Ultrasonic preparation method of oat protein and pullulan polysaccharide composite fruit and vegetable preservative film
A technology of pullulan polysaccharide and oat protein, which is applied in the field of preparing Nisin oat protein/pullulan polysaccharide composite film film packaging materials, which can solve the problems of high cost, vulnerability to mechanical damage, external microbial infection, fruit spoilage, etc. problem, to achieve the effect of extending shelf life, reducing water solubility and oxygen permeability, and reducing decay rate
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Embodiment 1
[0054] Example 1: Optimization of matrix concentration in the preparation of oat protein / pullulan polysaccharide composite membrane
[0055] (1) Prepare oat protein solution and pullulan polysaccharide solution with mass concentration of (4, 6, 8, 10, 12) g / 100mL respectively (wherein the oat protein solution pH is adjusted to 8), and oat protein with the same mass concentration The solution and the pullulan solution were mixed in equal volumes.
[0056] (2) After adding glycerin plasticizer accounting for 25% by mass of the solute (oat protein and pullulan), place it in a water bath constant temperature magnetic stirrer and stir for 30 minutes, and the water bath temperature is 70 ° C to obtain uniform oats Protein / pullulan film-forming solution.
[0057] (3) Due to the difference in the concentration of the film-forming liquid, absorb different amounts of film-forming liquid and cast it on a disposable plastic plate to form a film. The amount of solute in the plate is contr...
Embodiment 2
[0059] Example 2: Optimization of oat protein and pullulan mass ratio in the preparation of oat protein / pullulan polysaccharide composite film
[0060] (1) Prepare the oat protein solution and the pullulan solution (wherein the oat protein solution pH is adjusted to 8) with a mass concentration of 4g / 100mL respectively, and mix the two solutions according to the volume ratio (4:0, 3:1, 2 :2, 1:3, 0:4) to obtain a mixture of oat protein and pullulan with a mass ratio of 4:0, 3:1, 2:2, 1:3, 0:4.
[0061] (2) After adding glycerin plasticizer accounting for 25% of the mass percentage of the solute, place it in a water bath constant temperature magnetic stirrer and stir for 30 minutes, and the water bath temperature is 70°C to obtain a uniform oat protein / pullulan film-forming liquid .
[0062] (3) Take 15mL of film-forming solution and cast it on a disposable plastic plate to form a film, let it stand for 10 minutes, and then dry it by blowing at 60°C for 4 hours, and then place...
Embodiment 3
[0064] Example 3: Optimization of glycerin addition in the preparation of oat protein / pullulan polysaccharide composite film
[0065] (1) Prepare oat protein solution and pullulan solution with a mass concentration of 4g / 100mL respectively (the pH of the oat protein solution is adjusted to 8), and mix the oat protein solution and pullulan solution in equal volumes.
[0066] (2) After adding glycerol plasticizers (10%, 15%, 20%, 25%, 30%) in the mass percentage of the solute, place in a water bath constant temperature magnetic stirrer and stir for 30 minutes, and the temperature of the water bath is 70 °C , to obtain a uniform oat protein / pullulan film-forming liquid.
[0067] (3) Take 15mL of film-forming solution and cast it on a disposable plastic plate to form a film, let it stand for 10 minutes, and then dry it by blowing at 60°C for 4 hours, and then place it in a desiccant with a relative humidity of 43% for later use (place it in the desiccant with saturated K 2 CO 3...
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