Method for detecting ascorbic acid based on gold cluster and TMB colorimetric fluorescence double signals

A technology of ascorbic acid and gold clusters, applied in the field of fluorescence analysis and detection, can solve the problems of large background signal interference and large environmental fluctuation, and achieve the effects of low detection limit, good accuracy and mature synthesis technology.

Inactive Publication Date: 2021-08-31
四川中科微纳科技有限公司 +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem solved by the present invention is that the existing single fluorescence or colorimetric detection of ascorbic acid has large background signal interference and large environmental fluctuations

Method used

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  • Method for detecting ascorbic acid based on gold cluster and TMB colorimetric fluorescence double signals
  • Method for detecting ascorbic acid based on gold cluster and TMB colorimetric fluorescence double signals
  • Method for detecting ascorbic acid based on gold cluster and TMB colorimetric fluorescence double signals

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Effect test

Embodiment 1

[0040] Preparation of AuNCs of the present invention: Add 5ml HAuCl to a clean round bottom flask 4 4H 2 O (20mM), 1.5mL of freshly prepared glutathione solution (100mM) and 43.5mL of ultrapure water, these solutions were stirred at room temperature for 30 minutes to mix thoroughly, and then the mixed solution was heated to 70°C, and After being kept at high temperature for 48 hours, the obtained AuNCs solution was light yellow under visible light, and emitted red fluorescence under 365nm ultraviolet light. It was stored at 4°C and used directly for subsequent experiments.

Embodiment 2

[0042] MnO of the present invention 2 Preparation of nanosheets: 1.0g KMnO4 and 0.15g MnSO 4 ·H 2O was dissolved in 70 mL of deionized water, stirred for 15 minutes to dissolve the mixture completely, then the mixture was placed in a polytetrafluoroethylene (PTFE)-lined hydrothermal reactor, and reacted at 200 °C for 12 hours, and the final solid product was After centrifugation and washing three times to remove possible impurities, the washed solids were finally placed in an oven at 100°C for 12 hours, and the obtained material was preserved and stored for later use.

Embodiment 3

[0044] Establish standard curves for fluorescence detection of ascorbic acid and colorimetric detection of ascorbic acid: first, prepare standard solutions of ascorbic acid with a concentration gradient of 0-200 μM, then mix 3ml TMB solution (2mM) and 600μL MnO 2 The suspension of nanosheets (4 mg / mL) was sequentially added to 80 mL of HAc-NaAc (0.2 mM) buffer solution at pH 4, stirred and incubated at room temperature for 1 hour, and the mixture was filtered to remove MnO 2 Nanosheets, save the obtained blue oxTMB solution for later use, mix 200μLAuNCs (200μM), 1mL oxTMB (72μM, converted from TMB) and AA standard solutions of different concentrations, and the mixed solution is incubated at room temperature for 10 minutes before fluorescence value and colorimetric value tend to be stable, measure the relative fluorescence intensity and ultraviolet absorption intensity, respectively record the fluorescence emission peak and ultraviolet absorption peak of the solution at 572nm an...

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Abstract

The invention discloses a method for detecting ascorbic acid based on fluorescence and colorimetric double signals, and belongs to the technical field of fluorescence analysis and detection. In the prior art, single fluorescence or colorimetric detection of ascorbic acid is adopted, which has the problems of large background signal interference and large environmental fluctuation. The detection method comprises the following steps: mixing a TMB solution and nano MnO2, carrying out oxidation incubation, filtering to remove the nano MnO2 after incubation to obtain a blue oxTMB solution, mixing the oxTMB solution, an AuNCs solution and an AA-containing to-be-detected solution for reaction, and detecting AA by measuring relative fluorescence intensity and ultraviolet absorption intensity based on a fluorescence inner filtration effect. According to the invention, detection can be carried out without operation of expensive instruments and professional training personnel, and the method has the advantages of time and labor saving, low detection limit, good accuracy and simple and rapid detection.

Description

technical field [0001] The invention belongs to the technical field of fluorescence analysis and detection, and specifically relates to a method for detecting ascorbic acid based on gold clusters and TMB colorimetric fluorescence dual-signal detection Background technique [0002] Ascorbic acid, also known as vitamin C, plays a very important role in maintaining the normal metabolism of the human body. It has a strong reducing ability and is an important and efficient antioxidant in the human body. In addition, ascorbic acid plays an irreplaceable role in promoting the synthesis of human collagen and neurotransmitters, promoting the formation of antibodies and the absorption of iron ions. Ascorbic acid also has the ability to scavenge free radicals and prevent various diseases. Scurvy is caused when the human body is severely deficient in ascorbic acid. Since humans cannot synthesize ascorbic acid independently, ascorbic acid is mainly obtained from food or health care med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/33G01N21/78G01N15/14G01N15/00
CPCG01N15/00G01N15/14G01N15/1434G01N21/33G01N21/64G01N21/6428G01N21/643G01N21/6458G01N21/78G01N2015/0038G01N2015/0065G01N2015/149G01N2021/6417G01N2021/6495G01N2021/6497
Inventor 霍峰蒋志丁豪王显祥
Owner 四川中科微纳科技有限公司
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