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Universal bacillus concentrated strain production process

A bacillus and production process technology, which is applied in the field of general-purpose bacillus concentrated strain production technology, can solve the problems of the effect speed of bacillus, the spores are not easy to absorb nutrients, and the survival rate of spores is reduced, so as to improve the grinding effect and improve Long-acting, accelerated effect

Active Publication Date: 2021-07-30
SHANDONG WODIFENG BIOLOGICAL FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned related technologies, the inventor believes that the spores enter the growth stage after germination, and need to consume a large amount of nutrients during growth, and the coating effect of the carrier makes the spores isolated from the outside world, and the spores isolated from the outside world are not easy to absorb in the external soil. Nutrients, resulting in a decrease in the survival rate of spores after germination, which affects the effective speed of Bacillus

Method used

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  • Universal bacillus concentrated strain production process
  • Universal bacillus concentrated strain production process
  • Universal bacillus concentrated strain production process

Examples

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Embodiment 1

[0050] The present application discloses a strain concentration apparatus. Refer figure 1 and figure 2 The strain concentrate comprises a fermenter 1, a pulping mechanism 2, a fluid mechanism 3, and a curing mechanism 4. When working, the operator adds the liquid medium and the preserved Bacillus strains to the fermenter 1 to prepare the bacillus fermentation broth. While fermentation, the operator processes straw and waste wood into cellulose slurry through pulping mechanism 2. When the fermentation liquid and cellulose slurry are completed, the flow of flow to the curing mechanism 4 is conveyed to the cellulose pulp, and the curing mechanism 4 is cured by curing the fermentation liquid and the cellulose, and finally obtaining concentrated strain particles.

[0051] Refer figure 2 and image 3 The fermenter 1 is provided with a stirring assembly 5, and the stirring assembly 5 includes a main stirring shaft 51, a first motor 59, and a three sub-stir shaft 52, the main stirring shaf...

Embodiment 2

[0069] This embodiment is different from that in Example 1, Step light calcium carbonate powder, wood waste and straw (2) put together in the pulverizing vessel 21, and the weight of light calcium carbonate powder of straw and wood waste the total weight ratio of 1: 2.5.

Embodiment 3

[0071] The present embodiment is different from Embodiment 2 in that, after step (2) of cellulose pulp obtained, was added to the cellulose pulp in the sheath of micro algae cultured bacteria, and at night by HPS simulated sunlight, detected after sheath micro algae cell concentration of 20% of Bacillus subtilis fermentation broth to obtain a concentration of algae containing cellulose pulp; in step (3), instead of cellulosic pulp containing cellulose pulp alginate granulated.

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Abstract

The invention relates to the technical field of strain concentration, and particularly discloses a universal bacillus concentrated strain production process which comprises the following steps: (1) culturing a bacillus strain to obtain bacillus fermentation liquor; and (2) mixing the bacillus fermentation liquor, a carrier, cellulose pulp and a cellulose hydrolase preparation in a weight ratio of 1: 2.5, granulating the mixture, and drying the mixture the obtained granules after granulation to obtain concentrated strain granules, wherein the weight ratio of the cellulose pulp to the cellulose hydrolase preparation is 1:2.5. According to the process disclosed by the invention, glucose generated after cellulose in the concentrated strain particles is decomposed can provide nutrients for the spores, and a channel communicated with the outside is generated after the cellulose is decomposed, so that the effective speed of the spores is accelerated.

Description

Technical field [0001] The present application relates to the field of strain concentration technology, and more particularly, it involves a production process of condensed strains of generalized spine bacilli. Background technique [0002] Bacillus Bacillus are widely distributed in soil, water, air, and animal intestinal intestines. When in harstrophic lack of nutrition or high temperature drought, Bacillus can suspend most of the life activities and transform into spores, Wait until the environment is appropriate, re-initiating, so there is better tolerance to harsh environments. Since a part of Bacillus can produce antibacterial substances during growth, Bacillus is also widely used in agricultural production. Currently, in the production of Bacillus formulations, in order to reduce the possibility of damage to Bacillus Bacillus, in order to reduce the transportation process, the spores are generally converted into spores, and the spores are coated through the vector, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/12C12N1/20C12M1/02C12M1/00B01J2/12D21C5/00C12R1/07C12R1/01
CPCC12N11/14C12N11/12C12N9/2437C12N1/20C12M27/08C12M27/20C12M41/20C12M41/22C12M43/00B01J2/12D21C5/005
Inventor 赵崇强孔令强孙厚真
Owner SHANDONG WODIFENG BIOLOGICAL FERTILIZER