Preparation method and application of targeted mercuric sulfide nanoparticle medicine
A nanoparticle, mercury sulfide technology, applied in the directions of pharmaceutical formulations, drug combinations, drug delivery, etc., to achieve the effects of simple process, good stability, and easy control of process conditions
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Embodiment 1
[0044] Embodiment 1 A kind of preparation method of targeted mercury sulfide nanoparticle medicine
[0045] This embodiment is a preparation method of targeted mercury sulfide nanoparticle medicine, which is prepared by a one-pot method, and is carried out in sequence according to the following steps:
[0046] (11) At 25°C, add 32.5 mg of mercury nitrate into the reduced glutathione solution under vigorous stirring (dissolve the reduced glutathione in purified water in advance, stir vigorously until dissolved), and the stirring speed is 1500rad / s, the stirring time is 20min, the mass ratio of reduced glutathione to mercuric nitrate is 2:1, and the pH is adjusted to 7 with sodium hydroxide to obtain A;
[0047] (12) At 25°C, pass N into A 2 (N 2 The flow rate is 0.03-0.04m 3 / s), and drip sodium sulfide solution (concentration is 1.5mol / L, the mass ratio of sodium sulfide and mercuric nitrate is 1:1250) with the speed of 0.1mL / min in A, after dropping, stop feeding Nitrogen,...
Embodiment 2-4
[0050] The preparation method of embodiment 2-4 targeting type mercury sulfide nanoparticles medicine
[0051] Examples 2-4 are respectively a preparation method of targeted mercury sulfide nanoparticle medicine, the preparation process is similar to that of Example 1, the only difference is that the corresponding technical parameters in the preparation process are different, see the table below for details.
[0052]
[0053]
Embodiment 5
[0054] Embodiment 5 effect test
[0055] The targeted mercury sulfide nanoparticle drugs prepared in Examples 1-4 can be applied to the treatment of psoriasis. In this example, performance experiments will be conducted on the mercury sulfide nanoparticles prepared in the present invention.
[0056] (1) In vitro active oxygen test
[0057] The present invention has carried out in vitro active oxygen test to the mercuric sulfide nanoparticle prepared in embodiment 1-4, and the process of testing adopts prior art means, through in vitro experiment, adds H to the mercuric sulfide nanoparticle solution 2 o 2 , the test results show that the mercuric sulfide nanoparticles prepared by the present invention will have the characteristics of quantum dot aggregation under the action of active oxygen, figure 1 Figure (b) in the middle is the scanning electron microscope image of the sample prepared in Example 1 of the present invention under the action of active oxygen. It can be seen ...
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