Insect cpcfc family epidermal protein, coding nucleotide sequence and application thereof

A nucleotide sequence and epidermal protein technology, applied in the field of bioengineering, can solve problems such as unreported research
CN113321718BActive Publication Date: 2022-04-15AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2022-04-15

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Abstract

The invention provides an insect CPCFC family epidermal protein, a coding nucleotide sequence and application thereof, and relates to the technical field of bioengineering. The present invention takes the coleopteran insect, the two-pronged rhinoceros beetle as the research object, from which two CPCFC family protein genes were discovered, named TdCPCFC1 and TdCPCFC2, and the recombinant proteins of the two genes were successfully obtained, which is helpful for understanding the properties of CPCFC-like proteins. As well as understanding the role of CPCFC family epidermal proteins in the assembly of insect epidermis, it provides a new gene resource for the study of CPCFC family epidermal proteins. The invention provides recombinantly expressed insect CPCFC family epidermal protein TdCPCFC1, which can bind metal ions, has reducing properties, can combine with chitin, and can self-assemble to form aggregates, gels, etc., and the formed aggregates and gels have adhesive properties. And other properties, can be used to develop the corresponding biological products.
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Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to insect CPCFC family epidermal proteins, encoding nucleotide sequences and applications thereof. Background technique

[0002] Arthropods are the largest and most abundant group in the animal kingdom, and insects are one of the most important components of arthropods. Insects have excellent adaptability in the survival, reproduction and development of aquatic and terrestrial environments. The structure of their cuticle plays an important role in their successful environmental adaptation. Insect epidermis is an extracellular matrix secreted and organized by epithelial cells, which can protect insects from water loss in the body; at the same time, it serves as a barrier for biological toxins, pathogens and predators; it can also serve as an insect exoskeleton to help insect movement. Insect skin is mainly composed of chitin and protein, which can provide many research dire...

Claims

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