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Solid phase extraction/ultra-high performance liquid chromatography-fluorescence detection method for oxypurinol in water

An ultra-high-efficiency liquid phase and oxypurinol technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve good stability, strong operability, and accurate qualitative results

Active Publication Date: 2021-08-10
辽宁省生态环境监测中心
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Problems solved by technology

[0003] At present, my country has not yet established a solid-phase extraction / ultra-high performance liquid chromatography-fluorescence detection method (SPE / UHPLC-FLD) for water quality OXY. Therefore, it is necessary to develop a sensitive , a reliable method suitable for trace OXY detection is imminent, in order to meet the urgent needs of environmental monitoring of emerging organic pollutants OXY in water quality in my country, for example, to carry out source and risk assessment of OXY pollutants in environmental water bodies, monitor and evaluate OXY Risk distribution, carry out OXY ecological and environmental impact assessment, implement OXY pollution prevention and control measures, etc.

Method used

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  • Solid phase extraction/ultra-high performance liquid chromatography-fluorescence detection method for oxypurinol in water
  • Solid phase extraction/ultra-high performance liquid chromatography-fluorescence detection method for oxypurinol in water
  • Solid phase extraction/ultra-high performance liquid chromatography-fluorescence detection method for oxypurinol in water

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Embodiment Construction

[0014] 2.1 Method principle:

[0015] Oxypurinol in water is enriched by solid-phase extraction technology, eluted with methanol, and after the eluate is concentrated, it is separated and detected by an ultra-high performance liquid chromatograph with a fluorescence detector. Qualitative by retention time, quantitative by external standard method.

[0016] 2.2 Reagents and materials:

[0017] Unless otherwise specified, the reagents used in this method are analytically pure chemical reagents that meet national standards. Unless otherwise stated, the water involved in this method is distilled water free of organic matter.

[0018] 2.2.1 Methanol (CH 3 OH): pure by liquid chromatography.

[0019] 2.2.2 Oxypurinol standard solution: ρ=1000 μg / ml, commercially available certified standard solution can be purchased. The standard solution should be refrigerated below 4°C.

[0020] 2.2.3 Standard stock solution of oxypurinol: ρ=100 μg / ml, pipette 1.00 ml of oxypurinol standard ...

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Abstract

The invention relates to a solid phase extraction / ultra-high performance liquid chromatography-fluorescence detection method of oxypurinol in water, and belongs to the technical field of analysis of oxypurinol in water. The method comprising the following steps of: enriching oxypurinol in water by adopting a solid phase extraction technology; performing eluting by using methanol; after concentrating eluent, performing separating and detecting by using an ultra-high performance liquid chromatograph with a fluorescence detector; performing nature determining according to retention time; using an external standard method for quantification, so as to realize rapid, accurate and trace analysis of the oxypurinol. The method is high in analysis speed, accurate in qualitative analysis, high in sensitivity, good in stability, high in operability and easy to popularize and use.

Description

technical field [0001] The invention belongs to the technical field of analysis of water-quality oxypurinol, in particular to a solid-phase extraction / ultra-high performance liquid chromatography-fluorescence detection method of water-quality oxypurinol. Background technique [0002] Oxypurinol (OXY) is the main active substance of allopurinol, and allopurinol is included in the World Health Organization (WHO) list of essential medicines, and is widely used in the treatment of gout and hyperuricemia in many countries . 80% of allopurinol in the human body is metabolized into OXY. OXY, one of the active pharmaceutical ingredients (APIs), can enter the environment through the following routes: excretion from drug users, improper disposal of unused drugs, discharge from manufacturing processes, and municipal sewage treatment plants. Because of its apparent high biopersistence and polarity, OXY can be found in rivers, precipitation, and groundwater, yet only very low concentra...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08G01N30/74
CPCG01N30/08G01N30/74
Inventor 赵丽娟刘枢徐天赐彭跃张峥刘雅杨冬雷刘畅赵菲
Owner 辽宁省生态环境监测中心
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