Solid fermentation culture method of paecilomyces lilacinus

A technology of Paecilomyces lilacinus and solid fermentation, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of bacterial contamination and low spore content, and achieve sufficient oxygen and bacterial growth. The effect of exuberant, improved preservation effect

Active Publication Date: 2021-08-06
HEBI HESHENG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention provides a solid fermentation culture method of Paecilomyces lilacinus, the cultured Paecilomyces lilacinus has high live bacteria content, high purity, high follow-up preservation survival rate, and very good field

Method used

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Embodiment 1

[0028] A solid fermentation culture method of Paecilomyces lilacinus, the concrete steps are as follows:

[0029] (1) Paecilomyces lilacinus test tube activation: Prepare a test tube slant PDA medium suitable for the growth of Paecilomyces lilacinus, inoculate the pure strain of Paecilomyces lilacinus ACCC 30667, and culture it at 28°C. After 7 days of cultivation, lavender appears After the powdered conidia, it is used as a first-level test tube slant strain;

[0030](2) Preparation of Paecilomyces lilacinus primary seed liquid: prepare triangular flask liquid medium, 500ml triangular flask liquid volume is 100ml; inoculate 2 pieces of 1-square centimeter primary test tube slant strains, place at a constant temperature of 28°C Stir and cultivate at 180 rev / min on a shaker, take regular samples for inspection, and cultivate for 36 hours after confirming that there is no other bacteria contamination, and use it as a first-class seed liquid; the liquid culture medium of Paecilom...

Embodiment 2

[0039] A solid fermentation culture method of Paecilomyces lilacinus, the concrete steps are as follows:

[0040] (1) Paecilomyces lilacinus test tube activation: Prepare a test tube slant PDA medium suitable for the growth of Paecilomyces lilacinus, inoculate the pure strain of Paecilomyces lilacinus ACCC 30667, culture at 26 ° C, and cultivate for 6 days to appear lavender After the powdered conidia, it is used as a first-level test tube slant strain;

[0041] (2) Preparation of Paecilomyces lilacinus primary seed liquid: prepare triangular flask liquid medium, 500ml triangular flask liquid volume is 80ml; inoculate 2 primary test tube slant strains of 1 square centimeter, and place at a constant temperature of 27°C Stir and cultivate at 150 rev / min on a shaker, take regular samples for inspection, and cultivate for 40 hours after confirming that there is no other bacteria contamination, and use it as a first-class seed liquid; the liquid culture medium of Paecilomyces lilac...

Embodiment 3

[0050] A solid fermentation culture method of Paecilomyces lilacinus, the concrete steps are as follows:

[0051] (1) Paecilomyces lilacinus test tube activation: Prepare a test tube slant PDA medium suitable for the growth of Paecilomyces lilacinus, inoculate the pure strain of Paecilomyces lilacinus ACCC 30667, and cultivate it at 29°C. After 5 days of cultivation, lavender appears After the powdered conidia, it is used as a first-level test tube slant strain;

[0052] (2) Preparation of Paecilomyces lilacinus primary seed liquid: prepare triangular flask liquid medium, 500ml triangular flask liquid volume is 60ml; inoculate 2 pieces of 1-square centimeter primary test tube slant strains, place at a constant temperature of 27°C On a shaker, 200 rev / min stirring culture, regular sampling inspection, after confirming that there is no other bacteria contamination, cultivate for 36 hours, and use it as a first-class seed liquid; the liquid culture medium of Paecilomyces lilacinu...

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Abstract

The invention discloses a solid fermentation culture method of paecilomyces lilacinus, and belongs to the technical field of bioengineering. The invention discloses a solid fermentation culture method of paecilomyces lilacinus. The solid fermentation culture method comprises the following steps: test tube activation of paecilomyces lilacinus, preparation of a primary seed solution of paecilomyces lilacinus, preparation of a secondary seed solution of paecilomyces lilacinus, and solid fermentation culture of paecilomyces lilacinus. According to the invention, millet and rice are used as main culture materials; the acidity and a nitrogen source of a fermentation culture medium are adjusted through a corn steep liquor paste acidic material; the material viscosity in the fermentation culture process is reduced by adding white carbon black and silicon dioxide superfine water retention materials; the content of live bacteria in paecilomyces lilacinus cultured in a foam box is very high; the prepared microbial inoculum is high in purity; the subsequent preservation survival rate of the microbial inoculum is high; and the field application effect in several years is very good.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and more specifically relates to a solid fermentation culture method of Paecilomyces lilacinus. Background technique [0002] Plant parasitic nematodes can parasitize more than 2,000 kinds of plants. In recent years, the disease has spread rapidly. The area of ​​vegetable root-knot nematodes in the country has exceeded 20 million mu. It occurs in various places in my country, and the damage is very serious. Root-knot nematodes will use the stylet to puncture the host root to absorb nutrients, cut off the source of life of the plant, resulting in a decline in yield and quality. At the same time, the stylet injects secretions to stimulate the proliferation, enlargement, and transitional division of parasitic cells near the feeding point. It causes deformity of the diseased part or the formation of nodules of different sizes, commonly known as root knots, which cause the root system of the cr...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14
Inventor 胡瑞营王小琳李建欣葛桂民程玉海汪丽刚
Owner HEBI HESHENG BIO TECH CO LTD
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