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Antigen-free collagen aggregate and preparation method thereof

An aggregate and antigen-free technology, which is applied in medical science, surgery, surgical instruments, etc., can solve the problems that the fracture strength of antigen-free collagen aggregates cannot meet the application requirements, the process is complicated, time-consuming and labor-intensive, etc., and achieve optimal fracture strength , Simplify the preparation process and reduce the production cost

Inactive Publication Date: 2021-08-13
北京欣康研医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The above-mentioned preparation method of collagen has defects such as too complicated process, time-consuming and labor-consuming, high cost, etc., and the fracture strength of the prepared antigen-free collagen aggregate cannot meet certain application requirements

Method used

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  • Antigen-free collagen aggregate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A) Take fresh and traceable bovine Achilles tendon, remove the muscle, marginal cartilage, blood vessels and fascia tissue on the surface of the bovine Achilles tendon, then cut it into thin slices with a thickness of 0.2 mm, soak it in normal saline for 30 minutes at room temperature, and then use Rinse repeatedly with double distilled water;

[0046] B) Put 10 parts by weight of bovine Achilles tendon slices in a supercritical fluid processor, add 100 parts by weight of toluene and 0.1 parts by weight of polyethylene glycol p-isooctyl phenyl ether, under pressure 10.0Mpa, room temperature Treated under the condition of 1h, after the end, rinse repeatedly with double distilled water until the beef Achilles tendon has no toluene smell to obtain the defatted beef Achilles tendon;

[0047] C) Soak the defatted beef Achilles tendon prepared in step B) in 100 parts by weight of Tris-NaCl (Tris: 0.05mol / L; NaCl: 2mol / L; pH7.5) buffer solution under ultrasonic conditions, St...

Embodiment 2

[0050] A) Take the fresh and traceable sheep Achilles tendon, remove the muscle, marginal cartilage, blood vessels and fascia tissue on the surface of the sheep Achilles tendon, then cut it into thin slices with a thickness of 0.2 mm, soak it in normal saline for 30 minutes at room temperature, and then use Rinse repeatedly with double distilled water;

[0051] B) Put 10 parts by weight of sheep Achilles tendon thin slices in a supercritical fluid processor, add 100 parts by weight of toluene and 0.1 parts by weight of polyethylene glycol p-isooctyl phenyl ether, at a pressure of 10.0Mpa, room temperature After treatment for 1 hour under the condition of 1 hour, rinse repeatedly with double distilled water until the sheep Achilles tendon has no toluene smell, then the degreased sheep Achilles tendon is obtained;

[0052] C) Soak the degreased sheep Achilles tendon prepared in step B) in 100 parts by weight of Tris-NaCl (Tris: 0.05mol / L; NaCl: 2mol / L; pH7.5) buffer solution und...

Embodiment 3

[0055] A) Take a fresh and traceable dog Achilles tendon, remove the muscle, marginal cartilage, blood vessels and fascia tissue on the surface of the dog Achilles tendon, then cut it into thin slices with a thickness of 0.2 mm, soak it in normal saline for 30 minutes at room temperature, and then use Rinse repeatedly with double distilled water;

[0056] B) Put 10 parts by weight of dog Achilles tendon thin slices in a supercritical fluid processor, add 100 parts by weight of toluene and 0.1 parts by weight of polyethylene glycol p-isooctyl phenyl ether, at a pressure of 10.0Mpa, room temperature Treated for 1 hour under the condition of 1 hour, rinsed repeatedly with double distilled water until the dog Achilles tendon has no toluene smell, then the degreased dog Achilles tendon was obtained;

[0057] C) Soak the defatted dog Achilles tendon prepared in step B) in 100 parts by weight of Tris-NaCl (Tris: 0.05mol / L; NaCl: 2mol / L; pH7.5) buffer solution under ultrasonic conditi...

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Abstract

The invention discloses an antigen-free collagen aggregate and a preparation method thereof. The highly purified antigen-free collagen aggregate is prepared by processing the achilles tendon of a mammal. The preparation method of the antigen-free collagen aggregate has the characteristics of wide raw material source, low cost, simple process and the like. The antigen-free collagen aggregate not only retains the biological activity of collagen, but also has higher mechanical strength, and can be widely applied to surgical hemostatic materials, dressings and other biomedical materials.

Description

technical field [0001] The invention relates to an antigen-free collagen aggregate and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] Collagen sutures are more suitable for surgical sutures used in wound suture after thoracic surgery. Collagen sutures are made from animal tendons. The content of pure natural collagen is high, and it has the characteristics that collagen should have. It has the advantages of complete absorption, high tensile strength, good biocompatibility, and promotion of cell growth. [0003] The main raw material used to make collagen sutures is collagen. Collagen, also known as collagen, is a helical fibrous protein twisted by three peptide chains. Collagen is an important protein in animal connective tissue. In addition to 60-70% water in connective tissue, collagen accounts for about 20-30%. Because of the high content of collagen, connective tissue has a certain structure an...

Claims

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Application Information

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IPC IPC(8): A61L17/10A61L17/08A61L17/06
CPCA61L17/06A61L17/08A61L17/10A61L2300/252A61L2430/40C08L89/00
Inventor 郑美张娜
Owner 北京欣康研医药科技有限公司
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