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Preparation process of morchella polysaccharide and novel application thereof in prevention and treatment of thrombus and diseases caused by thrombus

A preparation process, the technology of Morchella, applied in blood diseases, cardiovascular system diseases, applications and other directions

Inactive Publication Date: 2021-08-20
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention mainly pays attention to the polysaccharides of Morchella extract. Existing studies have shown that Morchella polysaccharides have anti-inflammatory, anti-oxidation, hypoglycemic and blood lipid-lowering functions, but no research has yet shown that it has anti-thrombotic diseases.

Method used

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  • Preparation process of morchella polysaccharide and novel application thereof in prevention and treatment of thrombus and diseases caused by thrombus
  • Preparation process of morchella polysaccharide and novel application thereof in prevention and treatment of thrombus and diseases caused by thrombus
  • Preparation process of morchella polysaccharide and novel application thereof in prevention and treatment of thrombus and diseases caused by thrombus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Morchella polysaccharide has inhibitory effect on carrageenan-induced tail thrombosis in mice

[0046] 1. Extraction and purification of morel polysaccharide

[0047] Weigh 100 g of crushed morels and dissolve in 2 L of distilled water, 85 °C water bath for 2 h; filter residues dissolved in 2 L of distilled water, 85 °C water bath for 2 h; filtered filter residues re-dissolved in 1 L of distilled water, 85 °C water bath for 2 h, filter; collect the total The filtrate was centrifuged, the supernatant was taken, suction filtered, and rotary evaporated. After rotary evaporation, the solution was dissolved in absolute ethanol, stirred at 4°C for 12 hours, the supernatant was discarded, and the solution was rotary evaporated again to 100ml. After rotary evaporation, an equal volume of n-butanol: chloroform = 1:4 mixture was added to the solution, stirred, and centrifuged to remove protein precipitates. The above supernatant was rotary-evaporated to an appropriate...

Embodiment 2

[0050] Example 2 Morchella polysaccharide inhibits carrageenan-induced tail vein thrombosis in mice

[0051] 1. Mouse tail section and HE staining

[0052] Tail section: In order to determine the thrombus formed in the mouse tail vein, a piece of mouse tail was collected at different positions (distance from the tail tip), fixed in 4% paraformaldehyde universal tissue fixative for 48 hours, and then fixed in 30% paraformaldehyde The sucrose solution was dehydrated for 24h. The dehydrated tissues were embedded in OCT, sliced ​​with a cryostat, with a thickness of 5 μm, and stored in a -20°C refrigerator.

[0053] HE staining: take the frozen sections out of the refrigerator, place them at room temperature for 30 minutes, and then soak them in PBS for 5 minutes to remove embedding. The surrounding area of ​​the sample was blotted dry with absorbent paper, stained with hematoxylin solution for 1 min, rinsed with distilled water and returned to blue. Counterstain with eosin for...

Embodiment 3

[0056] Example 3 Morchella polysaccharide inhibits carrageenan-induced mouse liver and pulmonary vascular thrombosis

[0057] 1. Mouse liver and lung sections and HE staining

[0058] Liver and lung slices: In order to determine the thrombus formed in the liver and lungs of mice, cut a piece of fresh liver collected from each mouse and fix it in 4% paraformaldehyde universal tissue fixative for 48 hours, cut half The lungs were also fixed in 4% paraformaldehyde universal tissue fixative solution for 48h, and then dehydrated with 30% sucrose solution for 24h. The dehydrated tissues were embedded in OCT, sliced ​​with a cryostat, with a thickness of 5 μm, and stored in a -20°C refrigerator.

[0059] HE staining: same as Example 2.

[0060] 2. Experimental results

[0061] Such as Figure 4 and 5 As shown, we found multiple severe thrombi in the hepatic vessels of control mice. Similarly, blood clots were readily found in the lung vessels of control mice. In contrast, the ...

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Abstract

The invention discloses a preparation process of morchella polysaccharide and novel application thereof in prevention and treatment of thrombus and diseases caused by thrombus, and relates to the technical field of medicines and special medical foods. The morchella polysaccharide is obtained by extracting and purifying one or more of morchella, morchella deliciosa, morchella crassipes, double-rib morchella, morchella conica and congeneric morchella; and the diseases caused by thrombus comprise cerebral apoplexy, myocardial infarction, peripheral venous thrombosis, pulmonary embolism and the like. The prepared morchella polysaccharide can significantly inhibit carrageenan-induced mouse thrombus, mouse caudal vein thrombus formation and mouse liver and lung blood vessel thrombus formation, the morchella polysaccharide serving as an antithrombotic drug is extremely high in safety and high in medication compliance, large-scale artificial planting of morchella is achieved, and the source is wide, therefore, the morchella polysaccharide has a relatively wide application prospect in prevention and treatment of thrombus and diseases caused by thrombus.

Description

technical field [0001] The invention relates to a new application of hickory chick polysaccharide, specifically a preparation process of hickory chick polysaccharide and its new application in the prevention and treatment of thrombus and diseases caused by thrombus, and belongs to the technical field of medicine and special medical food. Background technique [0002] Thrombosis refers to blood clots caused by activation of coagulation factors and platelets during arterial and venous blood flow, and blood clots fall off to form thrombus. The formation of thrombus will hinder the normal operation of blood flow, resulting in stenosis and occlusion of the blood vessel lumen, affecting the normal operation of blood, resulting in ischemia and infarction of important organs of the body. Thrombosis includes arterial thrombosis and venous thrombosis, and common thromboembolic diseases include acute cardiovascular adverse events such as stroke, myocardial infarction, peripheral venous...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K36/062A23L33/125A61P7/02A61P9/10A61P11/00C08B37/00
CPCC08B37/0003A61K31/715A61K36/062A61P7/02A61P9/10A61P11/00A23L33/125A23V2002/00A23V2200/326A23V2250/51
Inventor 段亚君陈贵兰梁英权韩际宏王丹丹张丰杨潇潇陈元利张爽葛玉
Owner HEFEI UNIV OF TECH
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