Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Blood free DNA protection reagent, protection method and blood collection tube

A technology for protecting reagents and blood, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of reducing DNA polymerase activity, reducing the detection sensitivity of PCR reaction, and the influence of inspection and test results.

Pending Publication Date: 2021-08-17
SHENZHEN BIOCOMMA TECH +1
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prolong the storage time of blood cell-free DNA, enzyme inhibitors are usually added to some storage reagents, but enzyme inhibitors will affect the subsequent inspection and detection of cell-free DNA
For example, in the PCR detection of free DNA, nuclease inhibitors can bind and reduce the activity of DNA polymerase, thereby reducing the detection sensitivity of the PCR reaction and affecting the inspection and detection results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood free DNA protection reagent, protection method and blood collection tube
  • Blood free DNA protection reagent, protection method and blood collection tube
  • Blood free DNA protection reagent, protection method and blood collection tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Prepare the blood free DNA protection reagent, the protection reagent contains 70mg / ml tripotassium edetate hydrate, 400mg / ml imidazolidinyl urea, 10mg / ml polyvinyl alcohol, 40mg / ml trehalose and 30mg / ml ml of glycine, and store the prepared protection reagent in a vacuum blood collection tube. The volume ratio of protective reagent to blood is 1:7. Marked as group B1.

Embodiment 2

[0053] Prepare the blood free DNA protection reagent, the protection reagent contains 70mg / ml disodium edetate dihydrate, 400mg / ml diazolidinyl urea, 10mg / ml Tween-20, 40mg / ml polyethylene Alcohol and 30mg / ml Tris-HCL, and save the prepared protective reagent in a vacuum blood collection tube. The volume ratio of protective reagent to blood is 1:7. Marked as group B2.

Embodiment 3

[0055] Prepare the blood cell-free DNA protection reagent, the protection reagent contains 70mg / ml tripotassium edetate hydrate, 400mg / ml imidazolidinyl urea, 10mg / ml Tween-20, 40mg / ml trehalose and 30mg / ml of glycine, and save the prepared protection reagent in a vacuum blood collection tube. The volume ratio of protective reagent to blood is 1:10. Marked as group B3.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a blood free DNA protection reagent, a protection method adopting the protection reagent and a blood collection tube containing the protection reagent. The protection reagent comprises 20-100 mg / ml of an anticoagulant, 100-600 mg / ml of a preservative, 10-50 mg / ml of an anti-adsorbent and 20-50 mg / ml of a buffering agent. The anticoagulant comprises at least one of tripotassium ethylenediaminetetraacetate hydrate, ethylenediaminetetraacetic acid and disodium ethylenediaminetetraacetate dihydrate, the preservative comprises at least one of imidazolidinyl urea, diazolidinyl urea and diazolidinyl urea, the anti-adsorbent comprises at least one of polyvinyl alcohol, trehalose and Tween-20, and the buffer agent comprises glycine or Tris-HCL. According to the blood free DNA protection reagent, the influence of the protection reagent on free DNA detection and detection results is reduced, and meanwhile, the blood free DNA protection reagent also has a good preservation effect on blood free DNA.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blood free DNA protection reagent, protection method and blood collection tube. Background technique [0002] Blood free DNA (cell-free DNA, cfDNA) is a degraded DNA fragment released into plasma, which exists in various body fluids of the human body, and its concentration changes with the state of the human body. It is a new type of non-invasive molecular detection technology. have important clinical value. Blood cell-free DNA has important potential value for early diagnosis of diseases. The cell-free DNA of the fetus can dissociate into the blood of pregnant women. By detecting the cell-free DNA of the fetus in the blood of pregnant women, the male sex (Y chromosome) of the fetus can be predicted, and whether it has 21-trisomy, 18-trisomy, 12-trisomy Somatic and other chromosomal diseases. Cell-free DNA also has important potential value in the prognosis and monitoring of dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 雷秋亚陈高明胡玉梅
Owner SHENZHEN BIOCOMMA TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com