectal tumor pathological classification) by comparing the HX-CREB3L1 monoclonal antibody with the control antibody (sc-514635: commercially available CREB3L1 antibody provided by Santa Cruz Biotechnology Company). The positive expression localization, positive rate, and antibody concentration dilution ratio between the patient's tumor sample as the positive control group) and the negative control group (according to the 2008 version of lymphoma pathological classification collected non-Hodgkin's lymphoma patient tumor samples as the negative control group) To test the reliability and diagnostic efficiency of CREB3L1 monoclonal antibody in immunohistochemical experiments.

[0063] Compared with the control antibody, the results showed that the HX-CREB3L antibody w...

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Abstract

The invention provides a thyroid cancer prognosis condition judgment method and an antibody, particularly relates to an application of a CREB3L1 gene to preparation of a reagent for thyroid papillary cancer prognosis condition judgment, and particularly relates to thyroid papillary cancer prognosis condition judgment.

Description

technical field [0001] The invention relates to a method for judging the prognosis of thyroid cancer, in particular to a method for judging the prognosis of papillary thyroid cancer. Background technique [0002] Thyroid cancer is the most common endocrine malignancy, accounting for 3% of all human tumors. Several histological types of thyroid cancer have been described, including papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC). PTC accounts for more than 80% of all thyroid cancers and has a good clinical prognosis, with a 10-year disease-specific survival rate of over 90%. In contrast to those associated with highly differentiated features, ATCs are undifferentiated tumors that can exhibit stem cell-like properties, have a high proliferative potential, and are resistant to current therapies. Thus, ATC is one of the deadliest tumors with a median survival of only 6-8 months and accounts for nearly half of thyroid cancer-related deaths. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577G01N33/68
CPCG01N33/57407G01N33/57484G01N33/577G01N33/68G01N2333/4703
Inventor 罗晗李志辉姜勇魏涛许恒
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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