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Dechlorination enhancing method based on magnetic nanoparticles

A magnetic nanoparticle and dechlorination technology, applied in the field of environmental microbial engineering, can solve the problems of changing the basic properties and ecological functions of the soil, high repair costs, complex equipment and technology, etc., to achieve simple repair, increase dechlorination rate, and enhance dechlorination active effect

Active Publication Date: 2021-08-03
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, the restoration of tetrachlorethylene pollution by physical or chemical means has the disadvantages of high restoration cost, complicated equipment and process, certain applicable parameters, and possible changes in the basic properties and ecological functions of the soil.

Method used

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  • Dechlorination enhancing method based on magnetic nanoparticles
  • Dechlorination enhancing method based on magnetic nanoparticles

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Embodiment 1

[0067] In this embodiment, the original anaerobic dechlorination culture bacterial liquid identical to Comparative Example 1 is magnetically functionalized, and then the magnetically functionalized bacterial liquid is subcultured by the DEM method of the present invention, as follows:

[0068] 1. Experimental method

[0069] 1. Experimental steps such as figure 1 As shown, the details are as follows:

[0070] (1) The bacterial liquid containing anaerobic dechlorinating bacteria is magnetically functionalized using magnetic nanoparticles in an anaerobic environment. The method is as follows:

[0071] 1.1) The glass vial was aerated with 99.999% high-purity nitrogen for 2 min, sealed with a butyl rubber stopper and an aluminum cap, and then autoclaved at 121° C. for 20 min to obtain a sterile nitrogen vial.

[0072] 1.2) Use a syringe to extract the original anaerobic dechlorination culture bacteria solution and nano-magnetic particle solution into the sterile nitrogen vial in...

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Abstract

The invention discloses a dechlorination enhancing method based on magnetic nanoparticles. The method specifically comprises the steps: performing magnetic functionalization on an original anaerobic dechlorinated mixed culture in an anaerobic environment; inoculating the magnetically functionalized culture into an anaerobic culture bottle containing tetrachloroethylene, and carrying out constant-temperature shake culture; regularly measuring the content of tetrachloroethylene, and separating supernatant bacterial liquid from a precipitate by using a permanent magnet when dechlorination is started; and carrying out magnetic functionalization on thalli in the supernatant bacterial liquid again in an anaerobic environment, and inoculating the thalli into a new anaerobic culture flask containing tetrachloroethylene for passage. By applying the magnetic particles and shortening the passage time, fast-growing microorganisms in a community are enriched, and the dechlorination rate of the anaerobic dechlorination mixed culture to tetrachloroethylene is greatly improved.

Description

technical field [0001] The invention belongs to the field of environmental microbial engineering, and in particular relates to a method for enhancing dechlorination based on magnetic nanoparticles. Background technique [0002] Tetrachlorethylene is an important chemical raw material, mainly used as organic solvent, dry cleaning agent, metal degreasing solvent, fire extinguishing agent, etc. Due to the large amount of use, tetrachlorethylene has become one of the most common pollutants in groundwater, causing long-term effects on the ecosystem and affecting the liver and kidney functions, nervous system, immune system and endocrine system of the human body. Therefore, it is urgent to develop practical remediation technology to solve the environmental and health problems caused by perchlorethylene pollution. [0003] For tetrachlorethylene-contaminated groundwater or soil, commonly used remediation technologies include physical technology (gas phase extraction technology, mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N13/00
CPCC12N1/20C12N1/02C12N13/00
Inventor 沈超峰陈柯蓁刘泽钒唐晴羊嘉文陈靖文
Owner ZHEJIANG UNIV
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