Synthetic method of eutectic solvent and method for extracting and purifying eleutheroside B

A technology of deep eutectic solvent and synthesis method, applied in the field of medicine, can solve problems such as unfavorable purification of eleutheroside B, complex extraction process, complex composition, etc., achieve 100% utilization rate of reaction stock solution, simple processing conditions, and purification method easy effect

Pending Publication Date: 2021-08-20
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has many extracted components and complex composition, which is not conducive to the further purification of eleutheroside B, and the large use of organic solvents may cause danger to humans and the environment
[0004] In response to the above-mentioned existing technical problems, in recent years, supercritical fluid extraction technology, semi-bionic extraction technology, and e

Method used

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  • Synthetic method of eutectic solvent and method for extracting and purifying eleutheroside B
  • Synthetic method of eutectic solvent and method for extracting and purifying eleutheroside B
  • Synthetic method of eutectic solvent and method for extracting and purifying eleutheroside B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) the synthetic method of deep eutectic solvent, comprises the following steps:

[0040] (1) Mix 0.1mol choline chloride, 0.1mol L-proline, and 20mL ultrapure water, the mixing speed is 500r / min, and the mixing time is 2h;

[0041] (2) Stir the mixed components at a constant temperature. The constant temperature is 80° C., the stirring time is 2 hours, and the stirring speed is 500 r / min to synthesize a deep eutectic solvent.

[0042] (2) The method for extracting eleutheroside B, comprising the following steps:

[0043] 1) Dried Acanthopanax senticosus and ground it into powder, passed through a 60-mesh sieve, the drying temperature was 80°C, and the drying time was 2 hours;

[0044] 2) Put 1g of Acanthopanax senticosus powder in the extraction tank, add 20mL of deep eutectic solvent, and then carry out constant temperature stirring, the constant temperature is 80°C, the stirring time is 2h, and the stirring speed is 500r / min, to obtain Eleutheroside B of the extra...

Embodiment 2

[0047] (1) the synthetic method of deep eutectic solvent, comprises the following steps:

[0048] (1) Mix 0.1mol choline chloride, 0.1mol L-proline, and 10mL ultrapure water, the mixing speed is 200r / min, and the mixing time is 1.5h;

[0049] (2) Stir the mixed components at a constant temperature, the constant temperature is 85° C., the stirring time is 1.5 h, and the stirring speed is 200 r / min, to synthesize a deep eutectic solvent.

[0050] (2) The method for extracting eleutheroside B, comprising the following steps:

[0051] 1) Dried Acanthopanax senticosus and ground it into powder, passed through a 70-mesh sieve, the drying temperature was 80°C, and the drying time was 1 hour;

[0052] 2) Put 1 g of Acanthopanax senticosus powder in the extraction tank, add 30 mL of deep eutectic solvent, and then carry out constant temperature stirring, the constant temperature is 80°C, the stirring time is 2 hours, and the stirring speed is 500r / min, to obtain Eleutheroside B of the ...

Embodiment 3

[0055] (1) the synthetic method of deep eutectic solvent, comprises the following steps:

[0056] (1) Mix 0.1mol choline chloride, 0.1mol L-proline, and 30mL ultrapure water, the mixing speed is 300r / min, and the mixing time is 1.8h;

[0057] (2) Stir the mixed components at a constant temperature, the constant temperature is 90° C., the stirring time is 1.8 h, and the stirring speed is 300 r / min, to synthesize a deep eutectic solvent.

[0058] (2) The method for extracting eleutheroside B, comprising the following steps:

[0059] 1) Dried Acanthopanax senticosus and ground it into powder, passed through an 80-mesh sieve, the drying temperature was 80°C, and the drying time was 1.5 hours;

[0060] 2) Put 1g of Acanthopanax senticosus powder in the extraction tank, add 40mL of deep eutectic solvent, and then carry out constant temperature stirring, the constant temperature is 85°C, the stirring time is 2h, and the stirring speed is 500r / min, to obtain Eleutheroside B of the e...

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Abstract

The invention discloses a synthetic method of a deep eutectic solvent and a method for extracting and purifying eleutheroside B by using the deep eutectic solvent. The synthetic method comprises the following steps: mixing choline chloride, L-proline and water; stirring the mixed components at a constant temperature to synthesize a deep-eutectic solvent, drying the acanthopanax, grinding the acanthopanax into powder, and sieving the powder with a 60-80-mesh sieve; and putting acanthopanax powder into an extraction tank, adding a deep-eutectic solvent, and then stirring at constant temperature to obtain an extracting solution of eleutheroside B; The method for extracting the eleutheroside B in the acanthopanax by using the eutectic solvent and adopting a constant-temperature heating and stirring method is efficient, green, safe and low in cost, and compared with a 70% ethanol solvent, the method has relatively high extraction efficiency and relatively simple and convenient purification process on the eleutheroside B in the acanthopanax.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to a method for synthesizing a deep eutectic solvent and a method for extracting and purifying eleutheroside B using it. Background technique [0002] Acanthopanax senticosus (Rupr.rtMaxim.) Harms] is the dry root and rhizome of Acanthopanax, mainly distributed in Northeast China, and also found in Russia, South Korea, North Korea, Japan and other countries. "Chinese Materia Medica" records that Acanthopanax has the effects of nourishing the liver and kidney; strengthening bones and muscles; diuresis and nourishing the kidney. Modern pharmacological studies have shown that eleutheroside B has excitatory effects on the central nervous system, and its effect is different from that of amphetamine compounds. Eleutheroside B is not only low in toxicity, but also does not disturb normal sleep. Eleutheroside has anti-fatigue effects , stronger than ginseng extract and gin...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634
Inventor 孙长海石鑫磊孙适远
Owner JIAMUSI UNIVERSITY
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