Pseudomonas aeruginosa 9 # and application thereof

A technology of Pseudomonas aeruginosa and rice smut bacteria, applied in the field of microorganisms, can solve the problems of affecting grain growth, affecting grain nutrient delivery, reducing rice yield and quality, etc.

Active Publication Date: 2021-08-13
CHINA NAT RICE RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the reproduction process of rice false smut bacteria, a large amount of nutrients are consumed in diseased grains, which not only harms the grain itself, but also affects the nutrient transport of adjacent grains, resulting in an increase in empty grain rate, a decrease in seed setting rate and 1,000...

Method used

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  • Pseudomonas aeruginosa 9 # and application thereof
  • Pseudomonas aeruginosa 9 # and application thereof
  • Pseudomonas aeruginosa 9 # and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Screening and Identification of Example 1 Bacterial Strain 9#

[0020] Multiple bacterial strains obtained from the soil of the China Rice Research Institute through enrichment culture, separation and purification were carried out for the antagonism experiment of rice false smut, and a bacterial strain was successfully screened through the antagonism experiment, named strain 9#. Colony morphology such as figure 1 shown. After being cultured on ordinary agar, the bacterium forms large and flat colonies, which are ground glass-like, have metallic luster and ginger smell, can produce blue-green pigments, and diffuse in the medium.

[0021] Strain 9# was identified as a strain of Pseudomonas aeruginosa by 16s sequencing and comparative analysis. The phylogenetic tree is as follows: figure 2 shown.

Embodiment 2

[0022] Embodiment 2 Antagonism test

[0023] (1) Strain culture

[0024] A, the cultivation of rice smut bacteria strain YY7850:

[0025] Inoculate the rice smut strain YY7850 preserved in our laboratory onto a potato sucrose agar medium (PSA) plate, cultivate at a constant temperature of 28°C, and inoculate it into a potato sucrose liquid medium (PS) after the colony grows, 150rpm, 28°C Cultivate at a constant temperature for 7 days, filter through eight layers of gauze to obtain a spore suspension, and set aside.

[0026] Potato sucrose agar medium (PSA): The medium adopts the PSA agar medium produced by Beijing Suo Laibao Technology Co., Ltd., and the PSA agar medium is prepared according to the amount of 47.0 g of PSA agar medium per liter of water. After packing, 121 ° C Autoclave with damp heat for 20 minutes, take out and shake well, cool and store at 4°C for later use.

[0027] Potato sucrose liquid medium (PS): Add 200g of potatoes to 1000mL of water and boil for 2...

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Abstract

The invention discloses pseudomonas aeruginosa 9 # and application thereof, and belongs to the technical field of microorganisms. The invention discloses a pseudomonas aeruginosa strain 9 #, and the preservation number of the pseudomonas aeruginosa strain 9 # is CCTCC NO: M2021178. The pseudomonas aeruginosa strain 9 # disclosed by the invention can inhibit the growth of a Ustilaginoidea virens strain YY7850, which is an example that the pseudomonas aeruginosa strain is found to have antagonism to the Ustilaginoidea virens pathogenic bacteria for the first time, and due to the fact that the strain 9 # has the capacity of inhibiting the Ustilaginoidea virens pathogenic bacteria, the strain has the potential of being developed into a Ustilaginoidea virens biocontrol bacterium.

Description

technical field [0001] The invention relates to the technical field of microbes, and more specifically relates to a strain of Pseudomonas aeruginosa 9# and its application. Background technique [0002] Rice false smut is a fungal disease on rice spikes caused by the infection of the rice false smut fungus - Ustilaginodea virens (Cooke) Takahashi. It infects glumes, glumes, and grains in the late growth stage of rice, commonly known as "harvest disease". . Rice false smut is a worldwide rice disease, which occurs in many countries such as China, Japan, the United States, India, and the Philippines. In recent years, it has been upgraded from a minor disease to one of the major diseases on rice. The smut fungus consumes a large amount of nutrients in the diseased grains during the reproduction process, which not only harms the grain itself, but also affects the nutrient transport of adjacent grains, resulting in an increase in the empty grain rate, a decrease in the seed set...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01P3/00C12R1/385
CPCC12N1/20A01N63/27
Inventor 林晓燕陈铭学曹赵云杨欢
Owner CHINA NAT RICE RES INST
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