Rhizobium rosettiformans FN2, microbial inoculum thereof and application of Rhizobium rosettiformans FN2 and microbial inoculum in field of soil improvement

A rhizobia, FN2 technology, applied in applications, soil conditioning materials, bacteria, etc., can solve the problems of loss of microbial activity, inability of soil microorganisms to adapt, and change of soil microbial adaptability, etc., to improve stress resistance, increase colonization rate, The effect of good salinity resistance

Active Publication Date: 2021-07-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the saline-alkali composition will change the adaptability of soil microorganisms to the environment, inhibit the activity of soil microorganisms, and reduce the activities of nitrifying bacteria, phosphorus bacteria and phosphatases in the soil, thereby inhibiting the nitrification ability of nitrogen in the soil and making soil microorganisms more active. Unable to adapt to the environment under adversity and reduce or even lose microbial activity

Method used

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  • Rhizobium rosettiformans FN2, microbial inoculum thereof and application of Rhizobium rosettiformans FN2 and microbial inoculum in field of soil improvement
  • Rhizobium rosettiformans FN2, microbial inoculum thereof and application of Rhizobium rosettiformans FN2 and microbial inoculum in field of soil improvement
  • Rhizobium rosettiformans FN2, microbial inoculum thereof and application of Rhizobium rosettiformans FN2 and microbial inoculum in field of soil improvement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Screening and identification of saline-alkali-tolerant nitrogen-fixing bacteria

[0057] 1. Configuration of culture medium

[0058] LB liquid medium: tryptone 5g / L, yeast extract 2.5g / L, sodium chloride 5g / L, deionized water 1000mL, pH=7.4.

[0059] LB solid medium: tryptone 5g / L, yeast extract 2.5g / L, sodium chloride 5g / L, agar 20g / L, deionized water 1000mL, pH=7.4.

[0060] Ashby nitrogen-fixing culture medium: potassium dihydrogen phosphate 0.2g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.2g, calcium carbonate 5g, mannitol 10g, calcium sulfate dihydrate 0.1g, deionized water 1000mL, pH=7- 7.5.

[0061] Ashby solid medium: potassium dihydrogen phosphate 0.2g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.2g, calcium carbonate 5g, mannitol 10g, calcium sulfate dihydrate 0.1g, agar 20g / L, deionized water 1000mL, pH=7-7.5.

[0062] 2. Isolation and screening of nitrogen-fixing bacteria

[0063] Weigh 10g of Suaeda salsa rhizosphere sa...

Embodiment 2

[0082] The saline-alkali tolerance experiment of embodiment 2 nitrogen-fixing bacteria FN2

[0083] 1. Solution configuration

[0084] Configuration of different gradient salinity solutions: set the salinity to 0.1g / L, 0.5g / L, 1.0g / L, 1.5g / L, 3.0g / L, set the alkalinity to 7.2, 7.6, 8.0, 8.4, A total of 20 treatments. Salinity is prepared with sodium chloride, and alkalinity is adjusted with hydrochloric acid and sodium hydroxide.

[0085] Configuration of non-salinity LB liquid medium: tryptone 5g / L, yeast extract 2.5g / L, deionized water 1000mL, pH natural.

[0086] 2. Determination of the growth curve of FN2 under different salinity

[0087] Inoculate the strain FN2 into the liquid LB medium and cultivate to OD=0.1 (bacteria density is about 10 8 cells / mL) and stored in a 4°C refrigerator for later use.

[0088] Inoculate the bacterium liquid of OD=1.0 with 5% inoculum amount in the culture medium of different salinities containing 10% non-salinity LB, measure the absorban...

Embodiment 3

[0090] Corn germination experiment under embodiment 3 nitrogen-fixing bacteria FN2 inoculation

[0091] Select large, round and plump corn seeds, soak them in 70% absolute ethanol for 2 minutes for disinfection, and rinse them with tap water. The salinity gradient is 0.1g / L, 0.5g / L, 1.0g / L, 1.5g / L, 3.0g / L, the alkalinity gradient is 7.2, 7.6, 8.0, 8.4, a total of 20 treatments. Cover each petri dish with filter paper, sow 10 corn seeds, inoculate 10 9 FN2 bacteria, and add the same volume of water with different salt-alkali gradients. Two parallels were set up for each saline-alkaline gradient. Azospirillum brasilense, which also has the ability to fix nitrogen, was purchased from the China General Microorganism Culture Collection Center (CGMCC) as a control. Observe how the corn seeds germinate after a week.

[0092] The results show that the germination rate of corn seeds inoculated with FN2 can reach more than 80% at any alkalinity and salinity of 3.0g / L, while the germ...

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Abstract

The invention particularly relates to a strain of Rhizobium rosettiformans FN2, a miobial inoculum thereof and application of the Rhizobium rosettiformans FN2 and microbial inoculum in the field of soil improvement. Soil salinization can seriously affect the ecological environment of soil and sustainable development of agriculture, and the microbial preparation with the nitrogen fixation effect is adopted for improving the saline-alkali soil, so that good environmental affinity is achieved, and a sustainable development mode is achieved. The invention provides a strain of Rhizobium rosettiformans FN2, and the strain has good nitrogen fixation and saline-alkaline tolerance effects, and can be used for the development of a soil improvement preparation.

Description

technical field [0001] The invention belongs to the technical field of soil improvement engineering strains, and in particular relates to a rhizobium rosette (Rhizobium rosetiformans) FN2, its bacterial agent and its application in the field of soil improvement. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The total soil area in the world is about 13.2 billion hectares, of which the usable land area is no more than 7 billion hectares, and the cultivated land area is only about 1.5 billion hectares. Among the only arable land, about 23% of the land is saline soil, covering an area of ​​up to 340 million hectares, and 37% of the soil is sodium-containing soil, c...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01B79/02A01C1/06C09K17/14C09K101/00C12R1/41
CPCC12N1/20A01B79/02A01C1/06C09K17/14C09K2101/00
Inventor 张建庄林岚周璐璐胡振谢慧君梁爽王禄山
Owner SHANDONG UNIV
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