Small lentinus edodes strain and cultivation method thereof
A cultivation method and technology of shiitake mushrooms, which are applied to small shiitake mushroom strains and their cultivation fields, can solve problems such as poor taste, inconvenient cooking, and low output, and achieve the effects of storage resistance, fast color change, and strong stress resistance
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Embodiment 1
[0054] 1. The domestication and breeding method of a kind of wild small shiitake mushroom strain, concrete steps:
[0055] 1) Collection of wild shiitake mushrooms: wild shiitake mushrooms that are still fruiting when the highest daytime temperature reaches 35°C, wild shiitake mushrooms that are still fruiting when the daytime minimum temperature reaches 5°C, and the average daytime temperature is 18-25°C. Wild shiitake mushrooms that are still fruiting at ℃; wild shiitake mushrooms that are healthy, free from diseases and insect pests, and about to open umbrellas from 3 different ecological regions;
[0056] 2) Culture medium preparation: use PDA medium, prepare triangular culture flasks according to the number of wild shiitake mushrooms collected, and make corresponding numbers, then put the prepared PDA culture solution into the triangular flask culture bottles, and then undergo routine sterilization , after cooling for later use;
[0057] 3) Isolation of bacterial strains...
Embodiment 2
[0091] The cultivation method of the small shiitake mushroom strains comprises transferring the preserved strains of the shiitake mushroom strains to a sawdust medium for cultivation and fruiting management. The culture temperature is controlled at 18°C, and the sawdust culture medium includes the following components in weight percent: miscellaneous sawdust 73%, wheat bran 25%, sugar 1%, gypsum powder 1%, and the water content of the sawdust culture medium is 65%. When carrying out bagging and fruiting, first spray lime water with a pH value of 12 with water, and after 12 hours, spray heavy water once, start to stew the mushrooms in the shed, and the relative air humidity is 90%.
Embodiment 3
[0093] The cultivation method of the small shiitake mushroom strains comprises transferring the preserved strains of the shiitake mushroom strains to a sawdust medium for cultivation and fruiting management. The culture temperature is 27° C., and the sawdust medium includes the following components in weight percent: miscellaneous sawdust 70%, wheat bran 30%, sugar 0.5%, gypsum powder 1.2%, and the water content of the sawdust medium is 58%. When carrying out bagging and fruiting, first spray lime water with a pH value of 12 with water, and after 12 hours, spray heavy water once, start to stew the mushrooms in the shed, and the relative air humidity is 90%.
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