Modified atmosphere keep-alive method of pearl gentian grouper
A technology for pearl gentian and grouper, which is applied in fish farming, climate change adaptation, application and other directions, can solve the problems of easy deterioration of fish body quality, easy to pierce each other, high transportation cost, and reduce the discharge of metabolic waste. , The effect of improving the carrying capacity and high survival rate
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Embodiment 1
[0019] (1) Select pearl gentian grouper with uniform size, good vitality, no damage to the body surface, and healthy and disease-free;
[0020] (2) Using freshly filtered seawater with a salinity of 20%, a water temperature of 21°C, a dissolved oxygen of 6.5 mg / L, a pH of 6.7, and a fish density of 85g / L, the pearl gentian grouper was kept without food for 24 hours;
[0021] (3) The pearl gentian grouper lowered the water temperature from 22°C to 12°C with a cooling program of 26°C, 3°C / h, 20°C, 2°C / h, 15°C, 1°C / h before controlled atmosphere preservation , kept for 1 hour, so that the fish basically lost its balance, turned over, had no obvious response to external stimuli, and the frequency of gill cover opening and closing was stable, and entered a dormant state;
[0022] (4) Wrap the dormant pearl gentian grouper with a sponge soaked in seawater at 15°C in advance, put it into an ordinary PE bag for inflatable packaging according to O2:N2=85:15, and carry out anaerobic tre...
Embodiment 2
[0025] (1) Select pearl gentian grouper with uniform size, good vitality, no damage to the body surface, and healthy and disease-free;
[0026] (2) Using freshly filtered seawater with a salinity of 21%, a water temperature of 22°C, a dissolved oxygen of 7 mg / L, a pH of 7, and a fish density of 85 g / L, the pearl gentian grouper was kept without food for 28 hours;
[0027] (3) The pearl gentian grouper lowered the water temperature from 23°C to 13°C with a cooling program of 25°C, 3°C / h, 19°C, 2°C / h, 14°C, 1°C / h before controlled atmosphere preservation , kept for 0.5 h, so that the fish basically lost its balance, turned over, had no obvious response to external stimuli, and the opening and closing frequency of the gill cover was stable, entering a dormant state;
[0028] (4) Wrap the dormant pearl gentian grouper with a sponge soaked in seawater at 15°C in advance, put it into an ordinary PE bag for inflatable packaging according to O2:N2=85:15, and carry out anaerobic treatm...
Embodiment 3
[0031] (1) Select pearl gentian grouper with uniform size, good vitality, no damage to the body surface, and healthy and disease-free;
[0032] (2) Using freshly filtered seawater with a salinity of 22%, a water temperature of 23°C, a dissolved oxygen of 7.5 mg / L, a pH of 7.3, and a fish density of 85g / L, the pearl gentian grouper was kept without food for 32 hours;
[0033] (3) Pearl gentian grouper lowered the water temperature from 22 to 23 ℃ to 12-14°C, kept for 0.6 h, the fish basically lost its balance, the body turned over, no obvious response to external stimuli, the opening and closing frequency of the gill cover was stable, and it entered a dormant state;
[0034] (4) Wrap the dormant pearl gentian grouper with a sponge soaked in seawater at 15°C in advance, put it into an ordinary PE bag for inflatable packaging according to O2:N2=85:15, and carry out anaerobic treatment at 15°C. water preservation;
[0035] (5) After keeping alive without water, move the pearl ge...
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