Small lentinus edodes strain and cultivation method thereof

A cultivation method and technology of shiitake mushrooms, which are applied to small shiitake mushroom strains and their cultivation fields, can solve problems such as poor taste, inconvenient cooking, and low output, and achieve the effects of storage resistance, fast color change, and strong stress resistance

Active Publication Date: 2021-08-31
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are about 6 categories and more than 30 strains of shiitake mushrooms artificially cultivated at home and abroad, but they are all large individual varieties. With the continuous improvement of the quality requirements of modern dietary life, large individual shiitake mushrooms are difficult to make porridge due to high tissue fibrosis. Or porridge, not only poor taste but also inconvenient to cook, so the small individual shiitake mushrooms selected from large individuals have been in short supply, and the price is 3-5 times that of traditional common shiitake mushrooms
However, the small shiitake mushrooms all come from wild or conventional cultivars at present, and the output is very small.

Method used

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  • Small lentinus edodes strain and cultivation method thereof
  • Small lentinus edodes strain and cultivation method thereof
  • Small lentinus edodes strain and cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. The domestication and breeding method of a kind of wild small shiitake mushroom strain, concrete steps:

[0055] 1) Collection of wild shiitake mushrooms: wild shiitake mushrooms that are still fruiting when the highest daytime temperature reaches 35°C, wild shiitake mushrooms that are still fruiting when the daytime minimum temperature reaches 5°C, and the average daytime temperature is 18-25°C. Wild shiitake mushrooms that are still fruiting at ℃; wild shiitake mushrooms that are healthy, free from diseases and insect pests, and about to open umbrellas from 3 different ecological regions;

[0056] 2) Culture medium preparation: use PDA medium, prepare triangular culture flasks according to the number of wild shiitake mushrooms collected, and make corresponding numbers, then put the prepared PDA culture solution into the triangular flask culture bottles, and then undergo routine sterilization , after cooling for later use;

[0057] 3) Isolation of bacterial strains...

Embodiment 2

[0091] The cultivation method of the small shiitake mushroom strains comprises transferring the preserved strains of the shiitake mushroom strains to a sawdust medium for cultivation and fruiting management. The culture temperature is controlled at 18°C, and the sawdust culture medium includes the following components in weight percent: miscellaneous sawdust 73%, wheat bran 25%, sugar 1%, gypsum powder 1%, and the water content of the sawdust culture medium is 65%. When carrying out bagging and fruiting, first spray lime water with a pH value of 12 with water, and after 12 hours, spray heavy water once, start to stew the mushrooms in the shed, and the relative air humidity is 90%.

Embodiment 3

[0093] The cultivation method of the small shiitake mushroom strains comprises transferring the preserved strains of the shiitake mushroom strains to a sawdust medium for cultivation and fruiting management. The culture temperature is 27° C., and the sawdust medium includes the following components in weight percent: miscellaneous sawdust 70%, wheat bran 30%, sugar 0.5%, gypsum powder 1.2%, and the water content of the sawdust medium is 58%. When carrying out bagging and fruiting, first spray lime water with a pH value of 12 with water, and after 12 hours, spray heavy water once, start to stew the mushrooms in the shed, and the relative air humidity is 90%.

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Abstract

The invention discloses a small lentinus edodes strain and a cultivation method thereof, and belongs to the technical field of macro fungus breeding. The small lentinus edodes strain is Lentinuslaedodes F1, and the preservation number of the strain is CCTCC No: M2021365. According to the cultivation method of the small lentinus edodes strain, the preserved strain of the lentinus edodes strain is transferred to a sawdust culture medium for cultivation and fruiting management. The sawdust culture medium comprises the following components in percentage by weight: 70%-80% of weed tree sawdust, 20%-30% of wheat bran, 0.2%-2% of sugar and 0.5%-1.5% of gypsum powder, and the water content of the sawdust culture medium is 55%-75%. The strain is a wide-temperature type small short-cell-age lentinus edodes strain, can realize fruiting all day long annually, is high in yield and good in quality, and has the biotransformation rate of 90% or above; and the blank that wide-temperature varieties and the small lentinus edodes strains are lacked in artificial lentinus edodes cultivation at present is filled up.

Description

technical field [0001] The invention belongs to the technical field of large fungus breeding, and in particular relates to a small mushroom strain and a cultivation method thereof. Background technique [0002] 0002 Shiitake mushrooms (Lentinula edodes), also known as mushrooms, Xiangxin, and winter mushrooms, belong to the Basidiomycetes, Agaricales, Tricholomaceae, and Lentinula genus. The provenance of shiitake mushrooms is mainly distributed in China, North Korea, the Philippines, New Zealand, Nepal and other countries. Mushroom products are the second largest edible mushroom consumption species in the world. Shiitake mushroom cultivation has a long history in our country. According to textual research, it has a history of nearly a thousand years. At present, my country has become the main production and consumption country of shiitake mushrooms in the world, with production accounting for 90% of the world. Shiitake mushroom cultivation has become an important way for f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20C12N1/14C12R1/645
CPCA01G18/40A01G18/20C12N1/14
Inventor 黄伟于富强赵琪陈涵知陈国兰龙元丽
Owner SOUTHWEST FORESTRY UNIVERSITY
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