Method for extracting hydroxytyrosol from olive fruits

A technology of hydroxytyrosol and olive fruit, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of cumbersome extraction process and low extraction efficiency of hydroxytyrosol, and prevent oxidative denaturation and reduce production costs. The effect of low cost, simple method and easy operation

Pending Publication Date: 2021-08-20
陕西富恒生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a process for efficiently extracting and preparing hydroxytyrosol from olive fruit, so as to solve the problems that the existing hydroxytyrosol extraction process is cumbersome and the extraction efficiency is low

Method used

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  • Method for extracting hydroxytyrosol from olive fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Step 1: Select clean and undamaged olive fruit raw materials for depitting, crushing, and drying to obtain olive fruit powder. Weigh 10 kg of olive fruit powder, add 30 L of sulfuric acid aqueous solution with a concentration of 0.6 mol / L for acid hydrolysis and extraction, and keep the temperature at 80 °C, while leaching, a stirrer is used to continuously stir to improve the leaching efficiency.

[0031] Step 2: The pH of the leached extract is adjusted to 5 with 1 mol / L NaOH solution to obtain a neutral extract.

[0032] Step 3: Extract the partial neutral extract with ethyl acetate for 3 times, wherein the volume ratio of the partial neutral extract to the ethyl acetate is 1:3 to obtain the hydroxytyrosol extract.

[0033] Step 4: Concentrate and crystallize the hydroxytyrosol extract, then vacuum freeze-dry at -25°C for 24 hours to obtain a finished product with a content of 45% hydroxytyrosol, and the quality of the finished product is 0.3% of the mass of the oliv...

Embodiment 2

[0035] Step 1: Select clean and undamaged olive fruit raw materials for depitting, crushing, and drying to obtain olive fruit powder. Weigh 10 kg of olive fruit powder, add 33 L of sulfuric acid aqueous solution with a concentration of 0.5 mol / L for acid hydrolysis and extraction, and keep the temperature at 83 °C, while leaching, a stirrer is used to continuously stir to improve the leaching efficiency.

[0036] Step 2: The pH of the extracted extract after leaching is adjusted to 6 with 1 mol / L NaOH solution to obtain a neutral extract.

[0037] Step 3: Extract the partial neutral extract with ethyl acetate for 3 times, wherein the volume ratio of the partial neutral extract to the ethyl acetate is 1:4 to obtain the hydroxytyrosol extract.

[0038] Step 4: Concentrate and crystallize the hydroxytyrosol extract, and then vacuum freeze-dry at -25°C for 30 hours to obtain a finished product of hydroxytyrosol with a content of 47%, and the quality of the finished product of hydr...

Embodiment 3

[0040] Step 1: Select clean and undamaged olive fruit raw materials for depitting, crushing, and drying to obtain olive fruit powder. Weigh 10 kg of olive fruit powder, add 37 L of sulfuric acid aqueous solution with a concentration of 0.4 mol / L for acid hydrolysis and extraction, and keep the temperature at 87 °C, while leaching, a stirrer is used to continuously stir to improve the leaching efficiency.

[0041] Step 2: The pH of the extracted extract after leaching is adjusted to 7 with 1 mol / L NaOH solution to obtain a neutral extract.

[0042] Step 3: extracting the neutral extract with ethyl acetate for 4 times, wherein the volume ratio of the partial neutral extract to the ethyl acetate is 1:4 to obtain a hydroxytyrosol extract.

[0043] Step 4: Concentrate and crystallize the hydroxytyrosol extract, and then vacuum freeze-dry at -25°C for 36 hours to obtain a finished product of hydroxytyrosol with a content of 48%, and the quality of the finished product of hydroxytyro...

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Abstract

The invention discloses a method for extracting hydroxytyrosol from olive fruits, which comprises the steps of weighing a proper amount of olive fruit powder, adding a sulfuric acid aqueous solution for acid hydrolysis extraction, and keeping the temperature at 80-90 DEG C; adjusting the pH value of the extracted extracting solution to 5-7 by using a NaOH solution to obtain a slightly neutral extracting solution; extracting the slightly neutral extracting solution with ethyl acetate, wherein the volume ratio of the slightly neutral extracting solution to the ethyl acetate is 1: (3-5), and a hydroxytyrosol extracting solution is obtained; and carrying out concentration and crystallization on the hydroxytyrosol extraction liquid, then conducting vacuum freeze drying, and obtaining a hydroxytyrosol finished product with the content of 45%-50%, wherein the mass of the obtained hydroxytyrosol finished product is 0.3%-0.8% of the mass of the olive fruit powder. The acid hydrolysis method is adopted for extraction, compared with an enzyme method, the production cost is low, the method is simple, convenient and easy to operate, the sulfuric acid solution is used for hydrolysis, the stability of the property of the plant extract can be kept, and oxidative denaturation of hydroxytyrosol is prevented; bacterial pollution in the extraction process is prevented; and the yield of hydroxytyrosol obtained by hydrolyzing oleuropein in the fruits can be improved.

Description

technical field [0001] The invention belongs to the technical field of hydroxytyrosol extraction, in particular to a method for extracting hydroxytyrosol from olive fruit. Background technique [0002] Hydroxytyrosol (Hydroxytyrosol, HT), chemical name: 3,4-dihydroxyphenethyl alcohol, has a strong antioxidant activity, mainly in the form of esters in the fruit and leaves of olives. HT has a simple structure and a small molecular weight, and its main pharmacological effects include anti-oxidation, antibacterial, hypoglycemic, cardiovascular protection, anti-cancer, anti-inflammatory, anti-viral, etc. Alcohol extracts are expected to become new raw materials in the fields of health care products and medicines. [0003] At present, the research on the preparation process of olive leaf extract is a hot spot in the plant extraction industry. However, since only oleuropein can be obtained from the crude extraction of olive leaves, further enzymatic hydrolysis and other processes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/82C07C37/72C07C37/84
CPCC07C37/004C07C37/82C07C37/72C07C37/84
Inventor 王德军王双宁陶正升陈小慧刘红艳
Owner 陕西富恒生物科技有限公司
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