Pseudomonas aeruginosa 9 # and application thereof
A technology of Pseudomonas aeruginosa and rice smut bacteria, applied in the field of microorganisms, can solve the problems of affecting grain growth, affecting grain nutrient delivery, reducing rice yield and quality, etc.
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Embodiment 1
[0019] Screening and Identification of Example 1 Bacterial Strain 9#
[0020] Multiple bacterial strains obtained from the soil of the China Rice Research Institute through enrichment culture, separation and purification were carried out for the antagonism experiment of rice false smut, and a bacterial strain was successfully screened through the antagonism experiment, named strain 9#. Colony morphology such as figure 1 shown. After being cultured on ordinary agar, the bacterium forms large and flat colonies, which are ground glass-like, have metallic luster and ginger smell, can produce blue-green pigments, and diffuse in the medium.
[0021] Strain 9# was identified as a strain of Pseudomonas aeruginosa by 16s sequencing and comparative analysis. The phylogenetic tree is as follows: figure 2 shown.
Embodiment 2
[0022] Embodiment 2 Antagonism test
[0023] (1) Strain culture
[0024] A, the cultivation of rice smut bacteria strain YY7850:
[0025] Inoculate the rice smut strain YY7850 preserved in our laboratory onto a potato sucrose agar medium (PSA) plate, cultivate at a constant temperature of 28°C, and inoculate it into a potato sucrose liquid medium (PS) after the colony grows, 150rpm, 28°C Cultivate at a constant temperature for 7 days, filter through eight layers of gauze to obtain a spore suspension, and set aside.
[0026] Potato sucrose agar medium (PSA): The medium adopts the PSA agar medium produced by Beijing Suo Laibao Technology Co., Ltd., and the PSA agar medium is prepared according to the amount of 47.0 g of PSA agar medium per liter of water. After packing, 121 ° C Autoclave with damp heat for 20 minutes, take out and shake well, cool and store at 4°C for later use.
[0027] Potato sucrose liquid medium (PS): Add 200g of potatoes to 1000mL of water and boil for 2...
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