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Preparation method of selenium-rich black wheat malt oligosaccharide peptide and product of selenium-rich black wheat malt oligosaccharide peptide

A wheat malt and oligosaccharide technology is applied in the field of preparation of selenium-enriched triticale malt oligosaccharide peptides, and can solve the problems of being unsuitable for cooking and baking products, limiting the application of germinated wheat, and high amylase activity

Inactive Publication Date: 2021-08-31
HEBEI ZIFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high amylase activity of germinated wheat itself, it is not suitable for cooking and baking products, which limits the application of germinated wheat

Method used

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  • Preparation method of selenium-rich black wheat malt oligosaccharide peptide and product of selenium-rich black wheat malt oligosaccharide peptide
  • Preparation method of selenium-rich black wheat malt oligosaccharide peptide and product of selenium-rich black wheat malt oligosaccharide peptide
  • Preparation method of selenium-rich black wheat malt oligosaccharide peptide and product of selenium-rich black wheat malt oligosaccharide peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment includes the following steps:

[0029] a. Germinate, dry and pulverize selenium-enriched triticale to prepare selenium-enriched triticale malt powder.

[0030] b Mix the selenium-enriched black wheat malt powder and water in step a according to 1:10 (percentage by weight) and then add it to the enzymolysis tank, turn on the agitator, set the speed at 60 rpm, heat to 90° C. with water steam, and Cook for 30 minutes to obtain selenium-enriched triticale mash.

[0031] c The selenium-enriched triticale mash in step b is cooled to 60°C for the first time, the pH meter is opened, and sodium hydroxide is added to the enzymolysis tank with a peristaltic pump to adjust the pH value to 7.0, and then 1wt% amylase ( Accounting for 1% of the weight of selenium-enriched triticale malt powder), saccharification and hydrolysis for 2 hours to obtain saccharification and hydrolysis of selenium-enriched triticale mash.

[0032] d The temperature of the saccharified and ...

Embodiment 2

[0036] This embodiment includes the following steps:

[0037] a. Germinate, dry and pulverize selenium-enriched triticale to prepare selenium-enriched triticale malt powder.

[0038] b Mix the selenium-enriched black wheat malt powder and water in step a according to 1:10 (percentage by weight) and add it to the enzymolysis tank, turn on the agitator, set the speed at 60 rpm, heat to 90° C. with water steam, and Cook for 30 minutes to obtain selenium-enriched triticale mash.

[0039] c The selenium-enriched triticale mash in step b is cooled to 70°C for the first time, the pH meter is opened, and sodium hydroxide is added to the enzymolysis tank with a peristaltic pump to adjust the pH value to 8.0, and then 3wt% amylase ( Accounting for 3% of the weight of selenium-enriched triticale malt powder), saccharification and hydrolysis for 3 hours to obtain saccharification and hydrolysis selenium-enrichment triticale mash.

[0040] d The temperature of the saccharified and hydrolyz...

Embodiment 3

[0044] This embodiment includes the following steps:

[0045] a. Germinate, dry and pulverize selenium-enriched triticale to prepare selenium-enriched triticale malt powder.

[0046] b Mix the selenium-enriched black wheat malt powder and water in step a according to 1:10 (percentage by weight) and then add it to the enzymolysis tank, turn on the agitator, set the speed at 60 rpm, heat to 90° C. with water steam, and Cook for 30 minutes to obtain selenium-enriched triticale mash.

[0047] c The selenium-enriched triticale mash in step b is cooled to 70°C for the first time, the pH meter is opened, and sodium hydroxide is added to the enzymolysis tank with a peristaltic pump to adjust the pH value to 8.0, and then 2wt% amylase ( Calculated according to the weight of selenium-enriched triticale malt powder), saccharified and hydrolyzed for 3 hours to obtain saccharified and hydrolyzed selenium-enriched triticale mash.

[0048] d The temperature of the saccharified and hydrolyz...

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Abstract

The invention provides a preparation method of selenium-rich black wheat malt oligosaccharide peptide. The preparation method comprises the following steps that a, selenium-rich black wheat is germinated, dried, and crushed to obtain selenium-rich black wheat malt powder; b, the selenium-rich black wheat malt powder in the step a is mixed with water, and the mixture is added into an enzymolysis tank to be stirred and heated to obtain selenium-rich black wheat malt pulp; c, the selenium-rich black wheat malt pulp in the step b is cooled for the first time, an alkaline regulator and amylase are added into the enzymolysis tank, and saccharification hydrolysis is carried out to obtain saccharified and hydrolyzed selenium-rich black wheat malt pulp; d, the saccharified and hydrolyzed selenium-rich black wheat malt pulp in the step c is cooled for the second time, and then enzyme is added for enzymolysis to obtain enzymatic hydrolysate; e, heating, enzyme deactivation and centrifugal separation are carried out on the enzymatic hydrolysate in the step d to obtain supernate, and then decoloration, adsorption treatment and nanofiltration are carried out on the supernate to obtain nanofiltration trapped fluid; and f, the nanofiltration trapped fluid in the step e is subjected to vacuum concentration and dried to obtain the selenium-rich black wheat malt oligosaccharide peptide.

Description

technical field [0001] The present application relates to the technical field of food processing, in particular to a method for preparing selenium-enriched triticale maltooligosaccharide peptides and products thereof. Background technique [0002] Selenium is an essential trace element for the human body, and is listed as one of the 15 essential trace elements by the Chinese Nutrition Society. Selenium plays an important role in the physiological and life activities of the human body. A large number of scientific results show that the lack of selenium may lead to disorders of human organs, decreased immunity, and more than 40 kinds of diseases that threaten human health and life are related to human selenium deficiency. Such as cancer, cardiovascular disease, liver disease, cataract, pancreatic disease, diabetes, reproductive system disease, etc. The population of most provinces in China is in selenium-deficient or low-selenium areas, and the incidence rates of tumors, live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/36C07K1/34C07K1/18A23L2/39A23L2/52A23L33/18A23L33/175A23L33/125A23L19/00
CPCC12P21/06C07K1/145C07K1/36C07K1/34C07K1/18A23L2/39A23L2/52A23L33/18A23L33/175A23L33/125A23L19/09A23L19/01A23V2002/00A23V2250/55A23V2250/28A23V2250/038
Inventor 杨广刘奎秀时保兴刘壮
Owner HEBEI ZIFENG BIOTECH
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