Multifunctional traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent and preparation method thereof

A technology of chitosan quaternary ammonium salt and organic acid, which is applied in botany equipment and methods, fungicides, pharmaceutical formulations, etc., can solve problems such as difficult to produce economic benefits, many chemical reagent residues, complicated preparation process, etc., and achieve easy Effects of operation, broad-spectrum antibacterial properties, and mild synthesis conditions

Pending Publication Date: 2021-08-13
EAST CHINA JIAOTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the composites prepared by blending are often accompanied by defects such as poor structure and performance stability, and high economic costs.
The preparation process of modifying HACC by using other chemical techniques such as cross-linking is complicated, and there are many residual chemical reagents, so it is difficult to produce good economic benefits.

Method used

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  • Multifunctional traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent and preparation method thereof
  • Multifunctional traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent and preparation method thereof
  • Multifunctional traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent and preparation method thereof

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preparation example Construction

[0037] The invention provides a preparation method of a traditional Chinese medicine organic acid grafted HACC broad-spectrum antibacterial agent, which comprises the following steps: taking the traditional Chinese medicine organic acid and HACC molecules as reaction substrates, and using EDC / NHS as an initiator to synthesize the traditional Chinese medicine organic acid grafted HACC copolymer. In some embodiments, the graft copolymer is further purified by dialysis, centrifugation, washing, freeze-drying. A pure traditional Chinese medicine organic acid grafted HACC broad-spectrum antibacterial agent is obtained.

[0038]Optional, the specific steps are as follows:

[0039] Dissolving the organic acid of traditional Chinese medicine in absolute ethanol, fully stirring until completely dissolved, and obtaining the organic acid solution of traditional Chinese medicine. The added mass of traditional Chinese medicine organic acid can be 1-4g. The traditional Chinese medicine o...

Embodiment 1

[0053] The preparation method of protocatechuic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent I (PA-g-HACC I), comprises the following steps:

[0054] (1) Dissolve 1 g of protocatechuic acid (PA) in 1 ml of absolute ethanol, stir well until completely dissolved, and obtain a protocatechuic acid ethanol solution;

[0055] (2) Dissolve 1 g of HACC in 10 ml of Tris-HCl buffer (pH=5);

[0056] (3) After mixing the above two solutions evenly, add EDC and NHS aqueous solution dropwise, so that the concentrations in the reaction system are 3mg / ml and 0.75mg / ml respectively, and mix evenly;

[0057] (4) Transfer the above mixed solution to a constant temperature shaker, react at room temperature for 12 hours, and the speed of the constant temperature shaker is 120r / min;

[0058] (5) dialyze the reaction solution obtained in step (4), dialyze with deionized water for 3 days at room temperature, and use a dialysis bag with a molecular weight cut-off ...

Embodiment 2

[0061] The preparation method of protocatechuic acid grafted chitosan quaternary ammonium broad-spectrum antibacterial agent II (PA-g-HACC II) comprises the following steps:

[0062] (1) Dissolve 3g of PA in 1ml of absolute ethanol, stir well until completely dissolved, and obtain ethanol solution of protocatechuic acid;

[0063] (2) Dissolve 1 g of HACC in 10 ml of Tris-HCl buffer (pH=5);

[0064] (3) After mixing the above two solutions evenly, add EDC and NHS aqueous solution dropwise, so that the concentrations in the reaction system are 3mg / ml and 0.75mg / ml respectively, and mix evenly;

[0065] (4) Transfer the above mixed solution to a constant temperature shaker, react at room temperature for 12 hours, and the speed of the constant temperature shaker is 120r / min;

[0066] (5) dialyze the reaction solution obtained in step (4), dialyze with deionized water for 3 days at room temperature, and use a dialysis bag with a molecular weight cut-off of 3000;

[0067] (6) Free...

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Abstract

The invention discloses a multifunctional traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent and a preparation method thereof. Under the action of EDC/NHS, a traditional Chinese medicine organic acid reacts with chitosan quaternary ammonium salt molecules to synthesize the traditional Chinese medicine organic acid-chitosan quaternary ammonium salt broad-spectrum antibacterial agent with antibacterial, anti-inflammatory and antioxidant properties. The method is simple and convenient in steps, low in investment, high in output and suitable for large-scale production and use. Through the synergistic effect of the traditional Chinese medicine organic acid and the chitosan quaternary ammonium salt, the broad-spectrum antibacterial property superior to that of the chitosan quaternary ammonium salt is obtained; through parameter optimization, the obtained traditional Chinese medicine organic acid-chitosan quaternary ammonium salt antibacterial agent has no cytotoxicity; on the other hand, the traditional Chinese medicine organic acid endows the antibacterial agent with good anti-inflammatory performance and oxidation resistance; and due to no cytotoxicity and multi-functionalization, the traditional Chinese medicine organic acid-chitosan quaternary ammonium salt broad-spectrum antibacterial agent has a wider application prospect.

Description

technical field [0001] The invention belongs to the technical field of antibacterial material preparation, and in particular relates to a traditional Chinese medicine organic acid grafted chitosan quaternary ammonium salt broad-spectrum antibacterial agent with multiple functions such as antibacterial, anti-inflammation and anti-oxidation and a preparation method thereof. Background technique [0002] There are many kinds of harmful bacteria and other microorganisms in nature, which make human beings suffer from infection and induce diseases. With the continuous improvement of social living standards and technological levels, it is very important for people to develop a broad-spectrum antibacterial agent that is safe, non-toxic, stable in performance, and good in antibacterial properties, especially for food safety and medical materials. Traditional antibacterial agents mainly include antibiotics and metal nanoparticles. These antibacterial agents have good antibacterial per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A01N43/16A01P3/00A01P1/00A61K31/785A61P31/04
CPCC08B37/003A01N43/16A61K31/785A61P31/04
Inventor 敖海勇周宸万怡灶
Owner EAST CHINA JIAOTONG UNIVERSITY
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