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Detoxification method of rehmannia glutinosa libosch

A technology of rehmannia huai and rehmannia glutinosa, which is applied in the detoxification field of rehmannia huai rehmannia, can solve the problems of high production cost, cumbersome cultivation methods, and long detoxification time, and achieve the effects of long processing cycle, good quality and high yield

Inactive Publication Date: 2021-08-03
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional virus-free culture of Rehmannia glutinosa mainly adopts shoot tip culture or heat treatment combined with shoot tip culture, which are also the two most commonly used methods to solve the problem of Rehmannia glutinosa, but the virus-free efficiency of pure shoot tip culture is low, only 36% (Li Mingjun etc., Journal of Henan Normal University, 2008), and heat treatment will cause damage to a certain degree to Huai Di Huang plant, and the time of only heat treatment just needs several weeks, and detoxification time is longer, and culture method is loaded down with trivial details, and production cost is high, not necessary Suitable for industrial production

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0033] A pouring yellow detoxification method, including the following steps:

[0034] Step 1, peeling of stem tips

[0035] Under the ultra-net workbench, 40x double-eyed entry dissection of the infectious virus, peel the young leaves, cut the stem tip with 2 anion groups;

[0036] Step 2, the stem tip is detoxified:

[0037] S1, stem tip current stimulation treatment:

[0038] The cut stem tip is quickly transferred into an aluminum cassette (70 × 38 mm) placed in a sterile water, cover the aluminum cassette, and soak the aluminum cassette in an electrophoretic groove of 1% electrophoretic buffer (TAE), performing weak current stimulation. Treatment, treatment conditions are: 15mA, 5 min, inoculated with stem tip after current stimulation in MS + 0.5mg · L -1 6-benzylaine (6-ba) + 0.1mg · L -1 Naphthalene acetic acid (NAA) + 0.1mg · L -1 Bibberellin (Ga3) medium, 5 stem tips per bottle, 5 repetitions;

[0039] S2, stem tip culture:

[0040] Culture conditions: Add 30g · L above ...

Embodiment 2

[0042] Similarly to the preparation steps of Example 1, the difference is only in that step 2, the processing condition is replaced by 15 mA, 5 min, and is replaced with 5 mA, 1 min.

Embodiment 3

[0044] Similarly to the preparation steps of Example 1, the difference is only in that step 2, the processing conditions are replaced by 15 mA, 5 min, and is replaced with 5 mA, 5 min.

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Abstract

The invention relates to the technical field of medicinal plant tissue culture and detoxification culture, in particular to a detoxification method of rehmannia glutinosa libosch. The method comprises the following steps: cutting a stem tip of a rehmannia glutinosa libosch aseptic seedling infected with virus, performing stimulation treatment for 1-10 minutes in a TAE electrophoretic buffer solution with the volume fraction of 1% under the current condition of 5-30 mA, then inoculating in a culture medium for cultivation, and performing detoxification detection to obtain the detoxified rehmannia glutinosa libosch. The rapid, simple, economical and effective method is provided for detoxification of the rehmannia glutinosa libosch, early technical support is provided for factory production of detoxified seedlings of the rehmannia glutinosa libosch, and certain reference is also provided for research of detoxification technologies of other plants.

Description

Technical field [0001] The present invention belongs to the technical field of medicinal plant composition culture and detoxification culture technology, and is specifically involved in a pouring dodeca. Background technique [0002] REHMANNIA GLUTINOSA LIBOSCH. It is one of my country's famous "four yellow medicine". Long-term nutritional breeding has caused serious virus infection, resulting in decline in production, and quality degradation. According to the survey, the tobacco flower leaf virus (TMV), cucumber flower leaf virus (CMV) often monodes in the Henan Jiaozuo area, causing the decrease in land yield, decline in territation, and severely affecting the planting benefits of Huangli Yellow And clinical medicines, in order to improve the yield of Huangli, improve its quality, and make the well-used long-term development, producing healthy and viral seedlings are the main ways to control and prevent these pathogens. [0003] Traditional Huangli-viral culture mainly uses ste...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/00
Inventor 苏秀红尹素改杨林林练从龙张飞董诚明
Owner HENAN UNIV OF CHINESE MEDICINE